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| Code | CSB-RA936419A0HU |
| Size | US$210 |
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| Application | Recommended Dilution |
|---|---|
| WB | 1:500-1:2000 |
| FC | 1:50-1:200 |
The creation of the NUDT5 recombinant monoclonal antibody follows a systematic procedure to ensure its quality and specificity. Initially, B cells are isolated from an immunized animal using a synthesized peptide derived from human NUDT5 as the immunogen. Subsequently, total RNA is extracted from the harvested B cells and converted into cDNA through reverse transcription. The NUDT5 antibody genes are then amplified using PCR with primers specific to the antibody constant regions and cloned into an expression vector. This vector is transfected into host cells to enable antibody production. The resulting NUDT5 recombinant monoclonal antibodies are collected from the cell culture supernatant and purified using affinity chromatography. Extensive characterization, including ELISA, WB, and FC analysis, is conducted to validate the antibody's specificity and its ability to recognize human NUDT5 protein.
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