NANOG Antibody

Datasheet
Code CSB-PA888008LA01HU
See More Details Free Antibody trial simple
Size US$299Purchase it in Cusabio online store
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Uniprot No. Q9H9S0
Image
  • Western Blot
    Positive WB detected in: Ntera-2 whole cell lysate
    All lanes: NANOG antibody at 3µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 35, 33 kDa
    Observed band size: 35 kDa

  • Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA888008LA01HU at dilution of 1:100

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunogen Recombinant Human Homeobox protein NANOG protein (1-305AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC; Recommended dilution: WB:1:2000-1:5000, IHC:1:20-1:200
Relevance Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Homeobox protein NANOG (Homeobox transcription factor Nanog) (hNanog), NANOG
Immunogen Species Human
Research Area Stem Cells
Target Names NANOG
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter
Subcellular Location Nucleus
Protein Families Nanog homeobox family
Tissue Specificity Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and
Database Links

HGNC: 20857

OMIM: 607937

KEGG: hsa:79923

STRING: 9606.ENSP00000229307

UniGene: Hs.635882

Pathway Signaling pathways regulating pluripotency of stem cells

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