PARP3 Antibody

Code CSB-PA017467EA01HU
Size US$166
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Image
  • Western Blot
    Positive WB detected in: A549 whole cell lysate, LO2 whole cell lysate
    All lanes: PARP3 antibody at 3.5µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 61 kDa
    Observed band size: 61 kDa

  • IHC image of CSB-PA017467EA01HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA017467EA01HU diluted at 1:100 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) PARP3 Polyclonal antibody
Uniprot No.
Target Names
PARP3
Alternative Names
ADP ribosyltransferase (NAD+, poly (ADP ribose) antibody; ADP ribosyltransferase diphtheria toxin like 3 antibody; ADPRT-3 antibody; ADPRT3 antibody; ADPRTL2 antibody; ADPRTL3 antibody; ARTD3 antibody; hPARP 3 antibody; hPARP-3 antibody; hPARP3 antibody; IRT 1 antibody; IRT1 antibody; NAD(+) ADP ribosyltransferase 3 antibody; NAD(+) ADP-ribosyltransferase 3 antibody; NAD+ ADP ribosyltransferase 3 antibody; pADPRT 3 antibody; pADPRT-3 antibody; pADPRT3 antibody; PARP 3 antibody; PARP-3 antibody; PARP3 antibody; PARP3_HUMAN antibody; Poly (ADP ribose) polymerase family, member 3 antibody; Poly (ADP ribose) synthetase 3 antibody; Poly [ADP-ribose] polymerase 3 antibody; Poly[ADP ribose] synthetase 3 antibody; Poly[ADP-ribose] synthase 3 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Poly [ADP-ribose] polymerase 3 protein (1-240AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The PARP3 Antibody (Product code: CSB-PA017467EA01HU) is Non-conjugated. For PARP3 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA017467EB01HU PARP3 Antibody, HRP conjugated ELISA
FITC CSB-PA017467EC01HU PARP3 Antibody, FITC conjugated
Biotin CSB-PA017467ED01HU PARP3 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins and plays a key role in the response to DNA damage. Mediates mono-ADP-ribosylation of glutamate, aspartate or lysine residues on target proteins. In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation. Associates with a number of DNA repair factors and is involved in the response to exogenous and endogenous DNA strand breaks. Together with APLF, promotes the retention of the LIG4-XRCC4 complex on chromatin and accelerate DNA ligation during non-homologous end-joining (NHEJ). Cooperates with the XRCC5-XRCC6 (Ku80-Ku70) heterodimer to limit end-resection thereby promoting accurate NHEJ. Involved in DNA repair by mediating mono-ADP-ribosylation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism, such as XRCC5 and XRCC6. ADP-ribosylation follows DNA damage and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. May link the DNA damage surveillance network to the mitotic fidelity checkpoint. In addition to proteins, also able to ADP-ribosylate DNA: mediates DNA mono-ADP-ribosylation of DNA strand break termini via covalent addition of a single ADP-ribose moiety to a 5'- or 3'-terminal phosphate residues in DNA containing multiple strand breaks. Acts as a negative regulator of immunoglobulin class switch recombination, probably by controlling the level of AICDA /AID on the chromatin.
Gene References into Functions
  1. These data identify PARP3 as a molecular sensor of nicked nucleosomes. PMID: 27530147
  2. PARP3 controls of TGFbeta-induced epithelial mesenchymal transformation and acquisition of stem-like cell features by stimulation transglutaminase 2/SNAI1 signaling. PMID: 27579892
  3. Data indicate that PARP3, a DNA damage-activated ADP-ribosyltransferase, can mono-ADP-ribosylate double-stranded DNA ends. PMID: 29054115
  4. In a process of single-strand DNA repair, PARP3 mono-ADP-ribosylates nucleosomal histone H2B. PMID: 27716488
  5. we found that PARP3 interacted with FoxM1 to enhance its transcriptional activity and conferred glioblastoma cell radioresistance. Thus, our data suggest that PARP3 could be a therapeutic target to overcome radioresistance in glioblastoma PMID: 26040766
  6. Identification of ADP-ribosylation sites in PARP3 and the determination of the extent ofpoly(ADP-ribosyl)ated residues in this protein was performed. PMID: 25800440
  7. MiR-630 reduced apoptosis by downregulating several apoptotic modulators, PARP3, DDIT4, and EP300. PMID: 25255219
  8. In some cancer cells, repression of PARP3 could be responsible for an increased telomerase activity. PMID: 24528514
  9. PARP3 likely facilitates the recruitment of Ku80 to double strand breaks to antagonize DNA end resection but facilitate Ku-mediated accurate classical non-homologous end-joining. PMID: 24598253
  10. PARP3 gene occupancy in the human neuroblastoma cell line SK-N-SH occurs preferentially with developmental genes regulating cell fate specification, tissue patterning, craniofacial development and neurogenesis. PMID: 21264220
  11. PARP3 is a critical player in the stabilization of the mitotic spindle and in telomere integrity by associating and regulating the mitotic components NuMA and tankyrase 1. PMID: 21270334
  12. PARP-3 has a role in chromosomal DNA double-strand break repair. PMID: 21211721
  13. the interaction between PARP-1 and PARP-3 is unrelated to DNA single-strand break repair PMID: 20064938
  14. PARP-3 is a nuclear protein involved in transcriptional silencing and in the cellular response to DNA damage PMID: 16924674

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Subcellular Location
Nucleus. Chromosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole.
Tissue Specificity
Widely expressed; the highest levels are in the kidney, skeletal muscle, liver, heart and spleen; also detected in pancreas, lung, placenta, brain, leukocytes, colon, small intestine, ovary, testis, prostate and thymus.
Database Links

HGNC: 273

OMIM: 607726

KEGG: hsa:10039

STRING: 9606.ENSP00000381740

UniGene: Hs.271742

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