INPP5E Antibody

Datasheet

Code	CSB-PA873641LA01HU
Size	US$166
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Image	IHC image of CSB-PA873641LA01HU diluted at 1:400 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA873641LA01HU diluted at 1:400 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA873641LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) INPP5E Polyclonal antibody

Uniprot No.

Q9NRR6

Target Names

INPP5E

Alternative Names

5-bisphosphate 5-phosphatase antibody; 72 kDa inositol polyphosphate 5-phosphatase antibody; Inositol polyphosphate 5 phosphatase antibody; INP5E_HUMAN antibody; Inpp5e antibody; Phosphatidylinositol (4,5) bisphosphate 5 phosphatase antibody; Phosphatidylinositol 4 antibody; Phosphatidylinositol polyphosphate 5 phosphatase type IV antibody; Phosphatidylinositol polyphosphate 5-phosphatase type IV antibody; PPI5PIV antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human 72 kDa inositol polyphosphate 5-phosphatase protein (1-133AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The INPP5E Antibody (Product code: CSB-PA873641LA01HU) is Non-conjugated. For INPP5E Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA873641LB01HU	INPP5E Antibody, HRP conjugated	ELISA
FITC	CSB-PA873641LC01HU	INPP5E Antibody, FITC conjugated
Biotin	CSB-PA873641LD01HU	INPP5E Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3), phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Specific for lipid substrates, inactive towards water soluble inositol phosphates. Plays an essential role in the primary cilium by controlling ciliary growth and phosphoinositide 3-kinase (PI3K) signaling and stability.

Gene References into Functions

miR598 contributed to cell proliferation and cell cycle progression in colorectal carcinoma by targeting INPP5E. PMID: 29257251
INPP5E associates with the N-terminus of RPGR and trafficking of INPP5E to cilia is dependent upon the ciliary localization of RPGR. PMID: 28172980
we identify Inpp5e as an essential inhibitor of the PI3K/Akt/mTORC1 signaling axis in renal epithelial cells, and demonstrate a critical role for Inpp5e-dependent mTORC1 regulation in Polycystic kidney disease (PKD) suppression PMID: 27056978
ARL13B regulates IFT-A-mediated retrograde protein trafficking within cilia through its interaction with INPP5E. PMID: 27927754
In neuronal cells, INPP5E knockdown strongly inhibited autophagy by impairing the autophagosome-lysosome fusion step. PMID: 27340123
INPP5E localizes to centrosomes, chromosomes, and kinetochores in early mitosis and shuttles to the midzone spindle at mitotic exit. PMID: 28031327
INPP5E is an essential point of convergence between Hedgehog and phosphoinositide signaling at cilia that maintains transition zone function and Hedgehog-dependent embryonic development. PMID: 27998989
PIPKIgamma and INPP5E localize to the centrosome and coordinate the initiation of ciliogenesis. PMID: 26916822
These findings establish the first direct link between AURKA and phosphoinositide signaling and suggest that the function of INPP5E in cilia is at least partly mediated by its interactions with AURKA PMID: 25395580
Proteomic analysis identified INPP5E, whose mutations also lead to Joubert syndrome as novel prenyl-dependent cargo of PDE6D. Mutant PDE6D shows reduced binding to INPP5E, which fails to localize to primary cilia in patient fibroblasts and tissues. PMID: 24166846
Identification of 12 different INPP5E mutations in patients with Joubert syndrome with an overall 2.7% mutation frequency. PMID: 23386033
findings indicate that ARL13B, INPP5E, PDE6D, and CEP164 form a distinct functional network that is involved in JBTS and NPHP but independent of the ones previously defined by NPHP and MKS proteins PMID: 23150559
INPP5E mutations cause primary cilium signaling defects, ciliary instability and ciliopathies in humans. PMID: 19668215
Mutations in INPP5E, encoding inositol polyphosphate-5-phosphatase E, link phosphatidyl inositol signaling to the ciliopathies. PMID: 19668216
Functional analysis of the mouse counterpart. PMID: 10806194

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Involvement in disease

Joubert syndrome 1 (JBTS1); Mental retardation, truncal obesity, retinal dystrophy, and micropenis (MORMS)

Subcellular Location

Cytoplasm, cytoskeleton, cilium axoneme. Golgi apparatus, Golgi stack membrane; Peripheral membrane protein; Cytoplasmic side. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, ruffle. Cytoplasm. Nucleus.

Protein Families

Inositol 1,4,5-trisphosphate 5-phosphatase type IV family

Tissue Specificity

Detected in brain, heart, pancreas, testis and spleen.

Database Links

HGNC: 21474

OMIM: 213300

KEGG: hsa:56623

STRING: 9606.ENSP00000360777

UniGene: Hs.120998

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Phosphatidylinositol signaling system

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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