MICAL1 Antibody

Datasheet

Code	CSB-PA851555LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Raji whole cell lysate, Jurkat whole cell lysate, THP-1 whole cell lysate All lanes: MICAL1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 118, 109, 38, 120 kDa Observed band size: 120 kDa IHC image of CSB-PA851555LA01HU diluted at 1:200 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA851555LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) MICAL1 Polyclonal antibody

Uniprot No.

Q8TDZ2

Target Names

MICAL1

Alternative Names

CasL interacting molecule antibody; DKFZp434B1517 antibody; FLJ11937 antibody; FLJ21739 antibody; MICA1_HUMAN antibody; MICAL 1 antibody; MICAL antibody; MICAL1 antibody; microtubule associated monoxygenase; calponin and LIM domain containing 1 antibody; Molecule interacting with CasL protein 1 antibody; NEDD9 interacting protein with calponin homology and LIM domains antibody; NEDD9-interacting protein with calponin homology and LIM domains antibody; NICAL antibody; OTTHUMP00000016969 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human [F-actin]-methionine sulfoxide oxidase MICAL1 protein (806-1067AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The MICAL1 Antibody (Product code: CSB-PA851555LA01HU) is Non-conjugated. For MICAL1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA851555LB01HU	MICAL1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA851555LC01HU	MICAL1 Antibody, FITC conjugated
Biotin	CSB-PA851555LD01HU	MICAL1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin to form methionine-sulfoxide, resulting in actin filament disassembly and preventing repolymerization. In the absence of actin, it also functions as a NADPH oxidase producing H(2)O(2). Acts as a cytoskeletal regulator that connects NEDD9 to intermediate filaments. Also acts as a negative regulator of apoptosis via its interaction with STK38 and STK38L; acts by antagonizing STK38 and STK38L activation by MST1/STK4. Involved in regulation of lamina-specific connectivity in the nervous system such as the development of lamina-restricted hippocampal connections. Through redox regulation of the actin cytoskeleton controls the intracellular distribution of secretory vesicles containing L1/neurofascin/NgCAM family proteins in neurons, thereby regulating their cell surface levels. May act as Rab effector protein and play a role in vesicle trafficking.

Gene References into Functions

Methionine oxidation is regulated in vivo by monooxygenases of the MICAL family. (Review) PMID: 28229915
describe methods to characterize MICAL-mediated F-actin disassembly using in vitro assays with purified proteins PMID: 27787846
The results provide evidence that MICAL1 plays an essential role in the activation of ROS/Akt signaling and cell invasive phenotype and identify a novel link between RAB35 and MICAL1 in regulating breast cancer cell invasion. PMID: 27430308
The MICAL1 directly induce oxidation of actin molecules, leading to actin depolymerization. ROS production by MICAL1 also causes oxidation of collapsin response mediator protein-2 which subsequently undergoes phosphorylation. PMID: 23834433
although MICAL1 is auto-inhibited by its C-terminal coiled-coil region, MICAL2 remains constitutively active and affects stress fibers, suggesting differential but complementary roles for MICAL1 and MICAL2 in actin microfilament regulation PMID: 22331357
Faint and scattered immunoreactivity for Mical-1 is observed in neurons of the neocortex of the temporal lobe epilepsy group, while strong immunoreactivity for Mical-1 is shown in control subjects. PMID: 21638339
Findings show a biological role for MICAL-1 in apoptosis and define a novel negative regulatory mechanism of MST-NDR signaling. PMID: 21730291
a CasL interacting molecule that associates with vimentin PMID: 11827972
MICAL-1 isoforms with their multidomain structure are novel rab1 interacting proteins that function as scaffold proteins connecting different components in the cell. PMID: 12788069
The unfolding of MICAL-1 calpolnin homology (CH) domain was studied. PMID: 17662518

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Subcellular Location

Cytoplasm. Cytoplasm, cytoskeleton. Midbody.

Protein Families

Mical family

Tissue Specificity

Expressed in the thymus, lung, spleen, kidney, testis and hematopoietic cells.

Database Links

HGNC: 20619

OMIM: 607129

KEGG: hsa:64780

STRING: 9606.ENSP00000351664

UniGene: Hs.33476

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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