MFSD2A Antibody

Datasheet

Code	CSB-PA196650
Size	US$166
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Image	The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA196650 (MFSD2A Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200) Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Human placenta tissue lysate, Primary antibody: CSB-PA196650 (MFSD2A Antibody) at dilution 1/500, Secondary antibody: D110058(HRP-conjugated Goat anti rabbit IgG) at 1/5000 dilution, Exposure time: 1 minute
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Product Details
Citations
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

Q8NA29

Target Names

MFSD2A

Alternative Names

1700018O18Rik antibody; FLJ14490 antibody; FLJ35904 antibody; Major facilitator superfamily domain containing 2 antibody; Major facilitator superfamily domain containing 2A antibody; Major facilitator superfamily domain-containing protein 2A antibody; MFS2A_HUMAN antibody; MFSD2 antibody; MFSD2A antibody; RGD1310174 antibody; RP23-121J14.3 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Synthetic peptide of Human MFSD2A

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
ELISA	1:1000-1:2000
WB	1:500-1:2000
IHC	1:25-1:100

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

Brain endothelial PTEN/AKT/NEDD4-2/MFSD2A axis regulates blood-brain barrier permeability Y Cui,Cell Reports,2021

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

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Applications : Western blot assays

Sample type: Human cells

Review: Western blot assays of the MFSD2A, p-AKT1, AKT1, p-NEDD4-2(S342/S448), NEDD4-2, and GAPDH levels in MFSD2A-expressing HEK293T cells treated with IGF-1 and siRNAs targeting NEDD4-2. siNEDD4-2 treatment increased the MFSD2A levels and blocked the decrease in MFSD2A protein induced by IGF-1.

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Target Background

Function

Sodium-dependent lysophosphatidylcholine (LPC) symporter, which plays an essential role for blood-brain barrier formation and function. Specifically expressed in endothelium of the blood-brain barrier of micro-vessels and transports LPC into the brain. Transport of LPC is essential because it constitutes the major mechanism by which docosahexaenoic acid (DHA), an omega-3 fatty acid that is essential for normal brain growth and cognitive function, enters the brain. Transports LPC carrying long-chain fatty acids such LPC oleate and LPC palmitate with a minimum acyl chain length of 14 carbons. Does not transport docosahexaenoic acid in unesterified fatty acid. Specifically required for blood-brain barrier formation and function, probably by mediating lipid transport. Not required for central nervous system vascular morphogenesis. Acts as a transporter for tunicamycin, an inhibitor of asparagine-linked glycosylation. In placenta, acts as a receptor for ERVFRD-1/syncytin-2 and is required for trophoblast fusion.

Gene References into Functions

In offspring of women with gestational diabetes mellitus treated either with diet or insulin, higher fetal fat accretion and lower placental MFSD2a contribute to reduce docosahexaenoic acid availability. PMID: 26869380
Levels of DHA-derived epoxides are lower in colon tissues from patients with ulcerative colitis than healthy and resolving mucosa. Production of these metabolites by gut endothelium requires MFSD2A; endothelial progenitor cells that overexpress MFSD2A reduce colitis in mice. PMID: 28827082
The regulatory role of Mfsd2a deepens our knowledge of the function of the BBB, potentially contributing to the effective drug delivery in the treatments for neurodegenerative diseases, brain tumors, and life-threatening infections in the CNS PMID: 26747400
MFSD2A transported structurally related acylcarnitines but not a lysolipid without a negative charge, demonstrating the necessity of a negatively charged headgroup interaction with Lys-436 for transport. PMID: 26945070
A homozygous mutation affecting a highly conserved MFSD2A residue (p.Ser339Leu) is associated with a progressive microcephaly syndrome characterized by intellectual disability, spasticity and absent speech. PMID: 26005865
MFSD2A mutations impair brain lipid transport activity. PMID: 26005868
Several tagging SNPs and haplotypes in TRIT1, MYCL1 and MFSD2A region are significantly associated with risk and clinicopathological features of gastric cancer in a Chinese population. PMID: 23349019
Importance of MFSD2a in trophoblast fusion and placenta development. PMID: 23177091
SNP rs12072037 modulates MFSD2A promoter activity and thus might affect MFSD2A levels in normal lung and in lung tumors. PMID: 21736709
MFSD2A is a putative Tunicamycin transporter at the plasma membrane. PMID: 21677192
MFSD2A is a novel lung cancer tumor suppressor gene that regulates cell cycle progression and matrix attachment. PMID: 20236515
three additional SNPs in the MFSD2 genes showed ethnic differences in allelic frequencies PMID: 17145094
Results identify Mfsd2a (major facilitator superfamily domain-containing protein 2a) and an additional closely related protein Mfsd2b, and suggest that Mfsd2a plays a role in adaptive thermogenesis. PMID: 18694395
MFSD2 is a placenta-specific receptor for the fusogenic, endogenous retrovirus-derived, human syncytin-2, and plays a role in placenta morphogenesis. PMID: 18988732

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Involvement in disease

Microcephaly 15, primary, autosomal recessive (MCPH15)

Subcellular Location

Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.

Protein Families

Major facilitator superfamily

Tissue Specificity

In placenta, associated with trophoblast cells.

Database Links

HGNC: 25897

OMIM: 614397

KEGG: hsa:84879

STRING: 9606.ENSP00000361895

UniGene: Hs.655177

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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