LRTM2 Antibody

Datasheet

Code	CSB-PA847660LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: SH-SY5Y whole cell lysate, U251 whole cell lysate, Rat brain tissue All lanes: LRTM2 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa IHC image of CSB-PA847660LA01HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA847660LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Western Blot Positive WB detected in: HepG2 whole cell lysate, SH-SY5Y whole cell lysate, U87 whole cell lysate, U251 whole cell lysate, 293 whole cell lysate All lanes: LRTM2 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) LRTM2 Polyclonal antibody

Uniprot No.

Q8N967

Target Names

LRTM2

Alternative Names

Leucine-rich repeat and transmembrane domain-containing protein 2 antibody; Leucine-rich repeats and transmembrane domains 2 antibody; Lrtm2 antibody; LRTM2_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human, Rat

Immunogen

Recombinant Human Leucine-rich repeat and transmembrane domain-containing protein 2 protein (36-310AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The LRTM2 Antibody (Product code: CSB-PA847660LA01HU) is Non-conjugated. For LRTM2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA847660LB01HU	LRTM2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA847660LC01HU	LRTM2 Antibody, FITC conjugated
Biotin	CSB-PA847660LD01HU	LRTM2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Subcellular Location

Membrane; Single-pass type I membrane protein.

Database Links

HGNC: 32443

KEGG: hsa:654429

STRING: 9606.ENSP00000299194

UniGene: Hs.585579

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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