Phospho-FBXL3 (Ser320) Antibody

Code CSB-PA976364
Size US$360
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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) FBXL3 Polyclonal antibody
Uniprot No.
Target Names
FBXL3
Alternative Names
F box and leucine rich repeat protein 3 antibody; F box and leucine rich repeat protein 3A antibody; F box protein Fbl3a antibody; F-box and leucine-rich repeat protein 3A antibody; F-box/LRR-repeat protein 3 antibody; F-box/LRR-repeat protein 3A antibody; FBL3 antibody; FBL3A antibody; Fbxl3 antibody; FBXL3_HUMAN antibody; FBXL3A antibody
Raised in
Rabbit
Species Reactivity
Humann,Mouse,Rat
Immunogen
Peptide sequence around phosphorylation site of serine 320 (S-V-S(p)-K-D) derived from Human FBXL3.
Immunogen Species
Homo sapiens (Human)
Clonality
Polyclonal
Purification Method
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Concentration
It differs from different batches. Please contact us to confirm it.
Form
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Tested Applications
ELISA,WB
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:1000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Substrate-recognition component of the SCF(FBXL3) E3 ubiquitin ligase complex involved in circadian rhythm function. Plays a key role in the maintenance of both the speed and the robustness of the circadian clock oscillation. The SCF(FBXL3) complex mainly acts in the nucleus and mediates ubiquitination and subsequent degradation of CRY1 and CRY2. Activity of the SCF(FBXL3) complex is counteracted by the SCF(FBXL21) complex.
Gene References into Functions
  1. data indicate an oncogenic role of miR-181d in CRC by promoting glycolysis, and miR-181d/CRY2/FBXL3/c-myc feedback loop might be a therapeutic target for patients with CRC. PMID: 28749470
  2. These results demonstrate that CRY2 stability controlled by FBXL3 plays a key role in the regulation of human sleep wake behavior. PMID: 27529127
  3. CRY2 and FBXL3 cooperatively degrade c-MYC preventing the development of cancer. PMID: 27840026
  4. Data show that CRISPR/Cas9 genome editing generated F-box and leucine-rich repeat protein 3 (Fbxl3) knockout in circadian clock model U2OS cells. PMID: 26243628
  5. Substrate binding promotes formation of the Skp1-Cul1-Fbxl3 (SCF(Fbxl3)) protein complex. PMID: 24085301
  6. Mutation in the Fbxl3 protein reveals distinct roles for CRY1 and CRY2 in the circadian molecular clockwork. PMID: 23616524
  7. crystal structures of mammalian CRY2 in its apo, FAD-bound and FBXL3-SKP1-complexed forms PMID: 23503662
  8. study demonstrates that the SCF(Fbxl3)ubiquitin ligase controls the oscillations of the circadian clock by mediating the degradation of Cry1 and Cry2 proteins PMID: 17463251

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Subcellular Location
Nucleus. Cytoplasm. Note=Predominantly nuclear.
Tissue Specificity
Widely expressed.
Database Links

HGNC: 13599

OMIM: 605653

KEGG: hsa:26224

STRING: 9606.ENSP00000347834

UniGene: Hs.508284

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