POU2F1 Antibody

Datasheet
Code CSB-PA018393LA01HU
See More Details Free Antibody trial simple
Size US$299
Image
  • Immunofluorescence staining of Hela cells with CSB-PA018393LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Product Details

Full Product Name Rabbit anti-Human POU2F1 Polyclonal antibody
Uniprot No. P14859
Target Names POU2F1
Alternative Names POU domain, class 2, transcription factor 1 (NF-A1) (Octamer-binding protein 1) (Oct-1) (Octamer-binding transcription factor 1) (OTF-1), POU2F1, OCT1 OTF1
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human POU domain, class 2, transcription factor 1 protein (442-743AA)
Immunogen Species Human
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, IF
Recommended Dilution
Application Recommended Dilution
IF 1:50-1:200
Protocols ELISA Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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Target Data

Function Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity). In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.
Subcellular Location Nucleus
Protein Families POU transcription factor family, Class-2 subfamily
Tissue Specificity Ubiquitous. Isoform 2 is lymphocyte-specific.
Database Links

HGNC: 9212

OMIM: 164175

KEGG: hsa:5451

STRING: 9606.ENSP00000356840

UniGene: Hs.283402

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