NUP85 Antibody

Datasheet

Code	CSB-PA874821LA01HU
Size	US$166
	Order now
Image	Western Blot Positive WB detected in: HEK293 whole cell lysate, NIH/3T3 whole cell lysate, Mouse kidney tissue All lanes: NUP85 antibody at 2.7μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 76, 53, 70 kDa Observed band size: 76, 70 kDa Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA874821LA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human pancreatic tissue using CSB-PA874821LA01HU at dilution of 1:100 Immunofluorescent analysis of MCF-7 cells using CSB-PA874821LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Promotion
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) NUP85 Polyclonal antibody

Uniprot No.

Q9BW27

Target Names

NUP85

Alternative Names

85 kDa nucleoporin antibody; FLJ12549 antibody; FROUNT antibody; Frount protein antibody; Nuclear pore complex protein nucleoporin 85 kDa antibody; nuclear pore complex protein nucleoporin Nup75 antibody; Nuclear pore complex protein Nup85 antibody; Nucleoporin 85 antibody; Nucleoporin 85kDa antibody; Nucleoporin nucleoporin 85kDa antibody; Nucleoporin Nup75 antibody; Nucleoporin NUP85 antibody; nucleoporin, 75-KD antibody; Nucleoporin85 antibody; Nup 75 antibody; NUP 85 antibody; Nup75 antibody; nup85 antibody; NUP85_HUMAN antibody; PCN antibody; PCNT 1 antibody; PCNT antibody; PCNT, formerly antibody; PCNT1 antibody; Pericentrin antibody; Pericentrin, formerly antibody; Pericentrin-1 antibody; Pericentrin1 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Nuclear pore complex protein Nup85 protein (201-500AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The NUP85 Antibody (Product code: CSB-PA874821LA01HU) is Non-conjugated. For NUP85 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA874821LB01HU	NUP85 Antibody, HRP conjugated	ELISA
FITC	CSB-PA874821LC01HU	NUP85 Antibody, FITC conjugated
Biotin	CSB-PA874821LD01HU	NUP85 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:2000-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Essential component of the nuclear pore complex (NPC) that seems to be required for NPC assembly and maintenance. As part of the NPC Nup107-160 subcomplex plays a role in RNA export and in tethering NUP96/Nup98 and NUP153 to the nucleus. The Nup107-160 complex seems to be required for spindle assembly during mitosis. NUP85 is required for membrane clustering of CCL2-activated CCR2. Seems to be involved in CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to the phosphatidyl-inositol 3-kinase-Rac-lammellipodium protrusion cascade. Involved in nephrogenesis.

Gene References into Functions

results support a model of CCR2 equilibrium: chemokine binding changes the conformational equilibrium of CCR2 toward the active state, and Pro-C switches its binding partner from the membrane to FROUNT PMID: 25283965
Residues on the hydrophobic side are completely conserved among FROUNT-binding receptors. PMID: 24128342
Data show that the C Terminus of Nup120 Directly Binds Nup145C and Nup85. PMID: 19576787
The CCL2/CCR2 pathway via FROUNT may influence the clinical severity of CHF. PMID: 17395051
FROUNT binds to the C-terminal domain of CCR5 in addition to that of CCR2. PMID: 19841162

Hide All

Subcellular Location

Nucleus, nuclear pore complex. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Cytoplasm. Nucleus membrane.

Protein Families

Nucleoporin Nup85 family

Database Links

HGNC: 8734

OMIM: 170285

KEGG: hsa:79902

STRING: 9606.ENSP00000245544

UniGene: Hs.362817

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
If CAPTCHA verification fails, click to refresh the CAPTCHA and try again.

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address