NDUFAF4 Antibody

Datasheet

Code	CSB-PA959809
Size	US$166
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Image	The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using CSB-PA959809(NDUFAF4 Antibody) at dilution 1/45, on the right is treated with synthetic peptide. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using CSB-PA959809(NDUFAF4 Antibody) at dilution 1/45, on the right is treated with synthetic peptide. (Original magnification: ×200) Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane: Raji cells, Primary antibody: CSB-PA959809(NDUFAF4 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

Q9P032

Target Names

NDUFAF4

Alternative Names

C6orf66 antibody; Hormone-regulated proliferation-associated protein of 20 kDa antibody; HRPAP20 antibody; HSPC125 antibody; My013 antibody; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4 antibody; NDUF4_HUMAN antibody; Ndufaf4 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Synthetic peptide of Human NDUFAF4

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Form

Liquid

Tested Applications

ELISA,WB,IHC

Recommended Dilution

Application	Recommended Dilution
ELISA	1:2000-1:5000
WB	1:500-1:2000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Involved in the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I). May be involved in cell proliferation and survival of hormone-dependent tumor cells. May be a regulator of breast tumor cell invasion.

Gene References into Functions

A homozygous missense NDUFAF4 variant was identified in a complex I-deficient patient with Leigh syndrome. PMID: 28853723
observations suggest that HRPAP20 may be an important regulator of breast tumor cell invasion by a CaM-mediated mechanism that leads to increased MMP-9 secretion PMID: 17001319
HRPAP20 and TIMELESS as promising markers of tamoxifen resistance in women with ER alpha-positive breast tumors. PMID: 17909269
Homozygosity mapping of 5 patients from a consanguineous family with infantile mitochondrial encephalomyopathy resulted in the identification of a missense mutation in a conserved residue of the C6ORF66. PMID: 18179882
Specific lack of complex I was detected in thyroid cancers expressing less than 5% of the amount in surrounding non-cancerous tissue. PMID: 19352385

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Involvement in disease

Mitochondrial complex I deficiency (MT-C1D)

Subcellular Location

Mitochondrion. Membrane; Lipid-anchor.

Protein Families

NDUFAF4 family

Database Links

HGNC: 21034

OMIM: 252010

KEGG: hsa:29078

STRING: 9606.ENSP00000358272

UniGene: Hs.512144

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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