PAFAH1B3 Antibody

Datasheet

Code	CSB-PA619963LA01HU
Size	US$166
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Image	IHC image of CSB-PA619963LA01HU diluted at 1:600 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA619963LA01HU diluted at 1:600 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of A549 cells with CSB-PA619963LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PAFAH1B3 Polyclonal antibody

Uniprot No.

Q15102

Target Names

PAFAH1B3

Alternative Names

PA1B3_HUMAN antibody; PAF acetylhydrolase 29 kDa subunit antibody; PAF AH 29 kDa subunit antibody; PAF AH subunit gamma antibody; PAF-AH 29 kDa subunit antibody; PAF-AH subunit gamma antibody; PAFAH subunit gamma antibody; Pafah1b3 antibody; PAFAHG antibody; Platelet activating factor acetylhydrolase IB subunit gamma antibody; Platelet activating factor acetylhydrolase isoform Ib gamma subunit (29kD) antibody; Platelet activating factor acetylhydrolase isoform Ib gamma subunit 29kDa antibody; Platelet activating factor acetylhydrolase isoform Ib gamma subunit antibody; Platelet-activating factor acetylhydrolase IB subunit gamma antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Platelet-activating factor acetylhydrolase IB subunit gamma protein (103-187AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The PAFAH1B3 Antibody (Product code: CSB-PA619963LA01HU) is Non-conjugated. For PAFAH1B3 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA619963LB01HU	PAFAH1B3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA619963LC01HU	PAFAH1B3 Antibody, FITC conjugated
Biotin	CSB-PA619963LD01HU	PAFAH1B3 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:500-1:1000
IF	1:200-1:500

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Alpha1 catalytic subunit of the cytosolic type I platelet-activating factor (PAF) acetylhydrolase (PAF-AH (I)) heterotetrameric enzyme that catalyzes the hydrolyze of the acetyl group at the sn-2 position of PAF and its analogs and modulates the action of PAF. The activity and substrate specificity of PAF-AH (I) are affected by its subunit composition. Both alpha1/alpha1 homodimer (PAFAH1B3/PAFAH1B3 homodimer) and alpha1/alpha2 heterodimer(PAFAH1B3/PAFAH1B2 heterodimer) hydrolyze 1-O-alkyl-2-acetyl-sn-glycero-3-phosphoric acid (AAGPA) more efficiently than PAF, but they have little hydrolytic activity towards 1-O-alkyl-2-acetyl-sn-glycero-3-phosphorylethanolamine (AAGPE). Plays an important role during the development of brain.

Gene References into Functions

PAFAH1B3 as a critical metabolic node in breast cancer PMID: 24954006
PAFAH1B3 has been found to be differentially methylated in head and neck squamous cell carcinoma. PMID: 22461910

Subcellular Location

Cytoplasm.

Protein Families

'GDSL' lipolytic enzyme family, Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily

Tissue Specificity

In the adult, expressed in brain, skeletal muscle, kidney, thymus, spleen, colon, testis, ovary and peripheral blood leukocytes. In the fetus, highest expression occurs in brain.

Database Links

HGNC: 8576

OMIM: 603074

KEGG: hsa:5050

STRING: 9606.ENSP00000262890

UniGene: Hs.466831

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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