MAEA Antibody

Datasheet

Code	CSB-PA013310LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate All lanes: MAEA antibody at 3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 46, 41, 37, 27 kDa Observed band size: 46 kDa IHC image of CSB-PA013310LA01HU diluted at 1:600 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA013310LA01HU diluted at 1:600 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescent analysis of Hela cells using CSB-PA013310LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) MAEA Polyclonal antibody

Uniprot No.

Q7L5Y9

Target Names

MAEA

Alternative Names

1110030D19Rik antibody; Cell proliferation inducing gene 5 protein antibody; Cell proliferation-inducing gene 5 protein antibody; EMLP antibody; EMP antibody; Erythroblast macrophage protein antibody; GID complex subunit 9 FYV10 homolog antibody; GID9 antibody; HLC 10 antibody; HLC-10 antibody; HLC10 antibody; Human lung cancer oncogene 10 protein antibody; Lung cancer related protein 10 antibody; Macrophage erythroblast attacher antibody; MAEA antibody; MAEA_HUMAN antibody; MGC93683 antibody; PIG5 antibody; Proliferation inducing gene 5 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Macrophage erythroblast attacher protein (299-356AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The MAEA Antibody (Product code: CSB-PA013310LA01HU) is Non-conjugated. For MAEA Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA013310LB01HU	MAEA Antibody, HRP conjugated	ELISA
FITC	CSB-PA013310LC01HU	MAEA Antibody, FITC conjugated
Biotin	CSB-PA013310LD01HU	MAEA Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1. MAEA and RMND5A are both required for catalytic activity of the CTLH E3 ubiquitin-protein ligase complex. MAEA is required for normal cell proliferation. The CTLH E3 ubiquitin-protein ligase complex is not required for the degradation of enzymes involved in gluconeogenesis, such as FBP1. Plays a role in erythroblast enucleation during erythrocyte maturation and in the development of mature macrophages. Mediates the attachment of erythroid cell to mature macrophages; this MAEA-mediated contact inhibits erythroid cell apoptosis. Participates in erythroblastic island formation, which is the functional unit of definitive erythropoiesis. Associates with F-actin to regulate actin distribution in erythroblasts and macrophages. May contribute to nuclear architecture and cells division events (Probable).

Gene References into Functions

Emp expression correlates with erythroblastic island formation and has an important function for bone marrow hematopoiesis. PMID: 23566571
These results suggest that Emp undergoes dynamic rearrangements within the nuclear architecture that are correlated with cell division. PMID: 16510120
RanBPM, ARMC8alpha, ARMC8beta, Muskelin, p48EMLP, and p44CTLH form complexes in cells. PMID: 17467196

Subcellular Location

Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix. Cell membrane. Cytoplasm, cytoskeleton.

Tissue Specificity

Detected at macrophage membranes (at protein level). Ubiquitous.

Database Links

HGNC: 13731

OMIM: 606801

KEGG: hsa:10296

STRING: 9606.ENSP00000302830

UniGene: Hs.139896

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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