Phospho-NIFK (T234) Antibody

Datasheet

Code	CSB-PA070019
Size	US$119
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

Q9BYG3

Target Names

NIFK

Alternative Names

hNIFK antibody; MK67I_HUMAN antibody; MKI67 FHA Domain Interacting Nucleolar Phosphoprotein antibody; MKI67 FHA domain-interacting nucleolar phosphoprotein antibody; mki67ip antibody; NOPP34 antibody; Nucleolar phosphoprotein Nopp34 antibody; Nucleolar protein interacting with the FHA domain of pKI 67 antibody; Nucleolar protein interacting with the FHA domain of pKI-67 antibody; pKi67 antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse

Immunogen

Synthesized peptide derived from Human NIFK around the phosphorylation site of T234.

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Form

Liquid

Tested Applications

IHC, IF, ELISA

Recommended Dilution

Application	Recommended Dilution
IHC	1:100-1:300
IF	1:200-1:1000
ELISA	1:40000

Protocols

Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Gene References into Functions

NIFK is required for lung cancer progression via the RUNX1-dependent CK1alpha repression, which activates TCF4/beta-catenin signaling in metastasis and the Ki-67-dependent regulation in cell proliferation. PMID: 26984280
The RNA recognition motif of NIFK is required for rRNA maturation during cell cycle progression. PMID: 25826659
Potential encounter complexes of the Ki67FHA receptor and hNIFK peptide are misregistered states of the beta-sheet. PMID: 21539773
nmr analysis of the solution structure of the FHA domain of human Ki67 and mapping of the binding surface for NIFK binding PMID: 14659764
Better basal gene expression was observed by co-cultured respiratory epithelial cells compared to dispase dissociated cells. PMID: 17891046

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Subcellular Location

Nucleus, nucleolus. Chromosome. Note=Localizes to mitotic chromosomes in conjunction with MKI67.

Database Links

HGNC: 17838

OMIM: 611970

KEGG: hsa:84365

STRING: 9606.ENSP00000285814

UniGene: Hs.367842

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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