MSH4 Antibody

Datasheet

Code	CSB-PA015030LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Mouse lung tissue, Mouse kidney tissue, Human placenta tissue All lanes: MSH4 antibody at 3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 105 kDa Observed band size: 105 kDa Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA015030LA01HU at dilution of 1:100 Immunofluorescence staining of Hela cell with CSB-PA015030LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) MSH4 Polyclonal antibody

Uniprot No.

O15457

Target Names

MSH4

Alternative Names

hMSH4 antibody; MSH 4 antibody; MSH4 antibody; MSH4_HUMAN antibody; MutS homolog 4 (E. Coli) antibody; MutS homolog 4 antibody; MutS protein homolog 4 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human MutS protein homolog 4 protein (501-800AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The MSH4 Antibody (Product code: CSB-PA015030LA01HU) is Non-conjugated. For MSH4 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA015030LB01HU	MSH4 Antibody, HRP conjugated	ELISA
FITC	CSB-PA015030LC01HU	MSH4 Antibody, FITC conjugated
Biotin	CSB-PA015030LD01HU	MSH4 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Involved in meiotic recombination. Required for reciprocal recombination and proper segregation of homologous chromosomes at meiosis.

Gene References into Functions

Study describes the first MSH4 mutation associated with POI and increases the number of meiotic/DNA repair genes formally implicated as being responsible for this condition. PMID: 28541421
inactivation of MSH4 in germ cells may have played a role in the acquisition of additional TP53 and LATS1 germline mutations in a Li-Fraumeni family PMID: 25041856
hMSH4 interacts with HDAC3. PMID: 24145748
Our current study has revealed a role for hMSH4 in the maintenance of genomic stability by suppressing NHEJ-mediated DSB repair. PMID: 23725059
mutation analysis of SYCP3, DNMT3L and MSH4 in patients with maturation arrest of spermatogenesis and couples with recurrent miscarriages PMID: 21126912
The different properties associated with these two hMSH5 variants underscore the potential functional diversity of the human hMSH5 gene. PMID: 15907804
the GPS2 might function in concert with hMSH4-hMSH5 during the process of homologous recombination. PMID: 16122992
A review of the properties and functional roles of MSH4. PMID: 17127347
Dimerization of MSH4 and MSH5 facilitates their nuclear localization suggesting that dimerization may regulate the intracellular trafficking of these proteins. PMID: 17869244
Data have identified the interaction region between the individual subunits of hMSH4-hMSH5 that are likely involved in clamp formation and show that each subunit of the heterodimer binds ATP. PMID: 17977839
Data show that there is no association between MSH4 gene mutation and premature ovarian failure. PMID: 18166824

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Subcellular Location

Chromosome.

Protein Families

DNA mismatch repair MutS family

Tissue Specificity

Testis and ovary specific.

Database Links

HGNC: 7327

OMIM: 602105

KEGG: hsa:4438

STRING: 9606.ENSP00000263187

UniGene: Hs.216639

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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