ELP1 Antibody

Datasheet

Citations (1)

Code	CSB-PA011571LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate All lanes: IKBKAP antibody at 5.3μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 151 kDa Observed band size: 151 kDa IHC image of CSB-PA011571LA01HU diluted at 1:600 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA011571LA01HU diluted at 1:600 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA011571LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunoprecipitating IKBKAP in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA011571LA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA011571LA01HU (6μg) + Hela whole cell lysate (1mg) Lane 3: Hela whole cell lysate (20μg)
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Product Details
Citations
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ELP1 Polyclonal antibody

Uniprot No.

O95163

Target Names

ELP1

Alternative Names

DKFZp781H1425 antibody; DYS antibody; Dysautonomia (Riley Day syndrome hereditary sensory autonomic neuropathy type III) antibody; Elongator complex protein 1 antibody; ELP 1 antibody; ELP1 antibody; ELP1_HUMAN antibody; FD antibody; FLJ12497 antibody; IKAP antibody; IkappaB kinase complex associated protein antibody; IkappaB kinase complex-associated protein antibody; ikbkap antibody; IKI 3 antibody; IKI3 antibody; IKK complex associated protein antibody; IKK complex-associated protein antibody; Inhibitor of kappa light polypeptide gene enhancer in B cells kinase complex associated protein antibody; OTTHUMP00000063889 antibody; p150 antibody; TOT 1 antibody; TOT1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Elongator complex protein 1 protein (1107-1258AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ELP1 Antibody (Product code: CSB-PA011571LA01HU) is Non-conjugated. For ELP1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA011571LB01HU	ELP1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA011571LC01HU	ELP1 Antibody, FITC conjugated
Biotin	CSB-PA011571LD01HU	ELP1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000
IF	1:50-1:200
IP	1:200-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Citations

Genetic modeling of ELP1-associated Sonic hedgehog medulloblastoma identifies MDM2 as a selective therapeutic target ST Ahmad, Y Li, J Garcia-Lopez, BL Gudenas,Cancer Cell,2025

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Target Background

Function

Component of the RNA polymerase II elongator complex, a multiprotein complex associated with the RNA polymerase II (Pol II) holoenzyme, and which is involved in transcriptional elongation. The elongator complex catalyzes formation of carboxymethyluridine in the wobble base at position 34 in tRNAs. Involved in neurogenesis. Regulates the migration and branching of projection neurons in the developing cerebral cortex, through a process depending on alpha-tubulin acetylation. May act as a scaffold protein that may assemble active IKK-MAP3K14 complexes (IKKA, IKKB and MAP3K14/NIK).

Gene References into Functions

overexpression of miR-203a-3p leads to a decrease of NOVA1, counter-balanced by an increase of IKAP, supporting a potential interaction between NOVA1 and IKAP. PMID: 27483351
IKAP might be a vesicular like protein that might be involved in neuronal transport in hESC derived PNS neurons PMID: 26437462
The formation of the Elp1 dimer contributes to its stability in vitro and in vivo and is required for the assembly of human Elongator complexes. PMID: 26261306
IKBKAP mRNA levels decreased during a familial dysautonomia crisis and returned to baseline after recovery. The cause-and-effect relationship is unclear. PMID: 24268683
Phosphatidylserine increases IKBKAP levels in a humanized knock-in IKBKAP mouse model for Familial dysautonomia. PMID: 23515154
Digoxin-mediated repression of SRSF3 expression plays a role in the digoxin-mediated inclusion of exon 20 in the IKBKAP transcript generated from the familial dysautonomia mutant allele. PMID: 23711097
Combined treatment with epigallocatechin gallate and genistein synergistically upregulates wild-type IKBKAP-encoded RNA and protein levels in familial dysautonomia-derived cells. PMID: 22495984
IKAP plays pleiotropic roles in both the peripheral and central nervous systems PMID: 22384137
IKAP/hELP1 deficiency has an effect on gene expression in differentiating neuroblastoma cells, and possibly on familial dysautonomia PMID: 21559466
IKK complex-associated protein deficiency upregulates the microtubule destabilizing protein SCG10 and, in parallel, disorganizes the cytoskeleton PMID: 21273291
Phosphatidylserine increases IKBKAP levels in familial dysautonomia cells PMID: 21209961
IKAP regulates contactin levels for appropriate cell-cell adhesion that could modulate neuronal growth of neurons during development PMID: 20671422
IKAP is critical for the development of afferent baroreflex pathways and has therapeutic implications in the management of these patients. PMID: 21098405
IKBKAP is a candidate gene for Hirschsprung's disease and was mapped to chromosome 9q31 locus. PMID: 20361209
novel role for the I kappa B kinase complex-associated protein (IKAP) in the regulation of activation of the mammalian stress response via the c-Jun N-terminal kinase (JNK)-signaling pathway PMID: 12058026
Genetics of familial dysautonomia; tissue-specific expression of a splicing mutation (REVIEW) PMID: 12102458
Tissue-specific reduction in splicing efficiency of this protein is due to the major mutation associated with familial dysautonomia. PMID: 12577200
The study results suggest that the polymorphisms in the coding region of the IKAP gene are unlikely to contribute to atopic disease risk in the Czech population. PMID: 12774215
whereas IKBKAP (Elongator) is recruited to both target and nontarget genes, only target genes display histone H3 hypoacetylation and progressively lower RNAPII density through the coding region in familial dysautonomia cells PMID: 16713582
Neurodevelopmental disease familial dysautonomia (FD)caused by a single-base change in the 5' splice site (5'ss) of intron 20 in the IKBKAP gene (c.2204+6T>C). PMID: 16964593
investigated the nature of the FD splicing defect and the mechanism by which kinetin improves exon inclusion PMID: 17206408
IKAP/hELP1 may play a role in oligodendrocyte differentiation and/or myelin formation. PMID: 17591626
description of a humanized IKBKAP transgenic mouse that models a tissue-specific human splicing defect PMID: 17644305
IKBKAP may have a role in familial dysautonomia PMID: 18091349
Evidence for the role of the cytosolic interactions of IKAP in cell adhesion and migration, and support the notion that cell-motility deficiencies could contribute to familial dysautonomia. PMID: 18303054
IKAP is crucial for both vascular and neural development during embryogenesis and that protein function is conserved between mouse and human. PMID: 19015235

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Involvement in disease

Neuropathy, hereditary sensory and autonomic, 3 (HSAN3)

Subcellular Location

Cytoplasm. Nucleus.

Protein Families

ELP1/IKA1 family

Database Links

HGNC: 5959

OMIM: 223900

KEGG: hsa:8518

STRING: 9606.ENSP00000363779

UniGene: Hs.494738

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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