NCAM2 Antibody

Datasheet

Code	CSB-PA015512LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: NCAM2 antibody at 2.7μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 94, 48 kDa Observed band size: 94 kDa Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA015512LA01HU at dilution of 1:100 Immunofluorescence staining of HepG2 cells with CSB-PA015512LA01HU at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) NCAM2 Polyclonal antibody

Uniprot No.

O15394

Target Names

NCAM2

Alternative Names

Cell adhesion molecule, neural, 2 antibody; MGC51008 antibody; N CAM 2 antibody; N CAM 21 antibody; N CAM2 antibody; N CAM21 antibody; N-CAM-2 antibody; NCAM 2 antibody; NCAM 21 antibody; NCAM-2 antibody; Ncam2 antibody; NCAM2_HUMAN antibody; NCAM21 antibody; Neural cell adhesion molecule 2 antibody; OCAM GPI antibody; R4B12 antibody; RB 8 neural cell adhesion molecule antibody; RB8 neural cell adhesion molecule antibody; RNCAM antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Neural cell adhesion molecule 2 protein (209-333AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The NCAM2 Antibody (Product code: CSB-PA015512LA01HU) is Non-conjugated. For NCAM2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA015512LB01HU	NCAM2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA015512LC01HU	NCAM2 Antibody, FITC conjugated
Biotin	CSB-PA015512LD01HU	NCAM2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

May play important roles in selective fasciculation and zone-to-zone projection of the primary olfactory axons.

Gene References into Functions

Our reported case raises the questions whether the NCAM2-deletion is the true cause of the autism spectrum disorder or only a risk factor and whether there might be any connection in NCAM2 with skull-size PMID: 27596683
Abeta-dependent disruption of NCAM2 functions in Alzheimer's disease hippocampus contributes to synapse loss. PMID: 26611261
A complete structural model of the entire ectodomain of human NCAM2 has been assembled from crystal structures of six recombinant proteins corresponding to different regions of the ectodomain. PMID: 21300289
High NCAM2 is associated with increased 5-Fluorouracil sensitivity in prostate and breast cancer. PMID: 21214674
In the crystal structure, two Ig domains interact by domain swapping, as the two N-terminal beta-strands are interchanged. PMID: 18706912

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Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Tissue Specificity

Expressed most strongly in adult and fetal brain.

Database Links

HGNC: 7657

OMIM: 602040

KEGG: hsa:4685

STRING: 9606.ENSP00000383392

UniGene: Hs.473450

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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