POFUT2 Antibody

Datasheet

Code	CSB-PA837737LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: U87 whole cell lysate All lanes: POFUT2 antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 49, 45 kDa Observed band size: 50 kDa IHC image of CSB-PA837737LA01HU diluted at 1:300 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA837737LA01HU diluted at 1:300 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) POFUT2 Polyclonal antibody

Uniprot No.

Q9Y2G5

Target Names

POFUT2

Alternative Names

POFUT2 antibody; C21orf80 antibody; FUT13 antibody; KIAA0958 antibody; GDP-fucose protein O-fucosyltransferase 2 antibody; EC 2.4.1.221 antibody; Peptide-O-fucosyltransferase 2 antibody; O-FucT-2 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human GDP-fucose protein O-fucosyltransferase 2 protein (125-271AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The POFUT2 Antibody (Product code: CSB-PA837737LA01HU) is Non-conjugated. For POFUT2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA837737LB01HU	POFUT2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA837737LC01HU	POFUT2 Antibody, FITC conjugated
Biotin	CSB-PA837737LD01HU	POFUT2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in the consensus sequence C1-X(2,3)-S/T-C2-X(2)-G of thrombospondin type 1 repeats where C1 and C2 are the first and second cysteines, respectively. O-fucosylates members of several protein families including the ADAMTS family, the thrombosporin (TSP) and spondin families. The O-fucosylation of TSRs is also required for restricting epithelial to mesenchymal transition (EMT), maintaining the correct patterning of mesoderm and localization of the definite endoderm. Required for the proper secretion of ADAMTS family members such as ADAMSL1 and ADAMST13.

Gene References into Functions

This study demonstrated that the alteration of POFUT2 expression in the superior temporal gyrus of elderly patients with schizophrenia. PMID: 27773385
POFUT2 and B3GLCT mediate a noncanonical endoplasmic reticulum quality-control mechanism that recognizes folded thrombospondin type 1 repeats and stabilizes them by glycosylation. PMID: 25544610
Structure of human POFUT2: insights into thrombospondin type 1 repeat fold and O-fucosylation. PMID: 22588082

Subcellular Location

Endoplasmic reticulum. Golgi apparatus. Note=Mainly located in the endoplasmic reticulum.

Protein Families

Glycosyltransferase 68 family

Tissue Specificity

Isoform A is expressed in fetal liver and peripheral blood lymphocytes. Isoform B is expressed in spleen, lung, testis, bone marrow, thymus, pancreas, prostate, fetal brain, fetal liver and fetal kidney. Isoform C is expressed in brain, heart, spleen, liv

Database Links

HGNC: 14683

OMIM: 610249

KEGG: hsa:23275

STRING: 9606.ENSP00000339613

UniGene: Hs.592164

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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