NCEH1 Antibody

Datasheet

Code	CSB-PA063661
Size	US$166
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Image	The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA063661(NCEH1 Antibody) at dilution 1/80, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using CSB-PA063661(NCEH1 Antibody) at dilution 1/80, on the right is treated with fusion protein. (Original magnification: ×200) Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Mouse kidney tissue, Mouse heart tissue, Primary antibody: CSB-PA063661(NCEH1 Antibody) at dilution 1/1350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds
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Product Details
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Target Background

Product Details

Uniprot No.

Q6PIU2

Target Names

NCEH1

Alternative Names

Arylacetamide deacetylase like 1 antibody; Arylacetamide deacetylase-like 1 antibody; NCEH antibody; Nceh1 antibody; NCEH1_HUMAN antibody; Neutral cholesterol ester hydrolase 1 antibody; Neutral cholesterol ester hydrolase antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Fusion protein of Human NCEH1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Form

Liquid

Tested Applications

ELISA,WB,IHC

Recommended Dilution

Application	Recommended Dilution
ELISA	1:2000-1:10000
WB	1:1000-1:5000
IHC	1:100-1:300

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Hydrolyzes 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. May be responsible for cholesterol ester hydrolysis in macrophages. Also involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds. May contribute to cancer pathogenesis by promoting tumor cell migration.

Gene References into Functions

Authors discovered that the presence of cholesterol, LDLR-mediated cholesterol endocytosis, and cholesterol efflux are all essential to NCEH-1-mediated neuroprotection. In protecting from alpha-synuclein neurotoxicity, NCEH-1 also stimulates cholesterol-derived neurosteroid formation and lowers cellular reactive oxygen species in mitochondria. PMID: 28934392
Psoriasis inflammation induced microRNA targets NCEH1 in underlying subcutaneous adipose tissue. PMID: 27015452
Together, the results define the kinetics of inhibition of KIAA1363 by active metabolites of agrochemicals and indicate that KIAA1363 is highly sensitive to inhibition by these compounds. PMID: 26617293
Pioglitazone increases ABCA1 expression in an LXR-dependent manner and NCEH1 expression in an LXRalpha-independent manner. PMID: 25280398
Inhibition of AADACL1 activity with a variety of agents blocked platelet aggregation in response to multiple agonists; blocked activation of the small GTPase RAP1 and protein kinase C (PKC). PMID: 23993462
NCEH1 is expressed in human atheromatous lesions, where it plays a critical role in the hydrolysis of cholesterol ester in human macrophage foam cells, thereby contributing to the initial part of reverse cholesterol transport in human atherosclerosis. PMID: 20947831
Ritonavir (at 100 mg once daily and 100 mg twice daily significantly down-regulated neutral cholesterol ester hydrolase 1 in 20 healthy individuals. PMID: 20353815
KIAA1363, an uncharacterized enzyme highly elevated in aggressive cancer cells, serves as a central node in an ether lipid signaling network that bridges platelet-activating factor and lysophosphatidic acid. PMID: 17052604
The KIAA1363, an uncharacterized enzyme highly elevated in aggressive cancer cells, serves as a central node in an ether lipid signaling network that bridges platelet-activating factor and lysophosphatidic acid. PMID: 17052608
NCEH is responsible for a major part of nCEH activity in macrophages and may be a potential therapeutic target for the prevention of atherosclerosis. PMID: 18782767

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Subcellular Location

Cell membrane; Single-pass type II membrane protein. Microsome.

Protein Families

'GDXG' lipolytic enzyme family

Tissue Specificity

Expressed in monocyte-derived macrophages. Up-regulated in invasive melanoma and breast carcinoma cell lines.

Database Links

HGNC: 29260

OMIM: 613234

KEGG: hsa:57552

STRING: 9606.ENSP00000442464

UniGene: Hs.444099

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Protein Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Cholesterol metabolism

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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