LARS Antibody

Datasheet

Code	CSB-PA873736LA01HU
Size	US$166
	Order now
Image	Western Blot Positive WB detected in: Hela whole cell lysate, Raji whole cell lysate, U251 whole cell lysate All lanes: LARS antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 135, 129, 56 kDa Observed band size: 135 kDa IHC image of CSB-PA873736LA01HU diluted at 1:200 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA873736LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Promotion
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) LARS Polyclonal antibody

Uniprot No.

Q9P2J5

Target Names

LARS

Alternative Names

cytoplasmic antibody; Cytoplasmic leucyl tRNA synthetase antibody; Cytosolic leucyl tRNA synthetase antibody; FLJ10595 antibody; FLJ21788 antibody; hr025Cl antibody; HSPC 192 antibody; HSPC192 antibody; KIAA1352 antibody; LARS 1 antibody; Lars antibody; LARS1 antibody; Leucine translase antibody; Leucine tRNA ligase antibody; Leucine--tRNA ligase antibody; Leucyl tRNA synthetase cytoplasmic antibody; Leucyl-tRNA synthetase antibody; LeuRS antibody; LEUS antibody; LRS antibody; PIG 44 antibody; PIG44 antibody; Proliferation inducing gene 44 antibody; RNTLS antibody; SYLC_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Leucine--tRNA ligase, cytoplasmic protein (614-892AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The LARS Antibody (Product code: CSB-PA873736LA01HU) is Non-conjugated. For LARS Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA873736LB01HU	LARS Antibody, HRP conjugated	ELISA
FITC	CSB-PA873736LC01HU	LARS Antibody, FITC conjugated
Biotin	CSB-PA873736LD01HU	LARS Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Exhibits a post-transfer editing activity to hydrolyze mischarged tRNAs.

Gene References into Functions

Leucyl-tRNA synthetase (LRS) is a leucine sensor of the mTORC1 pathway. PMID: 28963468
The results showed a decrease in autophagy on addition of leucine, demonstrating crosstalk between leucine sensing, LRS translocation, RagD interaction, and mTORC1 activation. PMID: 28882589
the KMSKS catalytic loop affects the aminoacylation and editing capacities of leucyl-tRNA synthetase PMID: 25817995
Lack of a CP1 hairpin in LeuRS led to complete loss of aminoacylation, amino acid activation, and tRNA binding; however, the mutants retained post-transfer editing. PMID: 25051973
the carboxy-terminal domain of human mitochondrial (mt) leucyl-tRNA synthetase can be used to correct mt dysfunctions caused by mt-tRNA mutations. PMID: 24413190
Identification of a mutation in LARS as a novel cause of infantile hepatopathy PMID: 22607940
This work demonstrates that LRS is a key mediator for amino acid signaling to mTORC1. PMID: 22424946
hcLeuRS can charge RNALeu with non-cognate amino acids and exclude the incorrect products by multiple editing pathways. PMID: 20805241
leucyl-tRNA synthetase requires its C-terminal domain for its interaction with arginyl-tRNA synthetase in the multi-tRNA synthetase complex PMID: 16055448
We identified a novel G3283A transition in the mitochondrial DNA tRNA(Leu (UUR)) gene in a patient with ptosis, ophthalmoparesis and hyporeflexia. PMID: 17363246
Results show that K600 in human leucyl-tRNA synthetase affects amino acid specificity and tRNA aminoacylation. PMID: 17378584
findings suggest that LARS1 may play roles in migration and growth of lung cancer cells, which suggest its potential implication in lung tumorigenesis PMID: 18446061
Study of crystal structures of the editing domain from 2 eukaryotic cytosolic LeuRS; shows a conserved structural core containing the active site for hydrolysis, with distinct bacterial, archeal, or eukaryotic peripheral insertions. PMID: 19426743
the introduction of bulky residues into the amino acid binding pocket failed to block deacylation of tRNA, indicating that the architecture of the amino acid binding pocket is different compared to that of other characterized LeuRSs PMID: 19702327

Hide All

Involvement in disease

Infantile liver failure syndrome 1 (ILFS1)

Subcellular Location

Cytoplasm.

Protein Families

Class-I aminoacyl-tRNA synthetase family

Database Links

HGNC: 6512

OMIM: 151350

KEGG: hsa:51520

STRING: 9606.ENSP00000377954

UniGene: Hs.432674

Promotions

CUSABIO Premium Active Proteins 20% OFF SALE

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

TNFR1: A Multifaceted Molecule in Cancer, Another Promising Therapeutic Target for COVID-19 How to Validate An Antibody？ GPRC5D: Newly Efficient GPRC5D CAR-T or Bispecific Antibody Therapy to Fight Multiple Myeloma (MM) Biological Activity of Proteins Bladder Cancer: Insights into Molecular Biology,Signaling Pathways, and Biomarkers Growth Hormone - Not Only Promotes Height Increase CD47, A New Target for Tumor Immune CD20 Monoclonal Antibodies Drugs: There is an Explosion of New mAbs Against CD20! The Overview of Hippo Signaling Pathway Latest Changes in Journal Impact Factor IL-17A: A Major Cytokine of the IL-17 Family, a Key Inflammation Target in Skin Diseases, or Inflammation-Associated Cancers! Tumor Necrosis Factor (TNF) A Group of Promising Proteins - Cell Cycle Markers Bispecific Antibody Overview: From Definition to Application CT83/KK-LC-1: a Promising Therapeutic Target of CTAs Family, Currently in Clinical Research for Breast, Lung, Gastric Cancers, and Beyond! What is a His Tag? A Guide from Definition to Applications TNFSF15/TL1A: The Rise of the Next Billion-Dollar Target as Pharma Giants Stake Their Claims How AI is Reshaping Biological Research? A Comprehensive Guide to Tools and Practical Strategies Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address