MTRF1 Antibody

Datasheet

Code	CSB-PA015197LA01HU
Size	US$166
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Image	Immunofluorescence staining of Hela cells with CSB-PA015197LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA015197LA01HU at dilution of 1:100 Western Blot Positive WB detected in: Hela whole cell lysate All lanes: MTRF1 antibody at 3μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 53, 55 kDa Observed band size: 53 kDa
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) MTRF1 Polyclonal antibody

Uniprot No.

O75570

Target Names

MTRF1

Alternative Names

mitochondrial antibody; MRF-1 antibody; MRF1 antibody; MtRF-1 antibody; MTRF1 antibody; Peptide chain release factor 1 antibody; Peptide chain release factor 1; mitochondrial antibody; RF1M_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Peptide chain release factor 1, mitochondrial protein (285-433AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The MTRF1 Antibody (Product code: CSB-PA015197LA01HU) is Non-conjugated. For MTRF1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA015197LB01HU	MTRF1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA015197LC01HU	MTRF1 Antibody, FITC conjugated
Biotin	CSB-PA015197LD01HU	MTRF1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Mitochondrial peptide chain release factor that directs the termination of translation in response to the peptide chain non-cognate termination stop codons AGG and AGA.

Gene References into Functions

Purified mtRF1 did not induce translation termination in vitro nor did mtRF1 restore mitochondrial translation machinery in mutated S. cerevisiae in vivo. A different mtRF, mtRF1a, was found. PMID: 17803939

Subcellular Location

Mitochondrion.

Protein Families

Prokaryotic/mitochondrial release factor family

Database Links

HGNC: 7469

OMIM: 604601

KEGG: hsa:9617

STRING: 9606.ENSP00000368790

UniGene: Hs.382176

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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