NANOG

The NANOG homeobox protein (NANOG) is a core pluripotency transcription factor, with aliases including NANOG1 (to distinguish from its pseudogene paralogs). It sustains self-renewal of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) by forming a regulatory circuit with OCT4 and SOX2—binding to their promoters and those of other stemness-associated genes, while repressing transcription of lineage-specific differentiation markers.
Aberrant NANOG overexpression is implicated in cancer stem cell (CSC) maintenance across malignancies like breast, lung, and glioblastoma, driving tumor initiation, metastasis, and chemoresistance. It intersects with key signaling pathways (Wnt/β-catenin, Notch, TGF-β) to modulate CSC properties. Preclinical research focuses on small-molecule inhibitors targeting NANOG’s DNA-binding activity or downstream effectors, which hold promise for eradicating CSCs and improving cancer treatment outcomes.

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

NANOG Recombinant Monoclonal Antibody (CSB-RA032801A0HU)

Validated Data

Overlay Peak curve showing Hela cells stained with CSB-RA032801A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

NANOG Antibody (CSB-PA888008LA01HU)

Validated Data

Western Blot
Positive WB detected in: Ntera-2 whole cell lysate
All lanes: NANOG antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 35, 33 kDa
Observed band size: 35 kDa

Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA888008LA01HU at dilution of 1:100

The following NANOG reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

NANOG Antibodies

NANOG Proteins

NANOG Antibodies

NANOG Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA015424GA01HU	NANOG Antibody	Human	ELISA,WB,IF
CSB-PA888008LA01HU	NANOG Antibody	Human	ELISA, WB, IHC
CSB-PA888008LC01HU	NANOG Antibody, FITC conjugated	Human
CSB-PA888008LB01HU	NANOG Antibody, HRP conjugated	Human	ELISA
CSB-PA888008LD01HU	NANOG Antibody, Biotin conjugated	Human	ELISA
CSB-MA888008A0m	NANOG Monoclonal Antibody	Human, Mouse, Rat	ELISA, WB, ICC, IF, FC
CSB-RA032801A0HU	NANOG Recombinant Monoclonal Antibody	Human	ELISA, FC
CSB-RA888008MA1HU	NANOG Recombinant Monoclonal Antibody	Human, Mouse	ELISA, WB, FC

NANOG Proteins

NANOG Proteins for Pan troglodytes (Chimpanzee)

Code	Product Name	Source
CSB-YP663393EQV CSB-EP663393EQV CSB-BP663393EQV CSB-MP663393EQV CSB-EP663393EQV-B	Recombinant Pan troglodytes Homeobox protein NANOG (NANOG)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

NANOG Proteins for Bos taurus (Bovine)

Code	Product Name	Source
CSB-YP678046BO CSB-EP678046BO CSB-BP678046BO CSB-MP678046BO CSB-EP678046BO-B	Recombinant Bovine Homeobox protein NANOG (NANOG)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

NANOG Proteins for Mus musculus molossinus (Japanese house mouse)

Code	Product Name	Source
CSB-YP722589MUX CSB-EP722589MUX CSB-BP722589MUX CSB-MP722589MUX CSB-EP722589MUX-B	Recombinant Mus musculus molossinus Homeobox protein NANOG (Nanog)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

NANOG Proteins for Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Code	Product Name	Source
CSB-YP725910MOV CSB-EP725910MOV CSB-BP725910MOV CSB-MP725910MOV CSB-EP725910MOV-B	Recombinant Macaca fascicularis Homeobox protein NANOG (NANOG)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

NANOG Proteins for Mus musculus (Mouse)

Code	Product Name	Source
CSB-YP800371MO CSB-EP800371MO CSB-BP800371MO CSB-MP800371MO CSB-EP800371MO-B	Recombinant Mouse Homeobox protein NANOG (Nanog)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

NANOG Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP888008HU	Recombinant Human Homeobox protein NANOG (NANOG)	Yeast
CSB-EP888008HU	Recombinant Human Homeobox protein NANOG (NANOG)	E.coli
CSB-BP888008HU CSB-MP888008HU CSB-EP888008HU-B	Recombinant Human Homeobox protein NANOG (NANOG)	Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-EP888008HUb1	Recombinant Human Homeobox protein NANOG (NANOG)	E.coli

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NANOG

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