PHF10 Antibody

Datasheet

Code	CSB-PA017898LA01HU
Size	US$166
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Image	Western blot All lanes: PHF10 antibody at 8µg/ml Lane 1: Hela whole cell lysate Lane 2: 293T whole cell lysate Lane 3: A2780 whole cell lysate Lane 4: U251 whole cell lysate Lane 5: A549 whole cell lysate Lane 6: A375 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 57, 56, 52 kDa Observed band size: 57 kDa Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA017898LA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA017898LA01HU at dilution of 1:100 Immunofluorescent analysis of Hela cells using CSB-PA017898LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PHF10 Polyclonal antibody

Uniprot No.

Q8WUB8

Target Names

PHF10

Alternative Names

BAF45a antibody; BRG1-associated factor 45a antibody; FLJ10975 antibody; MGC111009 antibody; PHD finger protein 10 antibody; PHD finger protein 10; isoform a antibody; PHD zinc finger protein XAP135 antibody; Phf10 antibody; PHF10_HUMAN antibody; XAP135 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human PHD finger protein 10 protein (151-360AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The PHF10 Antibody (Product code: CSB-PA017898LA01HU) is Non-conjugated. For PHF10 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA017898LB01HU	PHF10 Antibody, HRP conjugated	ELISA
FITC	CSB-PA017898LC01HU	PHF10 Antibody, FITC conjugated
Biotin	CSB-PA017898LD01HU	PHF10 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Involved in transcription activity regulation by chromatin remodeling. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.

Gene References into Functions

We studied the expression of S100A4 and the total PHF10 protein in some human cancer cell lines. We have found that, in the cell lines studied, the PHF10 expression is correlated with the S100A4 expression. PMID: 27193724
Phosphorylation of PHF10 isoforms occurs while they are incorporated as a subunit of the PBAF complex, and therefore phosphorylation of PHF10 isoforms may play an essential role in regulation of PBAF complex's function and mechanism of action. PMID: 27239853
The study demonstrates the existence of functionally different PBAF complexes in mammalian cell. It also provides an insight into the molecular structure and role of human PHF10/BAF45a and characterizes it as an essential PBAF subunit. PMID: 24763304
PHF10 repressed caspase-3 expression and impaired the programmed cell death pathway in human gastric cancer at the transcriptional level. PMID: 20530714
Data show that that PHF10 is required for cell growth. PMID: 20068294

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Subcellular Location

Nucleus.

Protein Families

SAYP family

Database Links

HGNC: 18250

OMIM: 613069

KEGG: hsa:55274

STRING: 9606.ENSP00000341805

UniGene: Hs.435933

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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