NAGPA Antibody

Datasheet

Code	CSB-PA015417LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: SH-SY5Y whole cell lysate, PC-3 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate All lanes: NAGPA antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57, 53, 34 kDa Observed band size: 53 kDa IHC image of CSB-PA015417LA01HU diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) NAGPA Polyclonal antibody

Uniprot No.

Q9UK23

Target Names

NAGPA

Alternative Names

Mannose 6-phosphate-uncovering enzyme antibody; N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase antibody; NAGPA antibody; NAGPA_HUMAN antibody; Phosphodiester alpha-GlcNAcase antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase protein (327-438AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The NAGPA Antibody (Product code: CSB-PA015417LA01HU) is Non-conjugated. For NAGPA Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA015417LB01HU	NAGPA Antibody, HRP conjugated	ELISA
FITC	CSB-PA015417LC01HU	NAGPA Antibody, FITC conjugated
Biotin	CSB-PA015417LD01HU	NAGPA Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Catalyzes the second step in the formation of the mannose 6-phosphate targeting signal on lysosomal enzyme oligosaccharides by removing GlcNAc residues from GlcNAc-alpha-P-mannose moieties, which are formed in the first step. Also hydrolyzes UDP-GlcNAc, a sugar donor for Golgi N-acetylglucosaminyltransferases.

Gene References into Functions

14 variations were found in GNPTAB, GNPTG and NAGPA genes. PMID: 29289611
SNPs covering GNPTAB, GNPTG and NAGPA were subjected to genotyping, association analysis was performed on all SNPs. Significant association of rs17031962 in GNPTAB and rs882294 in NAGPA with developmental dyslexia in a Chinese population was identified after false discovery rate correction for multiple comparisons. PMID: 25643770
Mutational analysis of several residues in a highly conserved surface cavity of hUCE revealed that they are essential for function. PMID: 23572527
To date mutations in GNPTAB, GNPTG, and NAGPA have been associated with stuttering. These genes encode the lysosomal enzyme targeting pathway, defective in mucolipidosis. (Review) PMID: 22884963
Analysis of mannose 6-phosphate uncovering enzyme mutations associated with persistent stuttering. PMID: 21956109
identified three mutations in the NAGPA gene associated with stuttering PMID: 20147709
synthesis as a proenzyme that is activated by furin [mannose 6-phosphate-uncovering enzyme] PMID: 12058031
The mannose 6-phosphate uncovering enzyme participates in the uncovering of the mannose 6-phosphate recognition tag on lysosomal enzymes, a process that facilitates recognition of those enzymes by mannose 6-phosphate receptors to delivery to lysosomes. PMID: 15976452

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Involvement in disease

Defects in NAGPA have been suggested to play a role in susceptibility to persistent stuttering. Stuttering is a common speech disorder characterized by repetitions, prolongations, and interruptions in the flow of speech.

Subcellular Location

Golgi apparatus, Golgi stack membrane; Single-pass type I membrane protein. Golgi apparatus, trans-Golgi network. Note=Cis/medial Golgi.

Tissue Specificity

Isoform 2 may be brain-specific.

Database Links

HGNC: 17378

OMIM: 607985

KEGG: hsa:51172

STRING: 9606.ENSP00000310998

UniGene: Hs.21334

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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