Recombinant Human Type II inositol 1,4,5-trisphosphate 5-phosphatase (INPP5B), partial

Code CSB-YP011734HU
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Source Yeast
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Code CSB-EP011734HU
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Source E.coli
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Code CSB-EP011734HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP011734HU
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Source Baculovirus
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Code CSB-MP011734HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
INPP5B
Uniprot No.
Alternative Names
4; 5-trisphosphate 5-phosphatase; 5PTase; 75 kDa inositol polyphosphate 5 phosphatase ; 75 kDa inositol polyphosphate-5-phosphatase; 75kDa; EC 3.1.3.36; I5P2_HUMAN; Inositol polyphosphate 5-phosphatase II ; Inositol polyphosphate-5-phosphatase B; Inositol polyphosphate-5-phosphatase; 75kDa; INPP5B; Inpp5b protein ; INPP5P; MGC65156; MGC71303; OTTHUMP00000004726; OTTHUMP00000004727; OTTHUMP00000004728; Phosphoinositide 5 phosphatase; Phosphoinositide 5-phosphatase; Type II inositol 1,4,5 trisphosphate 5 phosphatase; Type II inositol-1
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Hydrolyzes phosphatidylinositol 4,5-bisphosphate (PtIns(4,5)P2) and the signaling molecule phosphatidylinositol 1,4,5-trisphosphate (PtIns(1,4,5)P3), and thereby modulates cellular signaling events.
Gene References into Functions
  1. OCRL-mutated fibroblasts from patients with Dent-2 disease exhibit INPP5B-independent phenotypic variability relatively to Lowe syndrome cells PMID: 25305077
  2. The crystal structures of human INPP5B in complex with phosphoinositide substrate analogs revealed a membrane interaction patch likely to assist in sequestering substrates from the lipid bilayer. PMID: 24704254
  3. study found mouse Inpp5b and human INPP5B differ in their transcription, splicing and amino acid sequence; observations form foundation for analyzing the functional basis for the difference in how Inpp5b and INPP5B compensate for loss of Ocrl function PMID: 20872266
  4. The homologous phosphatase Inpp5b was unable to complement the Ocrl1-dependent cell migration defect. PMID: 19700499
  5. INPP5B is also localised to the early secretory pathway including the Golgi apparatus and ER-to-Golgi intermediate compartment (ERGIC). PMID: 17956944
  6. The NH2-terminal region of OCRL, but not of INPP5B, binds clathrin heavy chain. PMID: 19536138

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Subcellular Location
Cytoplasm, cytosol. Endoplasmic reticulum-Golgi intermediate compartment. Early endosome membrane. Membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle, phagosome membrane. Golgi apparatus.
Protein Families
Inositol 1,4,5-trisphosphate 5-phosphatase type II family
Tissue Specificity
Platelets.
Database Links

HGNC: 6077

OMIM: 147264

KEGG: hsa:3633

STRING: 9606.ENSP00000362115

UniGene: Hs.449942

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