NUBP2 Antibody

Datasheet

Code	CSB-PA896918LA01HU
Size	US$166
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Image	IHC image of CSB-PA896918LA01HU diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA896918LA01HU at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) NUBP2 Polyclonal antibody

Uniprot No.

Q9Y5Y2

Target Names

NUBP2

Alternative Names

C447E6.1 (nucleotide binding protein 1 (E.coli MinD like) ) antibody; CFD1 antibody; Cytosolic Fe-S cluster assembly factor NUBP2 antibody; D17Wsu11e antibody; Homolog of yeast cytosolic FeS cluster deficient 1 antibody; NBP 2 antibody; NUBP1 antibody; Nubp2 antibody; NUBP2_HUMAN antibody; Nucleotide binding protein 2 (E.coli MinD like) antibody; Nucleotide binding protein 2 (MinD homolog, E. coli) antibody; Nucleotide binding protein 2 antibody; Nucleotide-binding protein 2 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Cytosolic Fe-S cluster assembly factor NUBP2 protein (54-170AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The NUBP2 Antibody (Product code: CSB-PA896918LA01HU) is Non-conjugated. For NUBP2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA896918LB01HU	NUBP2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA896918LC01HU	NUBP2 Antibody, FITC conjugated
Biotin	CSB-PA896918LD01HU	NUBP2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:20-1:200
IF	1:20-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function

Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Negatively regulates cilium formation and structure.

Subcellular Location

Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm. Cytoplasm, cytoskeleton, cilium axoneme. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cytoplasm, cytoskeleton, microtubule organizing center.

Protein Families

Mrp/NBP35 ATP-binding proteins family, NUBP2/CFD1 subfamily

Tissue Specificity

Widely expressed with highest expression in skeletal muscle.

Database Links

HGNC: 8042

OMIM: 610779

KEGG: hsa:10101

STRING: 9606.ENSP00000262302

UniGene: Hs.256549

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Phage Display Service

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Protein Service

Gene Synthesis Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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