Product Details

Uniprot No.

P51955

Target Names

NEK2

Alternative Names

HSPK 21 antibody; HsPK21 antibody; NEK 2 antibody; NEK2 antibody; NEK2_HUMAN antibody; NEK2A antibody; Never in mitosis A-related kinase 2 antibody; NIMA (never in mitosis gene a) related kinase 2 antibody; NimA like protein kinase 1 antibody; NIMA related kinase 2 antibody; NimA related protein kinase 2 antibody; NimA-like protein kinase 1 antibody; NimA-related protein kinase 2 antibody; NLK 1 antibody; NLK1 antibody; Serine/threonine-protein kinase Nek2 antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Human NEK2

Immunogen Species

Homo sapiens (Human)

Isotype

IgG

Purification Method

Antigen Affinity purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. -20°C, Avoid freeze / thaw cycles.

Tested Applications

ELISA,WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Background

Function

Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGO1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Phosphorylates CEP68 and CNTLN directly or indirectly. NEK2-mediated phosphorylation of CEP68 promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis. Involved in the regulation of centrosome disjunction.; Phosphorylates and activates NEK11 in G1/S-arrested cells.; Not present in the nucleolus and, in contrast to isoform 1, does not phosphorylate and activate NEK11 in G1/S-arrested cells.

Gene References into Functions

Taken together with the result that gamma-taxilin protein expression levels were decreased at the onset of mitosis, we propose that gamma-taxilin participates in Nek2A-mediated centrosome disjunction as a negative regulator through its interaction with Nek2A. PMID: 29225051
NEK2 can promote HCC metastasis through induction of the epithelial-mesenchymal transition. PMID: 29399700
evaluated the role of NEK2 in bone destruction of MM via clinical gene expression profiling data analysis and in vitro and in vivo mouse models. Further, treatment with the heparanase inhibitor Roneparstat, a Phase 1 drug in MM treatment,14 markedly inhibited OCL differentiation and rescued bone mass loss induced by NEK2 overexpression, suggesting that targeting NEK2 and/or its signaling pathway may be not only for preven PMID: 28400617
Nek2 is able to individually shorten cilia when cilia are growing but requires interaction with Aurora A when cilia are being absorbed. PP1 binding protein, PPP1R42, directly inhibits Nek2 and increases cilia formation. PMID: 29141582
Data suggest that low NIMA-related kinase 2 (NEK2) expression might be a useful predictor in hepatocellular carcinoma (HCC) as a poor prognostic factor, and could serve as a potential therapeutic target for HCC. PMID: 28759960
present study firstly reveals that NEK2 protein is widely overexpressed in gliomas. NEK2 overexpression correlates significantly with malignancy (WHO grades), proliferation (Ki-67) and prognosis in malignant gliomas PMID: 28321704
Results found NEK2 to be upregulated in lung cancer tissues and, its 3'-UTR mRNA is directly targeted by miR-128 to regulate its protein expression. PMID: 28514100
Displacement of Dishevelled (DVL) from the centrosome and its release into the cytoplasm on NEK2 phosphorylation is coupled to the removal of linker proteins. PMID: 27486244
NEK2 promotes aerobic glycolysis through regulating splicing of PKM and increasing the PKM2/PKM1 ratio in myeloma cells which contributes to its oncogenic activity. PMID: 28086949
Nek2A is found to stabilize SuFu at least partly depending on its kinase activity, thereby triggering phosphorylation of the SuFu protein. PMID: 27297360
NEK2 has a role in maintaining glioma stem cells in glioblastoma by stabilizing the EZH2 protein PMID: 28737508
This study reports a novel set of Dengue virus (DENV) NS1-interacting host cell proteins in the HepG2 cell line and proposes possible roles for human NEK2, TAO1, and COG1 in DENV infection. PMID: 27108190
Our findings suggested that NIMA-related kinase 2B could play a role in the development and progression of breast cancer. Combination treatment using NIMA-related kinase 2B small interfering RNA and paclitaxel might be a novel potential therapy method for breast cancer. PMID: 28475000
NEK2 modulates hepatoma cell functions, including growth, drug resistance, metastasis and angiogenesis via downstream genes activation PMID: 27925179
Alteration of NEK2 protein levels may contribute to invasion and metastasis of hepatocellular carcinoma PMID: 28101574
the functional roles of NEK2 in cancer development (review) PMID: 27019372
NEK2 was associated with unfavorable outcomes in HCC patients. PMID: 27349376
enhanced NEK2 expression in hepatocellular carcinoma (HCC) promotes HCC progression and drug resistance by promoting PP1/Akt and Wnt pathway activation, which may represent a new therapeutic target for HCC. PMID: 27509921
Our findings demonstrated that NEK2 could be a valuable carcinogenic factor and a promising therapeutic target for primary liver cancer PMID: 26337275
Suggest elevated Nek2 protein expression may be an independent risk factor for colon cancer. PMID: 26823916
Hepatocellular carcinoma patients with high NEK2 expression had significantly poor recurrence-free survival (p=0.042) and early recurrence after hepatectomy. PMID: 26851035
Cep85 is a bona fide Nek2A-binding partner that surrounds the proximal ends of centrioles where it cooperates with PP1gamma (also known as PPP1CC) to antagonize Nek2A PMID: 26220856
Kif24 is a physiological substrate of Nek2, which regulates cilia disassembly through a concerted mechanism involving Kif24-mediated microtubule depolymerization. PMID: 26290419
The results suggest that overexpression of NEK2 has the potential to serve as a biomarker for PCa prognosis. PMID: 26320076
Nek2A is degraded in mitosis regardless of enforced spindle checkpoint activation PMID: 25673878
we highlight recent findings on the aberrant expression and functional significance of NEK2A in human cancers and emphasize their significance for therapeutic potentials. PMID: 25705694
Findings are consistent with the hypothesis that NEK2 contributes to ALDH1A1-dependent drug resistance in myeloma PMID: 25230277
Nek2 was demonstrated to phosphorylate Cep68 in vivo and this phosphorylation appears to promote Cep68 degradation in mitosis. PMID: 25704143
Nek2 plays a central role in proteasome-mediated cell cycle regulation and in conferring resistance to bortezomib in cancer cells. PMID: 25313354
High expression of Nek2 could serve as an independent predictor of poor prognosis in pancreatic ductal adenocarcinoma. PMID: 24966957
Overexpression of the Nek2 kinase is associated with colorectal cancer. PMID: 25043295
Data suggest a role for Nek2 driving centrosome amplification downstream of the E2F3 activator. PMID: 24797070
These results identify a novel mechanism for regulating beta-catenin stability that is independent of GSK3beta and provide new insight into a pathway involving Plk1, Nek2, and beta-catenin that regulates the centrosome cycle. PMID: 24501426
Nek2 may represent a new potential target for non-small cell lung cancer therapeutic intervention PMID: 24763826
it appears that Nek2 and Plk4 might synergize to promote breast tumorigenesis and may also be involved in tamoxifen and trastuzumab resistance PMID: 24389189
NEK2 directly or indirectly interacts with a number of genes, proteins, microRNAs and biological processes associated with drug resistance in ovarian and other types of cancer. PMID: 24337664
Up-regulation of NEK2 by microRNA-128 methylation is associated with colorectal cancer. PMID: 24046120
Nek2 overexpression could not induce centrosome amplification and chromosome instability without expression of TRF1. PMID: 24091727
these data indicate that Nek2 has a pivotal role in breast cancer growth at primary and secondary sites, and thus may be an attractive and novel therapeutic target for this disease. PMID: 23708664
CIP2A strongly interacts with NEK2 during G2/M phase, thereby enhancing NEK2 kinase activity to facilitate centrosome separation in a PP1- and PP2A-independent manner. PMID: 24214971
NEK2 as a novel p53-repressed gene PMID: 24163369
These results suggest that the malignant transformation of plexiform neurofibroma is associated with distinct changes in the expression of BUB1B, PBK and NEK2 PMID: 23370767
Data conclude that centrosome amplification is modulated through Cdk4 and Nek2 signaling and that binucleation is a likely source of CA in Her2+ breast cancer cells. PMID: 23776583
Whole genome sequencing in patients with retinitis pigmentosa reveals pathogenic DNA structural changes and NEK2 as a new disease gene. PMID: 24043777
NEK2 phosphorylation antagonizes the microtubule stabilizing activity of centrobin. PMID: 23291182
small interfering RNA and antisense oligonucleotides against Nek2 worked synergistically with paclitaxel and doxorubicin by promoting cell apoptosis. PMID: 23340795
The recruitment of Nek2 to the proximal ends of centrioles is dependent on one of its substrates, the centrosome cohesion protein C-Nap1. PMID: 22613497
Our results indicate that genetic polymorphisms of centrobin and Nek2 are related to breast cancer susceptibility in Chinese Han women. PMID: 23001753
Nek2 C downregulation, using RNA interference, decreased the survival, invasion and migration of MCF10DCIS.com and MCF10CA1a cells as well as in human primary breast cancer tissue. PMID: 22824957
Nek2A binds with high affinity to apo-APC/C and is degraded by the pool of Cdc20 that avoids inhibition by the spindle assembly checkpoint. PMID: 23288039

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Involvement in disease

Retinitis pigmentosa 67 (RP67)

Subcellular Location

[Isoform 1]: Nucleus. Nucleus, nucleolus. Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Chromosome, centromere, kinetochore. Chromosome, centromere. Note=STK3/MST2 and SAV1 are required for its targeting to the centrosome. Colocalizes with SGO1 and MAD1L1 at the kinetochore. Not associated with kinetochore in the interphase but becomes associated with it upon the breakdown of the nuclear envelope. Has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end.; [Isoform 2]: Cytoplasm. Note=Predominantly cytoplasmic.; [Isoform 4]: Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Predominantly nuclear.

Protein Families

Protein kinase superfamily, NEK Ser/Thr protein kinase family, NIMA subfamily

Tissue Specificity

Isoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types. Isoform 1 and isoform 4 are expressed in the testis. Up-regulated in various cancer cell lines, as well as primary breast tumors.

Database Links

HGNC: 7745

OMIM: 604043

KEGG: hsa:4751

STRING: 9606.ENSP00000355966

UniGene: Hs.153704