LRTM2 Antibody

Datasheet
Code CSB-PA847660LA01HU
Size US$299
Uniprot No. Q8N967
Image
  • Western Blot
    Positive WB detected in: SH-SY5Y whole cell lysate, U251 whole cell lysate, Rat brain tissue
    All lanes: LRTM2 antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 42 kDa
    Observed band size: 42 kDa

  • IHC image of CSB-PA847660LA01HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of HepG2 cells with CSB-PA847660LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Western Blot
    Positive WB detected in: HepG2 whole cell lysate, SH-SY5Y whole cell lysate, U87 whole cell lysate, U251 whole cell lysate, 293 whole cell lysate
    All lanes: LRTM2 antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 42 kDa
    Observed band size: 42 kDa

Immunogen Recombinant Human Leucine-rich repeat and transmembrane domain-containing protein 2 protein (36-310AA)
Raised in Rabbit
Species Reactivity Human, Rat
Tested Applications ELISA, WB, IHC, IF; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Relevance cytoplasm, protein kinase inhibitor activity, cytokine-mediated signaling pathway, negative regulation of JAK-STAT cascade, negative regulation of protein kinase activity.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Leucine-rich repeat and transmembrane domain-containing protein 2, LRTM2
Immunogen Species Human
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Research Area Cell Biology
Target Names LRTM2
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Subcellular Location Membrane, Single-pass type I membrane protein
Database Links

HGNC: 32443

KEGG: hsa:654429

STRING: 9606.ENSP00000299194

UniGene: Hs.585579

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