INPP5B Antibody

Datasheet

Code	CSB-PA011734LA01HU
Size	US$166
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Image	IHC image of CSB-PA011734LA01HU diluted at 1:400 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA011734LA01HU diluted at 1:400 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA011734LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) INPP5B Polyclonal antibody

Uniprot No.

P32019

Target Names

INPP5B

Alternative Names

4 antibody; 5-trisphosphate 5-phosphatase antibody; 5PTase antibody; 75 kDa inositol polyphosphate 5 phosphatase antibody; 75 kDa inositol polyphosphate-5-phosphatase antibody; 75kDa antibody; EC 3.1.3.36 antibody; I5P2_HUMAN antibody; Inositol polyphosphate 5-phosphatase II antibody; Inositol polyphosphate-5-phosphatase B antibody; Inositol polyphosphate-5-phosphatase; 75kDa antibody; INPP5B antibody; Inpp5b protein antibody; INPP5P antibody; MGC65156 antibody; MGC71303 antibody; OTTHUMP00000004726 antibody; OTTHUMP00000004727 antibody; OTTHUMP00000004728 antibody; Phosphoinositide 5 phosphatase antibody; Phosphoinositide 5-phosphatase antibody; Type II inositol 1,4,5 trisphosphate 5 phosphatase antibody; Type II inositol-1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Type II inositol 1,4,5-trisphosphate 5-phosphatase protein (78-178AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The INPP5B Antibody (Product code: CSB-PA011734LA01HU) is Non-conjugated. For INPP5B Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA011734LB01HU	INPP5B Antibody, HRP conjugated	ELISA
FITC	CSB-PA011734LC01HU	INPP5B Antibody, FITC conjugated
Biotin	CSB-PA011734LD01HU	INPP5B Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Hydrolyzes phosphatidylinositol 4,5-bisphosphate (PtIns(4,5)P2) and the signaling molecule phosphatidylinositol 1,4,5-trisphosphate (PtIns(1,4,5)P3), and thereby modulates cellular signaling events.

Gene References into Functions

OCRL-mutated fibroblasts from patients with Dent-2 disease exhibit INPP5B-independent phenotypic variability relatively to Lowe syndrome cells PMID: 25305077
The crystal structures of human INPP5B in complex with phosphoinositide substrate analogs revealed a membrane interaction patch likely to assist in sequestering substrates from the lipid bilayer. PMID: 24704254
study found mouse Inpp5b and human INPP5B differ in their transcription, splicing and amino acid sequence; observations form foundation for analyzing the functional basis for the difference in how Inpp5b and INPP5B compensate for loss of Ocrl function PMID: 20872266
The homologous phosphatase Inpp5b was unable to complement the Ocrl1-dependent cell migration defect. PMID: 19700499
INPP5B is also localised to the early secretory pathway including the Golgi apparatus and ER-to-Golgi intermediate compartment (ERGIC). PMID: 17956944
The NH2-terminal region of OCRL, but not of INPP5B, binds clathrin heavy chain. PMID: 19536138

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Subcellular Location

Cytoplasm, cytosol. Endoplasmic reticulum-Golgi intermediate compartment. Early endosome membrane. Membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle, phagosome membrane. Golgi apparatus.

Protein Families

Inositol 1,4,5-trisphosphate 5-phosphatase type II family

Tissue Specificity

Platelets.

Database Links

HGNC: 6077

OMIM: 147264

KEGG: hsa:3633

STRING: 9606.ENSP00000362115

UniGene: Hs.449942

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Phosphatidylinositol signaling system

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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