POU2F1 Antibody

Code CSB-PA018393LA01HU
Size US$299
Image
  • Immunofluorescence staining of Hela cells with CSB-PA018393LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) POU2F1 Polyclonal antibody
Uniprot No. P14859
Target Names POU2F1
Alternative Names FLJ42836 antibody; NF A1 antibody; NF-A1 antibody; Oct 1 antibody; Oct 1B antibody; Oct-1 antibody; OCT1 antibody; Octamer binding protein 1 antibody; Octamer binding transcription factor 1 antibody; Octamer-binding protein 1 antibody; Octamer-binding transcription factor 1 antibody; OTF 1 antibody; OTF-1 antibody; OTF1 antibody; OTTHUMP00000032348 antibody; OTTHUMP00000032350 antibody; OTTHUMP00000032351 antibody; PO21 antibody; PO2F1 antibody; PO2F1_HUMAN antibody; POU class 2 homeobox 1 antibody; POU domain class 2 transcription factor 1 antibody; POU domain; class 2; transcription factor 1 antibody; POU2F1 antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human POU domain, class 2, transcription factor 1 protein (442-743AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, IF
Recommended Dilution
Application Recommended Dilution
IF 1:50-1:200
Protocols ELISA Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity). In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.
Gene References into Functions
  1. E6/E7-p53-POU2F1-CTHRC1 axis promotes cervical cancer cell invasion and metastasis PMID: 28303973
  2. our results indicate that POU2F1 over-expression is positively associated with aggressive phenotypes and poor survival in patients with HCC, and POU2F1 regulated by AKT pathway promotes HCC aggressive phenotypes by regulating the transcription of EMT genes. PMID: 28489585
  3. A significant influence of OCT1 mRNA expression on the clinical outcome of the disease was observed. PMID: 28025785
  4. Children with certain OCT1 genotypes have higher risk for respiratory depression and nausea and vomiting, postoperatively, following morphine administration. PMID: 28470102
  5. High POU2F1 expression is associated with liver cancer. PMID: 26375440
  6. THE results of this investigation demonstrate that genetic polymorphisms in the hepatic uptake transporter OCT1 affect the pharmacokinetics and efficacy of tramadol. PMID: 27541716
  7. Hepatic distribution of metformin was significantly reduced after oral administration in individuals with genetic polymorphisms in OCT1. PMID: 28380657
  8. We found that a combination of two polymorphisms in hOCT1 that seem to play the role of predictor for imatinib in both efficacy and toxicity PMID: 28289867
  9. hOCT1 mRNA expression may serve as a clinical biomarker of response to imatinib and could be useful to predict Imatinib mesylate therapy outcome of chronic myeloid leukemia patients PMID: 28286932
  10. Our study demonstrates that the existence of Oct-1 isoforms which vary in length and sequence at the N-terminus of the protein, also contributes in surprising diversity of Oct-1 functions in transcription regulation. PMID: 27407111
  11. HLA-C Level Is Regulated by a Polymorphic Oct1 Binding Site in the HLA-C Promoter Region PMID: 27817866
  12. OCT1 transported sumatriptan with high capacity and sumatriptan uptake into human hepatocytes was strongly inhibited by the OCT1 inhibitor MPP(+) PMID: 26659468
  13. Oct-1 modifies S100A4 exchange between intra- and extracellular compartments in Namalwa cells and increases their sensitivity to glucocorticoids. PMID: 27096393
  14. Low expression of POU2F1 is associated with osteosarcoma. PMID: 27658774
  15. Data indicate that octamer transcription factor 1 (OCT1; pou2f1) expression levels are significantly upregulated in colorectal cancer. PMID: 26433389
  16. Comparative analysis of various tumor lines showed no clear correlation between the expression level of Mts1/S100A4 and the content of Oct-1. However, at stable transfection of tumor cells with Oct-1A, Oct-1L, and Oct-1X isoforms we detected an elevated level of Oct-1, which stimulated Mts1/S100A4 secretion. PMID: 27193714
  17. The response in Burkitt's lymphoma cells (Namalwa line) to camptothecin and dexamethasone of the Oct-1 target genes involved in the development of apoptosis depended, firstly, on the type of drug, secondly, on the concentration of Oct-1 in cells and, thirdly, on the Oct-1 isoform with which these cells were transfected PMID: 27599509
  18. Role of transcription factor Oct-1 in the regulation of expression of genes of the JAK-STAT signaling pathway in the Namalwa Burkitt's lymphoma cell line: overexpression of Oct-1 isoforms (Oct-1A, Oct-1L, and Oct-1X) causes a decrease in the activity of four genes involved in the JAK-STAT signaling pathway-IFNAR2, STAT1, STAT2, and STAT4; STAT2 and STAT4 are direct targets for Oct-1 protein PMID: 27417729
  19. single-nucleotide polymorphisms of OCT1 have selective effects on the glycemic response to metformin PMID: 26464716
  20. OCT-1 plays a critical role in CD4 T cells through regulating gene expression during differentiation and mediating long-range chromosomal interactions. (Review) PMID: 27126747
  21. Oct-1 is a direct link of transcription factors with the core transcriptional machinery and chromatin remodeling and, depending on other factors present in particular cell types, it can direct cell type specific regulation. (Review) PMID: 27063953
  22. levels of microRNA-451 (miR-451) are negatively regulated through the phosphorylation and inactivation of its direct transcriptional activator OCT1 by 5' AMP-activated protein kinase PMID: 25937278
  23. hOCT1 c.480C>G SNP may significantly influence imatinib pharmacokinetics, supporting further analyses in larger groups of patients PMID: 24589908
  24. These results suggest that reduced OCT1 transport is an important determinant of metformin intolerance. PMID: 25510240
  25. POU2F1 activity regulates HOXD10 and HOXD11 gene expression in head and neck squamous cell carcinoma, promoting a proliferative and invasive phenotype. PMID: 25301728
  26. These experiments suggested that some human herpesvirus 8-infected PEL cell line-specific DNA-binding factors, such as OCT-1, should be involved in the upregulation of ANGPT-1 in a sequence-dependent manner. PMID: 25631079
  27. we demonstrate that the Oct-1 transcription factor, commonly considered pro-proliferative, indeed facilitates IFN-gamma induced apoptosis in 5637 bladder carcinoma cells PMID: 25236570
  28. combination of prelamin A accumulation and stress conditions enhance the aging phenotype by dysregulating the activity of the octamer binding protein Oct-1 PMID: 24753226
  29. a differential control exerted by both Oct-1 and Oct-2 in the transcriptional regulation of the HMGA1 gene PMID: 24367622
  30. T3 throid hormone enhanced the recruitment of the TRbeta1/Oct-1 complex on Octamer-transcription factor-1 site within cyclin D1 promoter. PMID: 24121026
  31. OCT-1 overexpression may be a marker for poor prognosis in patients with well-differentiated gastric adenocarcinoma. PMID: 24566898
  32. The new isoform of Oct-1 transcription factor is transcribed from alternative promoter. PMID: 24466753
  33. a new alternative promoter in the gene of the oct-1 transcription factor PMID: 23657650
  34. Data suggest that Oct1/Pou2f1 integrates metabolic and stress signals via O-GlcNAc modification to regulate target gene activity. PMID: 23580612
  35. our results did not show any pathogenic HOXA1 mutation, our results suggest that VSD might not be a clinically isolated manifestation of HOXA1 mutations. PMID: 22777240
  36. This study shows that long-term exposure to cisplatin promotes methylation of the OCT1 gene in human esophageal cancer cells, which in turn results in cisplatin resistance. PMID: 23053895
  37. Results indicate that lamin B1 (LMNB1) accumulation in adult-onset autosomal dominant leukodystrophy (ADLD) is associated with Oct-1 recruitment. PMID: 23261988
  38. STAT3 regulates the transcription and expression of Oct-1 by directly targeting its promoter. PMID: 23172665
  39. Oct-1 acts as a transcriptional repressor of C-reactive protein expression and it does so by occupying its cognate site on the promoter and also via other transcription factors by an as yet undefined mechanism PMID: 22750226
  40. Overexpression of wild-type, but not OCT1 loss-of-function variants, significantly increased tropisetron uptake. Correspondingly, patients with two loss-of-function OCT1 alleles had higher tropisetron plasma concentrations. PMID: 20921968
  41. AK inhibitor MK-0457 induces the growth arrest DNA damage-inducible (Gadd) 45a through recruitment of octamer-binding (Oct)-1 transcription factor at a critical promoter region for gene transcription and covalent modifications of histone H3. PMID: 22521726
  42. Exogenous Oct-1 expression showed a concentration-dependent activation of U6 promoter that was further stimulated by the p38 kinase inhibitors. PMID: 22310390
  43. Sox2 modulates the mechanism of translocation of Oct1. Both Sox2 and the Oct1 POU(HD) domain are transiently released from the specific ternary complex by sliding to an adjacent nonspecific site, followed by direct transfer to another DNA molecule PMID: 22396547
  44. patients with high immunoreactivity of Oct1 showed a low cancer-specific survival rate. PMID: 21387309
  45. role for Oct1 in mitosis PMID: 21897860
  46. Oct-1 is downregulated by reactive oxygen species via CpG island methylation in its promoter PMID: 21985966
  47. antioxidative response element-like/Oct-1 binding site was noticed to be essential for shear stress-dependent downregulation of Nox4 PMID: 21399967
  48. The authors demonstrate that the cellular transcription factor Oct-1 cooperates with the EBV immediate-early protein BRLF1 (R, Rta) to induce lytic viral reactivation. PMID: 21697476
  49. Genetic polymorphisms of the OCT1 is associated with the response to imatinib in patients newly diagnosed with chronic-phase chronic myeloid leukemia PMID: 21185600
  50. High OCT-1 is associated with chronic myeloid leukemia. PMID: 20971815
  51. analysis of Oct1 characterized by liquid crystal and paramagnetic NMR PMID: 21555551
  52. polymorphisms in OCT1 influence the pharmacokinetics of O-desmethyltramadol, presumably by affecting its uptake into liver cells PMID: 21562485
  53. The findings invoke a model wherein incomplete DNA synthesis during S phase leads to a DNA damage response and formation of 53BP1-OPT domains in the subsequent G1. PMID: 21444690
  54. the single nucleotide polymorphism rs1046295 of PHF11 modulates allele-specific binding by the octamer-binding transcription factor 1 PMID: 21320718
  55. Using separate cohorts, we observed consistent results showing the contribution of multiple variants at POU2F1 to the risk of Type 2 diabetes. PMID: 21059098
  56. Increased OCT1 gene expression in adipose tissue of obese subjects may contribute to increased metformin action in these subjects. PMID: 20956498
  57. The interplay between Oct-1 and unknown transcription factors may be responsible for the IL-4 C-33T polymorphism effects. PMID: 19804407
  58. Disrupting the bindings of the Oct-1 transcriptional factors with the decoy oligonucleotide provides a novel approach for inducing expression of the gamma-globin genes. PMID: 19327156
  59. HMGB2 potentiates GATA-1-dependent transcription of GFI1B by Oct-1 and thereby controls erythroid differentiation. PMID: 19965638
  60. Results demonstrate that functional interaction of Oct-1 and Brn-1 with androgen receptors is determined by the precise sequence of the octamer binding site, and by differential interaction with transcriptional machinery. PMID: 11997177
  61. Oct-1 potentiates CREB-dependent cyclin D1 transcriptional activity by a phospho-CREB and CREB binding protein-independent mechanism PMID: 12391146
  62. results clearly indicate a role of octamer transcription factor-1 in the transcriptional repression of the human gonadotropin-releasing hormone receptor gene PMID: 12446597
  63. Our data on the interaction of two promoters with the enhancer and silencer in different cell types point to fine tissue-specific regulation of the oct-1 gene expression, especially in lymphatic cells. PMID: 12669425
  64. Selective inhibition of class switching to IGG and IgE by recruitment of this and HOXC4 proteins and Ku70/ku86 to newly identified ATTT cis-elements. PMID: 12672812
  65. enhancement of transcriptional potential by OBF1 PMID: 12727885
  66. transcription of the Oct-1 gene is regulated by two promoters located upstream of the exon 1U and upstream of the exon 1L PMID: 12853155
  67. The Oct-1 protein has been isolated,purified and characterized functionally. Histone gene expression is dependent on OCT1 and OCA-S coactivator complex. PMID: 12887915
  68. OCA-S is a multicomponent OCT1 coactivator which has been isolated and functionally characterized. PMID: 12887926
  69. OCT1 and SOX2 have roles in transcriptional activation of the Oct1.Sox2.Hoxb1-DNA ternary transcription factor complex PMID: 14559893
  70. Results suggest a mechanism for the regulation of Oct-1 in response to DNA damage through specific phosphorylation within the NH(2)-terminal transcriptional regulatory domain. PMID: 14612514
  71. transcriptional regulation of the key cell cycle regulator cyclin D1 and roles of both STAT5 and Oct-1 in this process PMID: 14645506
  72. Data show that Oct-1 occupies the endogenous HLA-DRA promoter when the HLA-DRA promoter is inactive in retinoblastoma protein-defective cells. PMID: 15105429
  73. Oct-1 and the TALE homeodomain proteins have roles in regulating the expression of the GnRH gene in neurons PMID: 15138251
  74. a multiprotein complex containing GATA-1, Oct-1, and other protein factors may contribute to the formation of a repressive chromatin structure that silences gamma-globin gene expression PMID: 15613485
  75. OCT-1 binds within DHS3 to silence Fshr transcription, an activity that involves members of the GATA family PMID: 15817654
  76. physical binding to the family of repeats in Epstein-Barr virus oriP by the cellular transcription factors Oct-1 and Oct-2 was demonstrated by using an electrophoretic mobility-shift assay PMID: 15831936
  77. Data show that GRG proteins, including GRG5, interact with two regulators of GnRH transcription, the homeodomain proteins MSX1 and OCT1, and regulate GnRH promoter activity. PMID: 16002402
  78. identified an AHSP gene erythroid promoter with functionally important binding sites for GATA-1- and Oct-1-related proteins PMID: 16186125
  79. the binding of Oct-1 to the T-13,910 lactase variant directs increased lactase promoter activity and this might provide an explanation for the lactase persistence phenotype in the human population PMID: 16301215
  80. in the case of one non-small cell lung carcinoma line (NSCLC), Rb re-expression failed to rescue HLA-DRA inducibility despite successful re-establishment of Rb-function; this failure is traceable to the failure of Rb to rescue normal Oct-1 function PMID: 16360016
  81. POU5F1 and POU2F subfamily members play a pivotal role for the FZD5 expression in undifferentiated human ES cells, fetal liver/spleen, adult colon, pancreatic islet, and diffuse-type gastric cancer PMID: 17273778
  82. Oct-1 is an important transcriptional repressor for p15(INK4b) gene and the transcriptional repression of the p15(INK4b) gene by Oct-1 may be one of the regulatory mechanisms of cellular senescence. PMID: 17316622
  83. The transcription factors Oct-1 and -2 inhibit and enhance, respectively, the expression of exon 1-containing transcripts and CCR5 surface levels. PMID: 17442950
  84. The cytotoxicity of oxaliplatin was suggested to be affected by the levels of ATP7A and hOCT1 mRNAs. PMID: 18030470
  85. Suggest that Oct-1 may play an important role in the development of primary lower extremity varicose veins. PMID: 18083338
  86. Oct-1 transactivates hPTTG1, and both proteins are concordantly overexpressed in endocrine tumors. PMID: 18550719
  87. Testosterone represses MAFbx expression via interactions of the AR with Oct-1. PMID: 18599544
  88. A functional role of PPARgamma/RXRalpha and Oct-1 in the regulation of the FABP2 gene. PMID: 18634911
  89. A redox-modulated direct p38/GAPDH-Oct-1 interaction nucleates the occupancy of the H2B promoter by the OCA-S complex, in which p36/LDH plays a critical role in the hierarchical organization of the complex. PMID: 18682386
  90. intersegmental transfer involves a ternary intermediate, or transition state in which the DNA-binding domains bridge two different DNA fragments simultaneously PMID: 18772384
  91. Oct1 may have regulatory functions in prostate development and cancer progression. PMID: 19058140
  92. O-linked N-acetylglucosamines within the second serine/threonine-rich region of Sp1 interrupts a known interaction between Sp1 and Oct1 PMID: 19070619
  93. Oct-1 is involved in the regulation of CDKN1A in reponse to clinically relevant doses of ionizing radiation. PMID: 19118657
  94. we show that association of Oct4 and Oct1 with a distinct group of in vivo targets is inducible by stress, and that Oct1 is essential for a normal post-stress transcriptional response PMID: 19171782
  95. Observations identify Oct-1 as a transcriptional repressor of ChREBP and suggest that insulin stimulates ChREBP expression via attenuating the repressive effect of Oct-1. PMID: 19359385
  96. Results discuss how constitutively bound factors such as NF1 and Oct1 may participate in the maintenance of tissue specificity of genes responsive to hormones through receptors such as the glucocorticoid receptor. PMID: 19463811
  97. Data show that elevation of cAMP leads to enhanced phosphorylation and nuclear exclusion of Oct-1 and reduced interactions between Oct-1 or nuclear co-repressors and the Cdx-2 gene promoter. PMID: 19617623

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Subcellular Location Nucleus
Protein Families POU transcription factor family, Class-2 subfamily
Tissue Specificity Ubiquitous. Isoform 2 is lymphocyte-specific.
Database Links

HGNC: 9212

OMIM: 164175

KEGG: hsa:5451

STRING: 9606.ENSP00000356840

UniGene: Hs.283402

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