PTCH2 Antibody

Datasheet

Code	CSB-PA896928LA01HU
Size	US$166
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Image	IHC image of CSB-PA896928LA01HU diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of 293 cells with CSB-PA896928LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PTCH2 Polyclonal antibody

Uniprot No.

Q9Y6C5

Target Names

PTCH2

Alternative Names

patched (Drosophila) homolog 2 antibody; patched homolog 2 (Drosophila) antibody; Protein patched homolog 2 antibody; PTC2 antibody; PTC2_HUMAN antibody; Ptch2 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Protein patched homolog 2 protein (793-951AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The PTCH2 Antibody (Product code: CSB-PA896928LA01HU) is Non-conjugated. For PTCH2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA896928LB01HU	PTCH2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA896928LC01HU	PTCH2 Antibody, FITC conjugated
Biotin	CSB-PA896928LD01HU	PTCH2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Plays a role in the control of cellular growth. May have a role in epidermal development. May act as a receptor for Sonic hedgehog (SHH).

Gene References into Functions

Combined heterozygous germline mutations in PTCH1 and PTCH2 were identified in a patient with embryonal rhabdomyosarcoma. PMID: 29230040
Frameshift mutation in the PTCH2 gene can cause nevoid basal cell carcinoma syndrome. PMID: 23479190
PTCH2 isoforms have distinct roles in Hedgehog signalling. PMID: 14613484
PTCH2 (2157G-->A), a novel missense mutation, underlies NBCCS, resulting in the loss of PTCH2 inhibitory function in the Shh signalling pathway. PMID: 18285427
A susceptibility locus on 1p32-1p34 for congenital macrostomia in a Chinese family and identification of a novel PTCH2 mutation are reported. PMID: 19208383

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Involvement in disease

Medulloblastoma (MDB); Basal cell carcinoma (BCC)

Subcellular Location

Membrane; Multi-pass membrane protein.

Protein Families

Patched family

Database Links

HGNC: 9586

OMIM: 155255

KEGG: hsa:8643

STRING: 9606.ENSP00000361266

UniGene: Hs.591497

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Hedgehog signaling pathway

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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