NSMAF Antibody

Datasheet

Code	CSB-PA856416LA01HU
Size	US$166
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Image	IHC image of CSB-PA856416LA01HU diluted at 1:300 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of A549 cells with CSB-PA856416LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) NSMAF Polyclonal antibody

Uniprot No.

Q92636

Target Names

NSMAF

Alternative Names

NSMAF antibody; FANProtein FAN antibody; Factor associated with neutral sphingomyelinase activation antibody; Factor associated with N-SMase activation antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Protein FAN protein (1-233AA )

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The NSMAF Antibody (Product code: CSB-PA856416LA01HU) is Non-conjugated. For NSMAF Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA856416LB01HU	NSMAF Antibody, HRP conjugated	ELISA
FITC	CSB-PA856416LC01HU	NSMAF Antibody, FITC conjugated
Biotin	CSB-PA856416LD01HU	NSMAF Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Couples the p55 TNF-receptor (TNF-R55 / TNFR1) to neutral sphingomyelinase (N-SMASE). Specifically binds to the N-smase activation domain of TNF-R55. May regulate ceramide production by N-SMASE.

Gene References into Functions

these data identify a novel caspase-2-interacting factor, FAN, and expand the role for the enzyme in seemingly non-apoptotic cellular mechanisms. PMID: 29621545
The rs1050504 C > T genotype was observed to be significantly associated with an increased risk for developing pulmonary tuberculosis. PMID: 29115933
The interaction of FAN with receptor for activated C-kinase 1 (RACK1) appears to be dependent on the folding of the WD repeats into a secondary structure, because no linear binding motifs are identified in the WD-repeat region of FAN. PMID: 12391233
sphingolipid activator proteins like domain may stabilize the fold of acid sphingomyelinase PMID: 15653433
Regulation of NSMAF by TNF-alpha involves PRKCD in lung epithelial cells. PMID: 18653803

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Tissue Specificity

Ubiquitous.

Database Links

HGNC: 8017

OMIM: 603043

KEGG: hsa:8439

STRING: 9606.ENSP00000411012

UniGene: Hs.372000

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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