LDB1 Antibody

Datasheet

Code	CSB-PA771433LA01HU
Size	US$166
	Order now
Image	Western Blot Positive WB detected in: Jurkat whole cell lysate, K562 whole cell lysate All lanes: LDB1 antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 47, 37, 43 kDa Observed band size: 47 kDa Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA771433LA01HU at dilution of 1:100 Immunofluorescent analysis of Hela cells using CSB-PA771433LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Promotion
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) LDB1 Polyclonal antibody

Uniprot No.

Q86U70

Target Names

LDB1

Alternative Names

Carboxyl Terminal LIM Domain Binding 2 antibody; Carboxyl-terminal LIM domain-binding protein 2 antibody; CLIM 2 antibody; CLIM-2 antibody; hLdb1 antibody; LDB-1 antibody; ldb1 antibody; LDB1_HUMAN antibody; LIM Domain Binding 1 antibody; LIM domain binding factor CLIM2 antibody; LIM domain-binding factor CLIM2 antibody; LIM domain-binding protein 1 antibody; NLI antibody; Nuclear LIM Domain Interactor antibody; Nuclear LIM interactor antibody; xldb1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human LIM domain-binding protein 1 protein (50-375AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The LDB1 Antibody (Product code: CSB-PA771433LA01HU) is Non-conjugated. For LDB1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA771433LB01HU	LDB1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA771433LC01HU	LDB1 Antibody, FITC conjugated
Biotin	CSB-PA771433LD01HU	LDB1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. May regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. Plays a role in the development of interneurons and motor neurons in cooperation with LHX3 and ISL1. Acts synergistically with LHX1/LIM1 in axis formation and activation of gene expression. Acts with LMO2 in the regulation of red blood cell development, maintaining erythroid precursors in an immature state.

Gene References into Functions

Data indicate that LIM-domain-binding protein 1 (LDB1) has a strong role in colorectal cancer (CRC) progression. PMID: 27713177
LDB1 maintains the terminally differentiated state of beta cells and is a component of active enhancers in both murine and human islets. PMID: 27941246
SSBP3 Interacts With Islet-1 and Ldb1 to Impact Pancreatic beta-Cell Target Genes PMID: 26495868
Alanine scanning mutagenesis of the LIM interaction domain of LDB1 revealed a discrete motif, R(320)LITR, required for LMO2 binding. PMID: 26598604
In t(8;21) leukemia cells, LDB1 functions as a component of the stable AML1-ETO-containing transcription factor complex (AETFC). The AETFC components cooperatively regulate gene expression and contribute to leukemogenesis. PMID: 23812588
We investigated NLI (Ldb1 homolog) complex occupancy and chromatin conformation of the beta-globin locus in human erythroid cells. PMID: 22010104
These studies are consistent with a model in which TIF1gamma acts to ubiquitinate LDB1 leading to degradation of LDB1 and changes in transcription of LDB1-dependent promoters. PMID: 20447379
Expression of LDB1 (LIM-domain-binding 1) protein in which Lys134 is replaced with arginine leads to enhanced expression of the mutant protein compared with the wild-type protein. PMID: 20423330
Ssdp proteins interact with the LIM-domain-binding protein Ldb1 to regulate development PMID: 12381786
characterize the assembly of a five-component complex containing TAL1, LMO2, Ldb1, E12, and DNA. The bHLH domains of TAL1 and E12 alone primarily formed helical homodimers, but together formed heterodimers, to which LMO2 bound with high affinity PMID: 17910069

Hide All

Subcellular Location

Nucleus.

Protein Families

LDB family

Tissue Specificity

Expressed in a wide range of adult tissues including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.

Database Links

HGNC: 6532

OMIM: 603451

KEGG: hsa:8861

STRING: 9606.ENSP00000392466

UniGene: Hs.454418

Promotions

CUSABIO Premium Active Proteins 20% OFF SALE

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address