MEGF8 Antibody

Datasheet

Code	CSB-PA773606LA01HU
Size	US$166
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Image	IHC image of CSB-PA773606LA01HU diluted at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA773606LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) MEGF8 Polyclonal antibody

Uniprot No.

Q7Z7M0

Target Names

MEGF8

Alternative Names

MEGF8 antibody; C19orf49 antibody; EGFL4 antibody; KIAA0817 antibody; Multiple epidermal growth factor-like domains protein 8 antibody; Multiple EGF-like domains protein 8 antibody; Epidermal growth factor-like protein 4 antibody; EGF-like protein 4 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Multiple epidermal growth factor-like domains protein 8 protein (495-663AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The MEGF8 Antibody (Product code: CSB-PA773606LA01HU) is Non-conjugated. For MEGF8 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA773606LB01HU	MEGF8 Antibody, HRP conjugated	ELISA
FITC	CSB-PA773606LC01HU	MEGF8 Antibody, FITC conjugated
Biotin	CSB-PA773606LD01HU	MEGF8 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Acts as a negative regulator of hedgehog signaling.

Gene References into Functions

mutations in MEGF8 cause a Carpenter syndrome subtype frequently associated with defective left-right patterning, probably through perturbation of signaling by hedgehog and nodal family members. PMID: 23063620

Involvement in disease

Carpenter syndrome 2 (CRPT2)

Subcellular Location

Membrane; Single-pass type I membrane protein.

Database Links

HGNC: 3233

OMIM: 604267

KEGG: hsa:1954

STRING: 9606.ENSP00000334219

UniGene: Hs.132483

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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