KRT7 Antibody

Datasheet
Code CSB-PA012564LA01HU
See More Details Free Antibody trial simple
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Uniprot No. P08729
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  • Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA012564LA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human cervical cancer using CSB-PA012564LA01HU at dilution of 1:100

  • Immunofluorescent analysis of HepG2 cells using CSB-PA012564LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Western Blot
    Positive WB detected in: Hela whole cell lysate
    All lanes: KRT7 antibody at 3μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 52 kDa
    Observed band size: 52 kDa

  • Immunoprecipitating KRT7 in HepG2 whole cell lysate
    Lane 1: Rabbit control IgG (1μg) instead of CSB-PA012564LA01HU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-PA012564LA01HU (8μg) + HepG2 whole cell lysate (500μg)
    Lane 3: HepG2 whole cell lysate (10μg)

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
Immunogen Recombinant Human Keratin, type II cytoskeletal 7 protein (2-469AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC, IF, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000
Relevance Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Keratin, type II cytoskeletal 7 (Cytokeratin-7) (CK-7) (Keratin-7) (K7) (Sarcolectin) (Type-II keratin Kb7), KRT7, SCL
Immunogen Species Human
Research Area Signal Transduction
Target Names KRT7
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
Subcellular Location Cytoplasm
Protein Families Intermediate filament family
Tissue Specificity Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.
Database Links

HGNC: 6445

OMIM: 148059

KEGG: hsa:3855

STRING: 9606.ENSP00000329243

UniGene: Hs.411501

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