NDUFS2 Antibody

Datasheet

Code	CSB-PA015661LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Rat kidney tissue, Mouse heart tissue, Mouse brain tissue, Mouse kidney tissue All lanes: NDUFS2 antibody at 6.9μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 53, 52 kDa Observed band size: 53 kDa IHC image of CSB-PA015661LA01HU diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA015661LA01HU diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) NDUFS2 Polyclonal antibody

Uniprot No.

O75306

Target Names

NDUFS2

Alternative Names

CI 49 antibody; CI 49kD antibody; CI-49kD antibody; Complex 1, mitochondrial respiratory chain, 49 KD subunit antibody; Complex I 49kD antibody; Complex I 49kDa subunit antibody; Complex I-49kD antibody; mitochondrial antibody; NADH dehydrogenase (ubiquinone) Fe S protein 2 49kDa antibody; NADH dehydrogenase (ubiquinone) Fe S protein 2, 49kDa (NADH coenzyme Q reductase) antibody; NADH dehydrogenase [ubiquinone] iron sulfur protein 2, mitochondrial antibody; NADH dehydrogenase [ubiquinone] iron-sulfur protein 2 antibody; NADH ubiquinone oxidoreductase 49 kDa subunit antibody; NADH ubiquinone oxidoreductase NDUFS2 subunit antibody; NADH-ubiquinone oxidoreductase 49 kDa subunit antibody; NADH:ubiquinone oxidoreductase core subunit S2 antibody; Ndufs2 antibody; NDUS2_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human, Rat, Mouse

Immunogen

Recombinant Human NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial protein (297-427AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The NDUFS2 Antibody (Product code: CSB-PA015661LA01HU) is Non-conjugated. For NDUFS2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA015661LB01HU	NDUFS2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA015661LC01HU	NDUFS2 Antibody, FITC conjugated
Biotin	CSB-PA015661LD01HU	NDUFS2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity of complex I. Essential for the assembly of complex I. Redox-sensitive, critical component of the oxygen-sensing pathway in the pulmonary vasculature which plays a key role in acute pulmonary oxygen-sensing and hypoxic pulmonary vasoconstriction. Plays an important role in carotid body sensing of hypoxia. Essential for glia-like neural stem and progenitor cell proliferation, differentiation and subsequent oligodendrocyte or neuronal maturation.

Gene References into Functions

compound heterozygosity for severe and hypomorphic NDUFS2 mutations can cause non-syndromic hereditary optic neuropathy. PMID: 28031252
NDUFAF7 methylates arginine 85 in the NDUFS2 subunit of human complex I. PMID: 24089531
study describes clinical, radiological, biochemical and molecular data of 6 patients with Leigh syndrome with novel mutations in NDUFV1 and NDUFS2; 2 siblings were compound heterozygotes for an undescribed E104A mutation in NDUFS2 PMID: 23266820
The NDUFS2 mutation affects complex I enzymatic function. PMID: 22036843
Our results confirm that NDUFS2 is a mutational hotspot in Caucasian children with isolated complex I deficiency and recommend the routine diagnostic investigation of this gene in patients with Leigh or Leigh-like phenotypes. PMID: 20819849

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Involvement in disease

Mitochondrial complex I deficiency (MT-C1D)

Subcellular Location

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.

Protein Families

Complex I 49 kDa subunit family

Database Links

HGNC: 7708

OMIM: 252010

KEGG: hsa:4720

STRING: 9606.ENSP00000356972

UniGene: Hs.173611

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Oxidative Phosphorylation

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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