IQGAP2 Antibody

Datasheet
Code CSB-PA615701LA01HU
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Size US$299
Uniprot No. Q13576
Image
  • IHC image of CSB-PA615701LA01HU diluted at 1:500 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of HepG2 cells with CSB-PA615701LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunogen Recombinant Human Ras GTPase-activating-like protein IQGAP2 protein (1245-1395AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, IHC, IF; Recommended dilution: IHC:1:500-1:1000, IF:1:50-1:200
Relevance Binds to activated CDC42 and RAC1 but does not seem to stimulate their GTPase activity. Associates with calmodulin.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Ras GTPase-activating-like protein IQGAP2, IQGAP2
Immunogen Species Human
Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Research Area Signal Transduction
Target Names IQGAP2
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Binds to activated CDC42 and RAC1 but does not seem to stimulate their GTPase activity. Associates with calmodulin.
Tissue Specificity Isoform 2 expression is enhanced in testis.
Database Links

HGNC: 6111

OMIM: 605401

KEGG: hsa:10788

STRING: 9606.ENSP00000274364

UniGene: Hs.291030

Pathway Regulation of actin cytoskeleton

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