Mono-methyl-HIST1H1C (K45) Antibody

Datasheet
Code CSB-PA010378OA45me1HU
Size US$319
Image
  • Western Blot
    Positive WB detected in: 293 whole cell lysate, A549 whole cell lysate
    All lanes: HIST1H1C antibody at 1:100
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 22 kDa
    Observed band size: 22 kDa

  • Immunocytochemistry analysis of CSB-PA010378OA45me1HU diluted at 1:25 and staining in Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of Hela cells with CSB-PA010378OA45me1HU at 1:12.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Product Details

Full Product Name Rabbit anti-Human HIST1H1C Polyclonal antibody
Uniprot No. P16403
Target Names HIST1H1C
Alternative Names Histone H1.2 (Histone H1c) (Histone H1d) (Histone H1s-1), HIST1H1C, H1F2
Raised in Rabbit
Species Reactivity Human
Immunogen Peptide sequence around site of Mono-methyl-Lys (45) derived from Human Histone H1.2
Immunogen Species Human
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Antigen Affinity Purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, ICC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:100-1:1000
ICC 1:20-1:200
IF 1:10-1:100
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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Target Data

Function Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Subcellular Location Nucleus, Chromosome
Protein Families Histone H1/H5 family
Database Links

HGNC: 4716

OMIM: 142710

KEGG: hsa:3006

STRING: 9606.ENSP00000339566

UniGene: Hs.7644

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