NUDT5 Antibody

Datasheet

Code	CSB-PA891535LA01HU
Size	US$166
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Image	IHC image of CSB-PA891535LA01HU diluted at 1:600 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA891535LA01HU diluted at 1:600 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA891535LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) NUDT5 Polyclonal antibody

Uniprot No.

Q9UKK9

Target Names

NUDT5

Alternative Names

ADP sugar pyrophosphatase antibody; ADP-sugar pyrophosphatase antibody; hYSAH 1 antibody; hYSAH1 antibody; Nucleoside diphosphate linked moiety X motif 5 antibody; Nucleoside diphosphate linked moiety X type motif 5 antibody; Nucleoside diphosphate-linked moiety X motif 5 antibody; Nudix (nucleoside diphosphate linked moiety X) type motif 5 antibody; Nudix motif 5 antibody; Nudix type motif 5 antibody; NUDT 5 antibody; Nudt5 antibody; NUDT5_HUMAN antibody; YSA1 antibody; YSA1H antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human ADP-sugar pyrophosphatase protein (34-166AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The NUDT5 Antibody (Product code: CSB-PA891535LA01HU) is Non-conjugated. For NUDT5 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA891535LB01HU	NUDT5 Antibody, HRP conjugated	ELISA
FITC	CSB-PA891535LC01HU	NUDT5 Antibody, FITC conjugated
Biotin	CSB-PA891535LD01HU	NUDT5 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:500-1:1000
IF	1:200-1:500

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Enzyme that can either act as an ADP-sugar pyrophosphatase in absence of diphosphate or catalyze the synthesis of ATP in presence of diphosphate. In absence of diphosphate, hydrolyzes with similar activities various modified nucleoside diphosphates such as ADP-ribose, ADP-mannose, ADP-glucose, 8-oxo-GDP and 8-oxo-dGDP. Can also hydrolyze other nucleotide sugars with low activity. In presence of diphosphate, mediates the synthesis of ATP in the nucleus by catalyzing the conversion of ADP-ribose to ATP and ribose 5-phosphate. Nuclear ATP synthesis takes place when dephosphorylated at Thr-45. Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming. Does not play a role in U8 snoRNA decapping activity. Binds U8 snoRNA.

Gene References into Functions

Data suggest that targeting nudix hydrolase 5 (NUDT5) may represent a promising new therapeutic approach for breast cancer treatment. PMID: 29343827
In the presence of pyrophosphate, ADP-ribose is used by the pyrophosphatase NUDIX5 to generate nuclear ATP. PMID: 27257257
The NUDT5 protein could play significant roles in the prevention of RNA oxidation and survival in human fibroblast cells. PMID: 23581889
Results suggest that the NUDT5 protein may play significant roles in regulating the G1-S transition in HeLa cells. PMID: 22200976
The human NUDT5, which has an intrinsic activity to cleave ADP sugars to AMP and sugar phosphate, possesses the ability to degrade 8-oxo-dGDP to the monophosphate. PMID: 21389046
The broad substrate specificity of hNUDT5 is achieved by a diversity of not only substrate recognition, but also hydrolysis mechanisms. PMID: 21768126
human MTH1, MTH2, and NUDT5 proteins act as a defense against the mutagenesis induced by oxidized dGTP. PMID: 20144704
NUDT5 protein eliminates various oxidized deoxyribonucleoside diphosphates from the nucleotide pool and prevents their toxic effects. PMID: 19699693
degrades 8-oxo-dGDP to 8-oxo-dGMP, an unusable form for DNA synthesis, and promotes the cleavage of 8-oxo-dGTP by MTH1 to yield 8-oxo-dGMP also PMID: 12717453
Results report the crystal structure of hNUDT5 in complex with a non-hydrolyzable ADPR analogue, alpha,beta-methyleneadenosine diphosphoribose, and three Mg(2+) ions representing the transition state of the enzyme during catalysis. PMID: 18462755

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Subcellular Location

Nucleus.

Protein Families

Nudix hydrolase family

Tissue Specificity

Widely expressed. Most abundant in liver.

Database Links

HGNC: 8052

OMIM: 609230

KEGG: hsa:11164

STRING: 9606.ENSP00000419628

UniGene: Hs.555956

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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