PNPO Antibody

Datasheet

Code	CSB-PA885740LA01HU
Size	US$166
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Image	IHC image of CSB-PA885740LA01HU diluted at 1:600 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA885740LA01HU diluted at 1:600 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA885740LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PNPO Polyclonal antibody

Uniprot No.

Q9NVS9

Target Names

PNPO

Alternative Names

EC 1.4.3.5 antibody; FLJ10535 antibody; PDXPO antibody; PNPO antibody; PNPO_HUMAN antibody; Pyridoxal 5' phosphate synthase antibody; pyridoxamine 5' phosphate oxidase antibody; Pyridoxamine phosphate oxidase antibody; Pyridoxamine-phosphate oxidase antibody; pyridoxine 5' phosphate oxidase antibody; Pyridoxine-5'-phosphate oxidase antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Pyridoxine-5\\\'-phosphate oxidase protein (114-207AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The PNPO Antibody (Product code: CSB-PA885740LA01HU) is Non-conjugated. For PNPO Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA885740LB01HU	PNPO Antibody, HRP conjugated	ELISA
FITC	CSB-PA885740LC01HU	PNPO Antibody, FITC conjugated
Biotin	CSB-PA885740LD01HU	PNPO Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:500-1:1000
IF	1:200-1:500

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP).

Gene References into Functions

TGF-beta1-mediated PNPO expression was at least in part through the upregulation of miR-143-3p in epithelial ovarian cancer. PMID: 29238081
data support a pathogenic role of the c.347G>A (p.Arg116Gln) mutation in PNPO deficiency; the later onset of symptoms and the milder epilepsy phenotype of these expand the disease phenotype PMID: 28818555
Exome sequencing revealed that the patient was compound heterozygous for pathogenic mutations [c.546+1G>A (IVS5+1 G>A) and c.620delG (p.G207VfsX215)] in the PNPO gene. PMID: 26535729
One sequence variant, R116Q, a single nucleotide polymorphism that has been reported in the general population, was found to have an effect on PNPO activity. PMID: 24645144
Challenge to the paradigm of exclusive PLP responsiveness in patients with pyridoxal 5'-phosphate oxidase deficiency and underlines the importance of consecutive testing of pyridoxine and PLP in neonates with antiepileptic drug-resistant seizures. PMID: 24658933
Generalized epilepsies implicates susceptibility genes: CHRM3 at 1q43, VRK2 at 2p16.1, ZEB2 at 2q22.3, SCN1A at 2q24.3 and PNPO at 17q21.32. PMID: 22949513
provide evidence that a single arginine residue of the C terminus of pyridoxal 5'-phosphate synthase is responsible for coordinating co-operativity in this elaborate protein machinery PMID: 21283685
Results describe a combined computer and biochemical approach to characterize human pyridoxine 5'-phosphate oxidase (PNPO). PMID: 15182361
several mutations appear to cause epilepsy PMID: 15772097
We examined 8 single nucleotide polymorphisms (SNPs) in PNPO and its 5'-flanking regions in 359 schizophrenia patients and 582 control subjects. PMID: 17851041
Sequencing of the PNPO gene revealed a novel homozygous nonsense mutationt in exon 3 in the neonatal and infantile seizure. PMID: 18485777
PNP oxidase R229W mutation identified in patients with neonatal epileptic encephalopathy sgnificantly affect the catalytic efficiency of the enzyme. PMID: 19759001

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Involvement in disease

Pyridoxine-5'-phosphate oxidase deficiency (PNPOD)

Protein Families

Pyridoxamine 5'-phosphate oxidase family

Database Links

HGNC: 30260

OMIM: 603287

KEGG: hsa:55163

STRING: 9606.ENSP00000225573

UniGene: Hs.631742

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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