KTI12 Antibody

Datasheet

Code	CSB-PA839314LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, SH-SY5Y whole cell lysate, 293T whole cell lysate, U87 whole cell lysate, PC-3 whole cell lysate, Rat brain tissue All lanes: KTI12 antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 39 kDa Observed band size: 39 kDa IHC image of CSB-PA839314LA01HU diluted at 1:300 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA839314LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) KTI12 Polyclonal antibody

Uniprot No.

Q96EK9

Target Names

KTI12

Alternative Names

KTI12 antibody; SBBI81 antibody; Protein KTI12 homolog antibody

Raised in

Rabbit

Species Reactivity

Human, Rat

Immunogen

Recombinant Human Protein KTI12 homolog protein (110-204AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The KTI12 Antibody (Product code: CSB-PA839314LA01HU) is Non-conjugated. For KTI12 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA839314LB01HU	KTI12 Antibody, HRP conjugated	ELISA
FITC	CSB-PA839314LC01HU	KTI12 Antibody, FITC conjugated
Biotin	CSB-PA839314LD01HU	KTI12 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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