INSIG1 Antibody

Code CSB-PA011745LA01HU
Size US$166
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  • Western Blot
    Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate
    All lanes: INSIG1 antibody at 2.8µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 30, 18 kDa
    Observed band size: 46 kDa

  • Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA011745LA01HU at dilution of 1:100

  • Immunofluorescent analysis of MCF-7 cells using CSB-PA011745LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) INSIG1 Polyclonal antibody
Uniprot No.
Target Names
INSIG1
Alternative Names
INSIG1; Insulin-induced gene 1 protein; INSIG-1
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Insulin-induced gene 1 protein (1-87AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The INSIG1 Antibody (Product code: CSB-PA011745LA01HU) is Non-conjugated. For INSIG1 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA011745LB01HU INSIG1 Antibody, HRP conjugated ELISA
FITC CSB-PA011745LC01HU INSIG1 Antibody, FITC conjugated
Biotin CSB-PA011745LD01HU INSIG1 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Oxysterol-binding protein that mediates feedback control of cholesterol synthesis by controlling both endoplasmic reticulum to Golgi transport of SCAP and degradation of HMGCR. Acts as a negative regulator of cholesterol biosynthesis by mediating the retention of the SCAP-SREBP complex in the endoplasmic reticulum, thereby blocking the processing of sterol regulatory element-binding proteins (SREBPs) SREBF1/SREBP1 and SREBF2/SREBP2. Binds oxysterol, including 25-hydroxycholesterol, regulating interaction with SCAP and retention of the SCAP-SREBP complex in the endoplasmic reticulum. In presence of oxysterol, interacts with SCAP, retaining the SCAP-SREBP complex in the endoplasmic reticulum, thereby preventing SCAP from escorting SREBF1/SREBP1 and SREBF2/SREBP2 to the Golgi. Sterol deprivation or phosphorylation by PCK1 reduce oxysterol-binding, disrupting the interaction between INSIG1 and SCAP, thereby promoting Golgi transport of the SCAP-SREBP complex, followed by processing and nuclear translocation of SREBF1/SREBP1 and SREBF2/SREBP2. Also regulates cholesterol synthesis by regulating degradation of HMGCR: initiates the sterol-mediated ubiquitin-mediated endoplasmic reticulum-associated degradation (ERAD) of HMGCR via recruitment of the reductase to the ubiquitin ligases AMFR/gp78 and/or RNF139. Also regulates degradation of SOAT2/ACAT2 when the lipid levels are low: initiates the ubiquitin-mediated degradation of SOAT2/ACAT2 via recruitment of the ubiquitin ligases AMFR/gp78.
Gene References into Functions
  1. INSIG1 variation may contribute to statin-induced changes in plasma triglycerides in a sex-specific manner. PMID: 26927283
  2. argue that precursor miR-122 molecules modulate polyadenylation site usage in Insig1 mRNAs, resulting in down-regulation of Insig1 protein abundance PMID: 28928276
  3. Our findings suggest that miR-92a may affect cholesterol metabolism by repressing insig1, resulting in raised intracellular cholesterol levels and Golgi volume and hence enhanced protein secretion. PMID: 28146316
  4. identified interaction of three genes in INSIG-SCAP-SREBP pathway on risk of obesity, revealing that these genes affect obesity more likely through a complex interaction pattern than single gene effect. PMID: 25028659
  5. Data show the essential role of PPARgamma and PPARgamma coactivator 1alpha (PGC-1alpha) in up-regulating Insig-1/2 expression, defining a mechanistic pathway triggered by CD36, and leading to cholesterol depletion in hepatocytes. PMID: 24371122
  6. Our results indicated that the dysregulation of INSIG1 and SREBF1 caused by ART were observed not only in the fetus but also in the placenta, primarily in the ICSI group PMID: 24484994
  7. No significant associations were found between polymorphisms of INSIG1 gene and metabolic syndrome; however, INSIG1 and INSIG2 interactions were found in the significant 3-locus and 4-locus gene-gene interaction models. PMID: 20877301
  8. Observational study of gene-disease association, gene-gene interaction, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 20877301
  9. From genetic association studies, SNPs in INSIG1 (including the promoter region) influence hypertriglyceridemia. PMID: 19965593
  10. Common variation in INSIG1 is unlikely to have a major effect on risk of type 2 diabetes risk in white Europeans. PMID: 20444954
  11. These data suggest that p97 recruits proteasomes to polytopic ER proteins, such as Insig-1, even before they are extracted from membranes. PMID: 19815544
  12. INSIG1 and p41 Arp2/3 complex (p41-Arc)reduced expression might be involved in gastric cancer development or progression PMID: 12115587
  13. INSIG-1 plays a role in regulating cholesterol concentration in human cells PMID: 12242342
  14. Data show that Insig-1 appears to play an essential role in the sterol-mediated trafficking of two proteins with sterol-sensing domains, HMG CoA reductase and SCAP. PMID: 12535518
  15. insig-1 expression restricts lipogenesis in mature adipocytes and blocks differentiation in preadipocytes PMID: 12869692
  16. data indicate that short segments at the N and C termini of insulin induced gene 1(Insig-1) face the cytosol with most of the protein buried within the membrane, forming six transmembrane segments PMID: 14660594
  17. nSREBPs are essential for high levels of lipid synthesis in the liver and indicate that Insig's modulate nSREBP levels by binding and retaining SCAP in the ER. PMID: 15085196
  18. Insig-1 has a role in feedback control of lipid synthesis in cultured cells PMID: 15247248
  19. Insig-1 has a role in activation of sterol regulatory element-binding protein induced by cellular stress PMID: 15304479
  20. Insig is required for sterol-mediated inhibition of Scap/SREBP binding to COPII proteins in vitro PMID: 15899885
  21. Results identify gp78 as the E3 that initiates sterol-accelerated degradation of reductase, and Insig-1 as a bridge between gp78/VCP and the reductase substrate. PMID: 16168377
  22. Under conditions of mild steroid depletion, the failure to ubiquinate Insig-1 causes a delay in release of the Scap/sterol regulatory element binding protein-2, but it does not abolish it. PMID: 16399501
  23. degradation of ubiquitinated Insigs is controlled by serine residues flanking the sites of ubiquitination PMID: 16549805
  24. These studies identify a aspartic acid residue that is crucial for the function of Insig1 and Insig2 proteins in regulating cholesterol homeostasis in mammalian cells. PMID: 16606821
  25. Insig-1 sterol-regulated degradation is mediated by the membrane-bound ubiquitin ligase gp78 PMID: 17043353
  26. Population studies demonstrate that INSIG1 plays a role in glucose homeostasis. PMID: 17106696
  27. Activation of CAR and PXR in mouse liver results in activation of Insig-1 along with reduced protein levels of the active form of sterol regulatory element binding protein 1 (Srebp-1). PMID: 18187584
  28. Unsaturated fatty acid-mediated stabilization of Insig-1 enhances the ability of sterols to inhibit proteolytic activation of SREBP-1, which activates transcription of genes involved in fatty acid synthesis PMID: 18835813
  29. The INSIG1 gene, not the INSIG2 gene, associated with coronary heart disease: tagSNPs and haplotype-based association study. PMID: 18989534
  30. Studies define Insig-1 and Insig-2 as the minimal requirement for sterol-accelerated degradation of the membrane domain of reductase. PMID: 19638338
  31. Observational study of gene-disease association and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 18195716

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Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein.
Protein Families
INSIG family
Tissue Specificity
Expressed in all tissues tested with highest expression in the liver.
Database Links

HGNC: 6083

OMIM: 602055

KEGG: hsa:3638

STRING: 9606.ENSP00000344741

UniGene: Hs.520819

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