NFKBIA Antibody

Code CSB-PA010535
Size US$167
  • Western Blot analysis of HuvEc cells using IκB-α Polyclonal Antibody
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Product Details

Uniprot No. P25963
Target Names NFKBIA
Alternative Names I kappa B alpha antibody; I-kappa-B-alpha antibody; IkappaBalpha antibody; IkB-alpha antibody; IKBA antibody; IKBA_HUMAN antibody; IKBalpha antibody; MAD 3 antibody; MAD3 antibody; Major histocompatibility complex enhancer-binding protein MAD3 antibody; NF kappa B inhibitor alpha antibody; NF-kappa-B inhibitor alpha antibody; NFKBI antibody; NFKBIA antibody; Nuclear factor of kappa light chain gene enhancer in B cells antibody; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Immunogen Synthesized peptide derived from Human IκB-α around the non-phosphorylation site of S32/S36.
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Isotype IgG
Purification Method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form Liquid
Tested Applications WB, IHC, IF, ELISA
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Protocols Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.


The novel thioredoxin reductase inhibitor A-Z2 triggers intrinsic apoptosis and shows efficacy in the treatment of acute myeloid leukemia. Dongdong Zhang, et al,Free Radical Biology and Medicine,2019

Sample type: AML cell lines
Sample species: Mouse
Application: WB
Review: AML cells were treated with 1 µM A-Z2 for 24 h, cell supernatants were harvested, cytochrome c level was measured by ELISA. Apoptosis (F) and cell cycle were analyzed after Molm-13 were treated with 1 µM A-Z2 for 24 h. AML-PC: AML patient primary cell.
PMID: 31730934

Genistein improves schistosomiasis liver granuloma and fibrosis via dampening NF-kB signaling in mice. Chunpeng al,Parasitology Research,2017

Application: Mouse liver tissues
Sample type: Peripheral blood neutrophils
Review: Representative western blots for IκB, phosphor-IκB, p65, and phospho-p65 expressions in mouse liver tissues.
PMID: 28160073

Target Data

Function Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
Gene References into Functions
  1. this study shows changes of NF-kB expression levels induced by cell-free DNA in different cell types PMID: 29743966
  2. the results of real-time PCR and western blotting revealed that Huaier extract decreased p65 and c-Met expression and increased IkappaBalpha expression, while paclitaxel increased p65 expression and reduced IkappaBalpha and c-Met expression.The molecular mechanisms may be involved in the inhibition of the NF-kappaB pathway and c-Met expression PMID: 29039556
  3. Colorectal cancer cases showed an increase in the frequency of NFkappaBIA-881G allele in Egyptian subjects. PMID: 28389768
  4. association between polymorphisms and progression of chronic hepatitis B Virus infection among Chinese Han Population PMID: 29093318
  5. miR-668 was upregulated in radioresistant human breast cancer cell lines MCF-7R and T-47DR, which targeted IkappaBalpha, activated the NF-kappaB pathway and thus increased the radioresistance of breast cancer cells. PMID: 28138801
  6. We showed that pristimerin suppressed tumor necrosis factor a (TNFalpha)-induced IkappaBa phosphorylation, translocation of p65, and expression of NFkappaB-dependent genes. Moreover, pristimerin decreased cell viability and clonogenic ability of Uveal melanoma (UM)cells. A synergistic effect was observed in the treatment of pristimerin combined with vinblastine, a frontline therapeutic agent, in UM. PMID: 28766683
  7. Data collectively demonstrate the functional importance of IkappaBalpha-mediated stripping of NFkappaB from DNA in the kinetic control of NFkappaB signaling. PMID: 28167786
  8. These findings indicate that genetic polymorphisms of NFKB1A rs696, pre-miR-146a rs2910164 and pre-miR-499 rs3746444 may represent novel markers of AT susceptibility. PMID: 28674224
  9. XPO1 inhibitor combination therapy with bortezomib or carfilzomib induces nuclear localization of IkappaBalpha and overcomes acquired proteasome inhibitor resistance in human multiple myeloma. PMID: 27806331
  10. Molecular docking analysis indicated that transcription factor NF-kappaB was one of the potential molecular targets modulated by DTTF. Specifically, the drug blocked the TNFalpha-induced phosphorylation of upstream IkappaBalpha kinase in a time-dependent manner leading to the suppression of NF-kappaB activation and nuclear translocation PMID: 27882436
  11. We show that HOTAIR regulates activation of NF-kB by decreasing Ik-Ba (NF-kB inhibitor) and establish that by inducing prolonged NF-kB activation and expression of NF-kB target genes during DNA damage, HOTAIR has a critical role in cellular senescence and platinum sensitivity PMID: 27041570
  12. Here, the authors report amide hydrogen/deuterium exchange data that reveal long-range allosteric changes in the NFkappaB (RelA-p50) heterodimer induced by DNA or IkappaBalpha binding. PMID: 28249778
  13. Sam68 is essential for DNA damage-induced NF-kappaB activation and colon tumorigenesis. PMID: 27458801
  14. Specifically, BCA2 serves as an E3 SUMO ligase in the SUMOylation of IkappaBalpha, which in turn enhances the sequestration of NF-kappaB components in the cytoplasm. Since HIV-1 utilizes NF-kappaB to promote proviral transcription, the BCA2-mediated inhibition of NF-kappaB significantly decreases the transcriptional activity of HIV-1. PMID: 28122985
  15. This study found that NFKBIA mRNAs was significantly expressed in normal tissues compared with glioma specimens. PMID: 27538656
  16. Findings indicate the prognostic value of NFKB inhibitor alpha (NFKBIA) in lower-grade gliomas (LGGs). PMID: 27052952
  17. W346 effectively inhibited tumor necrosis factor (TNF-a)-induced NF-kappaB activation by suppressing IKK phosphorylation, inhibiting IkB-a degradation, and restraining the accumulation of NF-kappaB subunit p65 nuclear translocation. W346 also affected NF-kappaB-regulated downstream products involved in cycle arrest and apoptosis. PMID: 26520440
  18. treating cells with SZC014 resulted in a decrease in phosphorylation of IkBa and NF-kappaB/p65 and NF-kappaB/p65 nuclear translocation. The cytotoxic activities of seven OA derivatives were generally stronger than that of OA, among which, SZC014 possessed the most potent anticancer activity in SGC7901 cells and would be a promising chemotherapic agent for the treatment of gastric cancer PMID: 26547583
  19. Network analysis identified NFKBIA as pathogenic gene in childhood asthma. PMID: 27420950
  20. HMBA was able to increase prostratin-induced phosphorylation and degradation of NF-kappaB inhibitor IkappaBalpha, thereby enhancing and prolonging prostratin-induced nuclear translocation of NF-kappaB, a prerequisite for stimulation of transcription initiation PMID: 27529070
  21. enhanced miR-381a-3p expression contributed to the injury of osteoarthritis mainly by inhibiting the expression of IkappaBalpha. PMID: 27312547
  22. Timely and efficient degradation of ubiquitinated IkappaB[alpha], concomitant with timely and efficient liberation of RelA from ubiquitinated IkappaB[alpha] and RelA nuclear translocation, essentially depends on the presence of functional p97/VCP. PMID: 26463447
  23. Activated Rac1 regulates the degradation of IkappaBalpha and the nuclear translocation of STAT3-NFkappaB complexes in starved cancer cells PMID: 27151455
  24. mutation in Chinese patient results in mycobacterial infections without ectodermal dysplasia PMID: 26691317
  25. DAT stabilized IkBa by inhibiting the phosphorylation of Ika by the IkB kinase (IKK) complex. DAT induced proteasomal degradation of TRAF6, and DAT suppressed IKKb-phosphorylation through downregulation of TRAF6 PMID: 26647777
  26. the rs3138053 polymorphism of NFKBIA gene is a candidate for susceptibility to overall cancers, while rs696 plays a protective role [meta-analysis] PMID: 26488500
  27. Identify a novel BCR-ABL/IkappaBalpha/p53 network, whereby BCR-ABL functionally inactivates a key tumor suppressor in chronic myeloid leukemia. PMID: 26295305
  28. Study demonstrates an association between functional polymorphisms of IkappaBalpha rs696 and smoking with the risk of defective spermatogenesis, suggesting some interaction between the NF-kappaB signalling pathway and smoking-related ROS in human spermatogenesis. PMID: 25352423
  29. genetic variation associated with susceptibility to acute kidney injury PMID: 26477820
  30. MicroRNA-19a mediates gastric carcinoma cell proliferation through the activation of IkappaBalpha. PMID: 26239140
  31. No association was observed between NFKBIA variants and risk of liver cancer. PMID: 24578542
  32. SM22alpha is a phosphorylation-regulated suppressor of IKK-IkappaBalpha-NF-kappaB signaling cascades. PMID: 25937534
  33. Data suggest that activity of IKBalpha can be regulated by dietary factors; dietary supplementation with luteolin inhibits vascular endothelial inflammation by suppressing IKBalpha/NFkappaB signaling. PMID: 25577468
  34. This review and meta-analysis of the association of NFKBIA -881 A>G polymorphism with cancer susceptibility reveals that -881 A>G polymorphism may increase risk of cancer development in Asian populations. PMID: 26252270
  35. miR-126 may play an important role in hepatic fibrosis by downregulating the expression of IkappaBalpha partly through the NF-kappaB signaling pathway. PMID: 25974152
  36. IkappaBetaalpha inhibits apoptosis at the outer mitochondrial membrane independently of NF-kappaB retention. PMID: 25361605
  37. The single nucleotide polymorphism rs1957106 CT and TT genotypes were found to be associated with lower NFKBIA protein levels and a poor prognosis of pateints with glioblastoma. PMID: 25215581
  38. data suggest that the NFKBIA 126 G/A polymorphism might be potentially helpful to identify liver transplant recipients with an increased susceptibility to develop recurrent acute rejections PMID: 25112903
  39. Expression of IkappaBalpha in human bladder cancer cells is negatively correlated with epithelial-mesenchymal transition and tumor invasion in vitro. PMID: 25374080
  40. NFKBIA-rs2233419AG was associated with a significantly increased risk of developing recurrent wheezing. PMID: 25326706
  41. miR-196a can directly interact with IkappaBalpha 3'-UTR to suppress IkappaBalpha expression and subsequently promote activation of NF-kappaB PMID: 24463357
  42. MiR-196a promotes pancreatic cancer progression by targeting nuclear factor kappa-B-inhibitor alpha. PMID: 24504166
  43. Data indicate that following bortezomib treatment there was accumulation of IkappaB-alpha (IkappaBalpha) without affecting its phosphorylation status at an early time point. PMID: 23697845
  44. This study reveals that polymorphisms in the IkB-alpha promoter (-881 A/G, -826 C/T) are strongly associated with the susceptibility of Iranian Multiple Sclerosis patients PMID: 24368589
  45. The results of this study suggested that oligodendroglial IkappaBalpha expression and NF-kappaB are activated early in the course of MSA and their balance contributes to the decision of cellular demise. PMID: 24361600
  46. No statistically significant CRC risk association was found for the NFKBIA polymorphisms. PMID: 23996241
  47. data indicate that NFKBIA deletions are present but not frequent in Glioblastoma multiforme (GBM); the deletions become frequent in GBM neurospheres (NS), an event that seems to be affected by the presence of EGF in the culture medium PMID: 24330732
  48. the analysis of IkappaBalpha expression at salivary gland epithelial cell level could be a potential new hallmark of Sjogren's syndrome progression. PMID: 23377923
  49. IkappaBalpha sequesters not only p65 but also RPS3 in the cytoplasm. PMID: 24457201
  50. NF-kB expression was downregulated and its cytoplasmatic inhibitor IKBalpha was increased in CTLA4-Ig treated macrophages. PMID: 24295474
  51. Data indicate that the p97-UFD1L-NPL4 protein complex specifically associates with ubiquitinated IkappaBalpha via the interactions between p97 and the SCF(beta-TRCP) ubiquitin ligase. PMID: 24248593
  52. long-term incubation with PIs activates NF-kappaB, which is mediated by IkappaBalpha degradation via the lysosome in an IKK-dependent and IKK-independent manner. PMID: 24085292
  53. Epithelial-mesenchymal transformation, along with acquisition of a tumor stem cell-like phenotype mediated by IKKbeta/IkappaBalpha/RelA signal pathway via Snail, contributes to a low concentration of arsenite-induced tumorigenesis. PMID: 23069812
  54. These data show that I-kappa-B-alpha can be used as a prognostic marker and target for therapy in Activated B-cell lymphoma, one of the three subtypes of Diffuse Large B-cell Lymphoma. PMID: 23555990
  55. The findings from this human cell study in vitro indicate that both relatively high single-dose and chronic opioid exposure may induce the structural changes in the developing human brain and the adult brain by altering the expression of neuronal differentiation- and neurite outgrowth-related genes IkappaBa and TrkB in vivo. PMID: 23422506
  56. the silencing of NFKBIA may play an important role in the carcinogenesis of adenocarcinomas independent of EGFR/K-ras mutations or EML4-ALK fusion. PMID: 23439505
  57. NFKB1 -94 Ins promoter polymorphism increased the risk of hepatocellular carcinoma (HCC), and may be applied as a predictive factor for the clinical stage and tumor size in female HCC patients. PMID: 23457512
  58. miR-126 may play roles in UC inflammatory activity by down-regulating the expression of IKBA, an important inhibitor of NF-kappaB signaling pathway. PMID: 23285182
  59. Immunologically relevant genetic variation in the promoter of NFKBIA is differentially susceptibile to severe bronchiolitis following infection with respiratory syncytial virus, airway hyperresponsiveness, and severe bronchopulmonary dysplasia. PMID: 23487427
  60. Pokemon activates the expression of both p65 and IkappaBalpha by sequence-specific binding to their promoters and plays a dual role in regulating NF-kappaB signaling. PMID: 23054188
  61. Authors discover that HIV-1 infection elevates the phosphorylation of IkappaBalpha and p100, and that this increase is greatly reduced when a Vpr-negative HIV-1 is used for infection. PMID: 23453579
  62. Our findings clearly demonstrate changes in the levels of IkappaBalpha in Sjogren's syndrome monocytes, suggesting that the attenuated expression of IkappaBalpha could contribute to the deregulation of NF-kappaB pathways in Sjogren's syndrome pathogenesis. PMID: 22935973
  63. YLTA mutations stabilize the I-kappa-B-alpha to proteasomal degradation. PMID: 23274114
  64. TOPK directly interacted with and phosphorylated IkappaBalpha at Ser-32, leading to p65 nuclear translocation and NF-kappaB activation. PMID: 23250755
  65. The expressions of NF-kappaBp65 and IkappaBalpha are negatively correlated in gestational trophoblastic tumor tissues. PMID: 23046941
  66. bortezomib-induced autophagy confers relative DLBCL cell drug resistance by eliminating I-kappaBalpha. PMID: 22393418
  67. MIB1 negatively regulates TNFalpha- and IL1beta- induced NF-kappaB target gene activation PMID: 22184009
  68. The endothelium plays important roles in obesity- and age-related disorders through intracellular NF-kappaB signaling, thereby ultimately affecting life span. PMID: 22302838
  69. A novel mutation in the IKBA gene was discovered in an infant with autosomal-dominant anhidroticectodermal dysplasia with immune deficiency syndrome PMID: 22078572
  70. in glioma cell lines that microRNA-30e* (miR-30e*) directly targets the IkappaBalpha 3'-UTR and suppresses IkappaBalpha expression PMID: 22156201
  71. These results suggest the existence of a reservoir of monoubiquitylated IkappaBalpha resistant to TNFalpha-induced proteolysis. PMID: 22022389
  72. The allele frequency and genotype distribution of NFKBIA gene polymorphism did not differ between myocardial infarction and control group. PMID: 20304615
  73. deletion of NFKBIA and amplification of EGFR show a pattern of mutual exclusivity in glioblastoma multiforme[review] PMID: 21778941
  74. Data show that IkappaB kinase beta (IKKbeta) can be activated in the nucleus by Nkx3.2 in the absence of exogenous IKK-activating signals, allowing constitutive nuclear degradation of IkappaB-alpha. PMID: 21606193
  75. CCL5 signaling induces GLI2 through a PI3K-AKT-IkappaBalpha-p65 pathway and requires GLI2 transcriptional activity to modulate IL-6 expression and Ig secretion in vitro and in vivo PMID: 21454528
  76. NFKBIA low mutation frequency plays a minor role in the NF-kappaB activity of nodular lymphocyte-predominant Hodgkin's lymphoma (NLPHL), suggesting different mechanisms of NF-kappaB activation in NLPHL compared to classical Hodgkin's lymphoma (cHL). PMID: 19648161
  77. IkBalpha promoter polymorphisms are related to susceptibility to ankylosing spondylitis in Taiwan. PMID: 19363678
  78. rs17103265 deletion homozygote is a genetic risk factor for gastric cancer, especially for certain subtypes in a southern Chinese population PMID: 21376060
  79. alpha-1-antitrypsin blocks nuclear translocation of NF-kappaB p50/p65 despite an unexpected elevation in associated phosphorylated and ubiquitinated IkappaBalpha. PMID: 21263074
  80. gene polymorphisms is associated with the development of asthma in Korea PMID: 20674643
  81. This meta-analysis demonstrates that autoimmune and inflammatory diseases are associated with NFKBIA gene -826C/T polymorphism, but not with 2758A/G, -881A/G, and -279C/T. PMID: 20495844
  82. promoter polymorphism is associated with susceptibility to rheumatoid arthritis in Taiwain PMID: 20563630
  83. Deletion of NFKBIA has an effect that is similar to the effect of EGFR amplification in the pathogenesis of glioblastoma and is associated with comparatively short survival. PMID: 21175304
  84. Polymorphism in NFkB was associated with colorectal cancer. PMID: 20836841
  85. alpha-mangostin also strongly inhibited PMA-induced degradation of inhibitor of kappaBalpha (IkappaBalpha) PMID: 20652762
  86. Although p65 recruitment to TNF-alpha, IL-1ss, and IL-6 promoters is inhibited by nuclear IkappaBkappaalpha, recruitment to interleukin (IL)-8 promoter is not repressed. PMID: 20696864
  87. Total and phosphorylated IkappaBalpha protein expression might serve as markers for NF-kappaB activation in human ovarian carcinoma. PMID: 20546338
  88. Nuclear IKKbeta acts as an adaptor protein for IkappaBalpha degradation in UV-induced NF-kappaB activation. NF-kappaB activated by the nuclear IKKbeta adaptor protein suppresses anti-apoptotic gene expression and promotes UV-induced cell death. PMID: 20797629
  89. Data show that RSK2 is activated by treatment with tumor necrosis factor-alpha (TNF-alpha) and directly phosphorylates IkappaBalpha at Ser-32, leading to IkappaBalpha degradation. PMID: 20385620
  90. TGase 2-mu-calpain system is significant in the NF-kappaB pathway via I-kappaBalpha polymerization and subsequent degradation. PMID: 20659425
  91. Subjects with at least one A allele for NFKBIA rs1951276 had approximately 29% lower insulin sensitivity compared to individuals homozygous for the G allele... PMID: 19798070
  92. Proteasome inhibitor PS-341 (bortezomib) induces calpain-dependent IkappaB(alpha) degradation. PMID: 20335171
  93. I kappaB alpha rs2233408 T heterozygotes were associated with reduced risk of gastric cancer, especially for the development of certain subtypes of gastric cancer in Chinese population. PMID: 20132559
  94. IKBA -826T allele, IKBA -881A -826T -550A -519C -297C haplotype, and IKBA -881A -826T -550A -519T -297C haplotype may be related to susceptibility to Behcet's disease. IKBA -826T/T is associated with skin lesions in patients with Behcet's disease. PMID: 20164548
  95. NFKBIA mRNA was strongly expressed in H-RS cells from Hodgkin lymphoma sections, and little was detected in the reactive surrounding lymphocytes. PMID: 20193848
  96. Case report. this patient's IkappaBalpha mutation caused GH and IGF-l resistance which, in turn, contributed to his growth failure. PMID: 20080849
  97. Aurora-A, via activating Akt, stimulated nuclear factor-kappaB signaling pathway to promote cancer cell survival. PMID: 19891769
  98. IkappaBalpha x p53 complex plays an important role in responses involving growth regulation, apoptosis, and hypoxic stress PMID: 11799106
  99. A novel in vitro assay for deubiquitination of I kappa B alpha PMID: 11913973
  100. NF-kappa Beta activation in BAL cells may play in important role in initiation and progression of silica-induced pulmonary inflammation, cellular damage, and fibrosis PMID: 12028809

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Involvement in disease Ectodermal dysplasia, anhidrotic, with T-cell immunodeficiency autosomal dominant (ADEDAID)
Subcellular Location Cytoplasm, Nucleus
Protein Families NF-kappa-B inhibitor family
Database Links

HGNC: 7797

OMIM: 164008

KEGG: hsa:4792

STRING: 9606.ENSP00000216797

UniGene: Hs.81328

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