Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PSMB5 Polyclonal antibody

Uniprot No.

P28074

Target Names

PSMB5

Alternative Names

DKFZp459C139 antibody; EC 3.4.25.1 antibody; LMPX antibody; Macropain epsilon chain antibody; MB1 antibody; MGC104214 antibody; MGC118075 antibody; MGC134464 antibody; Multicatalytic endopeptidase complex epsilon chain antibody; Proteasome (prosome, macropain) subunit, beta type, 5 antibody; Proteasome beta 5 subunit antibody; Proteasome catalytic subunit 3 antibody; Proteasome chain 6 antibody; Proteasome epsilon chain antibody; Proteasome subunit beta type-5 antibody; Proteasome subunit MB1 antibody; Proteasome subunit X antibody; Proteasome subunit, beta type, 5 antibody; Proteasome subunit, beta-5 antibody; PSB5_HUMAN antibody; PSMB5 antibody; PSX large multifunctional protease X antibody; X antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Proteasome subunit beta type-5 protein (106-188AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

HRP

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Background

Function

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Within the 20S core complex, PSMB5 displays a chymotrypsin-like activity.

Gene References into Functions

Our results suggest a potential role for PSMB5 as a biomarker and therapeutic target for Triple-negative breast cancer PMID: 28623645
Results identified PSMB5 Q62P mutation to underlie bortezomib resistance in Down-syndrome-associated acute myeloid leukemia cells. PMID: 28143565
Data indicate that proteasomal subunit X PSMB5 is a target of signal transducer and activator of transcription 3 (STAT3). PMID: 24627483
Nuclear MB1 expression was an independent predictor of worse survival in ovarian cancer. PMID: 19243813
critical role of PSMB5 in 20S proteasome-mediated protection against replicative senescence, pointing to a possible strategy for maintaining the integrity of culture-expanded bone marrow stromal cells by manipulating the expression of PSMB5 PMID: 24393841
The expression of proteasome beta5 decreases markedly in human atherosclerotic plaques. PMID: 22781773
Data indicate that treatment-emergent resistance to single-agent bortezomib was independent of variants in the proteasome genes PSMB1, PSMB5, PSMB6, PSMB8, PSMB9, and PSMB10. PMID: 23018640
Letter/Case Reports: proteasome subunit beta5t is expressed in cervical ectopic thymoma. PMID: 22523338
PSMB5 overexpression is associated with Bortezomib resistance in myeloma. PMID: 21978467
No mutations or differences in PSMB5 mRNA expression were seen before bortezomib treatment in 3 multiple myeloma patients, but after treatment, 1 patient showed PSMB5 upregulation associated with bortezomib resistance. PMID: 21920470
CDK5 regulation of proteasome subunit PSMB5 was identified as a probable route to antineoplastic drug sensitization. PMID: 21289309
Expression of mutated PSMB5 was associated with the prevention of the accumulation of unfolded proteins PMID: 20555361
interaction of TAP1 and TAP2 and P-glycoprotein with proteasome subunits beta-5 and beta-5i suggest direct targeting of antigenic peptides to the ER via a TAP-proteasome association and a possible role for P-glycoprotein PMID: 15488952
Proteasome activity can be genetically "upregulated" in lens cells by overexpression of beta5 catalytic subunit. Resulting increase in proteasome activity leads to decrease in oxidized proteins and enhanced cell survival following oxidative stress. PMID: 17262013
G322A mutation of the PSMB5 gene is a novel mechanism for bortezomib resistance. PMID: 18502982
nonsynonymous coding single nucleotide polymorphismsin the proteasome beta 5 subunit gene did not show significant effects on proteasome activity, but SNPs did influence transcription PMID: 18519783
Overexpression of PSMB5 is an important mechanism for bortezomib resistance in Jurkat cell lines. PMID: 18562081
a novel mechanism of bortezomib resistance associated with the selective overexpression of a mutant PSMB5 protein PMID: 18565852
Mutations of the PSMB5 gene resulting in substitutions of Ala49 and Ala50 of PSMB5 protein can confer varying bortezomib resistance PMID: 19426847