IDH3A Antibody

Datasheet

Code	CSB-PA010991LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: HepG2 whole cell lysat, Jurkat whole cell lysate, Rat brain tissue, Mouse brain tissue, Mouse liver tissue All lanes: IDH3A antibody at 3.4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 40, 32 kDa Observed band size: 40 kDa Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA010991LA01HU at dilution of 1:100 Immunofluorescent analysis of A549 cells using CSB-PA010991LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) Immunoprecipitating IDH3A in HepG2 whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA010991LA01HU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA010991LA01HU (6µg) + HepG2 whole cell lysate (500µg) Lane 3: HepG2 whole cell lysate (20µg)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) IDH3A Polyclonal antibody

Uniprot No.

P50213

Target Names

IDH3A

Alternative Names

H IDH alpha antibody; HIDH alpha antibody; IDH3A antibody; IDH3A_HUMAN antibody; Isocitrate dehydrogenase (NAD+) alpha chain precursor antibody; Isocitrate dehydrogenase [NAD] subunit alpha antibody; Isocitrate dehydrogenase [NAD] subunit alpha mitochondrial antibody; Isocitrate dehydrogenase 3 (NAD+) alpha antibody; Isocitrate dehydrogenase antibody; Isocitric dehydrogenase antibody; Isocitric dehydrogenase subunit alpha antibody; mitochondrial antibody; NAD antibody; NAD(+)-specific ICDH subunit alpha antibody; NAD(H) specific isocitrate dehydrogenase alpha subunit precursor antibody; NAD+ specific ICDH antibody; NADsubunit alpha mitochondrial precursor antibody; OTTHUMP00000184906 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Human Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial protein (28-108AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The IDH3A Antibody (Product code: CSB-PA010991LA01HU) is Non-conjugated. For IDH3A Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA010991LB01HU	IDH3A Antibody, HRP conjugated	ELISA
FITC	CSB-PA010991LC01HU	IDH3A Antibody, FITC conjugated
Biotin	CSB-PA010991LD01HU	IDH3A Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200
IP	1:200-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Catalytic subunit of the enzyme which catalyzes the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers.

Gene References into Functions

The silencing of IDH3a significantly delayed tumor growth by suppressing the HIF-1-mediated Warburg effect and angiogenesis. PMID: 25531325
for IDH, a normal alpha subunit is required for catalytic activity and alpha-Arg88 likely participates in the isocitrate site, whereas the beta and gamma subunits have roles in the nucleotide functions of this allosteric enzyme PMID: 14555658
active sites of the human NAD-IDH are shared between alpha and gamma subunits and between alpha and beta subunits PMID: 16737955
Asp181 is essential for nicotinamide-adenine dinucleotide (NAD)-specific IDH alpha subunit catalysis and may also facilitate the binding of substrates. PMID: 17432878

Subcellular Location

Mitochondrion.

Protein Families

Isocitrate and isopropylmalate dehydrogenases family

Database Links

HGNC: 5384

OMIM: 601149

KEGG: hsa:3419

STRING: 9606.ENSP00000299518

UniGene: Hs.591110

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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