Phospho-RAPGEF1 (Y504) Antibody

Code CSB-PA008953
Size US$100
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Product Details

Uniprot No.
Target Names
Alternative Names
C3G antibody; C3G protein antibody; CRK SH3 binding GNRP antibody; CRK SH3-binding GNRP antibody; DKFZp781P1719 antibody; GRF 2 antibody; GRF2 antibody; Guanine nucleotide releasing factor 2 (specific for crk proto oncogene) antibody; Guanine nucleotide releasing factor 2 antibody; Guanine nucleotide-releasing factor 2 antibody; OTTHUMP00000064558 antibody; Protein C3G antibody; Rap guanine nucleotide exchange factor (GEF) 1 antibody; Rap guanine nucleotide exchange factor 1 antibody; RAPGEF 1 antibody; RAPGEF1 antibody; RPGF1_HUMAN antibody
Raised in
Species Reactivity
Synthesized peptide derived from Human C3G around the phosphorylation site of Y504.
Immunogen Species
Homo sapiens (Human)
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
It differs from different batches. Please contact us to confirm it.
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Tested Applications
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
ELISA 1:10000
Troubleshooting and FAQs
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS. Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1. Plays a role in the establishment of basal endothelial barrier function. Plays a role in nerve growth factor (NGF)-induced sustained activation of Rap1 and neurite outgrowth.
Gene References into Functions
  1. our studies uncover novel mechanisms by which C3G controls key aspects of tumorigenesis. PMID: 27286263
  2. C3G/RAP1 activity is involved in the metastatic spread of epithelial ovarian cancer. PMID: 25617801
  3. C3G plays an important role in platelet clotting through a mechanism involving its GEF activity and suggest that it might be also involved in neutrophil development. PMID: 22659131
  4. The possibility of cellular phospho-C3G (pC3G) being a substrate of the intracellular T-cell protein tyrosine phosphatase TC-PTP (PTPN2) using the human neuroblastoma cell line, was studied. PMID: 21876762
  5. Lyn controls spatial activation of Rap1 by recruiting the CrkL-C3G protein complex to the leading edge PMID: 21628423
  6. found somatic demethylation of a relaxed-criterion CpG island (CGI-B) located in the first intron of RAPGEF1 in 40% of colon cancers and 8% of gastric cancers relative to their matching normal tissues that were always methylated PMID: 21399874
  7. Data demonstrated that the polymorphism in TP53 (rs1042522) was associated with type 2 diabetes, and that potential interaction of TP53 (rs1042522) and RAPGEF1 (rs11243444), or NRF1 (rs1882095) increased the risk of type 2 diabetes. PMID: 21146886
  8. significant positive correlation between layers II and IV of the dorso-lateral prefrontal cortex in the percentage of MR-GEF expressing neurons in individuals with bipolar disorder PMID: 20436929
  9. C3G overexpression induces neurite-like extensions in MDA-MB-231 and BT549 breast carcinoma cells and not in a variety of other cancer cell lines examined. PMID: 21223981
  10. C3G as a novel target of c-Abl PMID: 20581864
  11. ALK activation of Rap1 via the Rap1-specific GEF C3Gmay contribute to cell proliferation and oncogenesis of neuroblastoma. PMID: 20190816
  12. Cbl-b plays a negative role in Crk-L-C3G-mediated Rap1 and LFA-1 activation in T cells. PMID: 12697763
  13. C3G and Hck interact physically and functionally in vivo to activate kinase-dependent and caspase-mediated apoptosis, which is independent of catalytic domain of C3G PMID: 14551197
  14. C3G interferes with at least two separate aspects of oncogenic transformation - cell cycle progression and loss of contact inhibition. PMID: 15077165
  15. amplification and increased expression of the C3G gene may play some role in human lung carcinogenesis through derangement of the CRK-Rap1 signaling pathway PMID: 15138850
  16. Src family kinases or pervanadate treatment induces phosphorylation of C3G on Y504. Unlike C3G, which is mostly cytosolic, pY504C3G locates to the Golgi & subcortical actin cytoskeleton, providing evidence for a function for C3G at these compartments. PMID: 15320955
  17. Inactivation of Crk SH3 domain-binding guanine nucleotide-releasing factor is associated with cervical squamous cell carcinoma PMID: 16681758
  18. C3G triggers PP2A activation and binding to MEK and ERK at the subcortical actin cytoskeleton, thus favouring ERK dephosphorylation. PMID: 17825818
  19. Results identify a mechanism by which the WAVE2 complex regulates T cell receptor signaling to Rap1 and integrin activation via Abl- and CrkL-C3G. PMID: 18809728
  20. Rap1 and its exchange factor C3G in mediating Fc gammaR-dependent phagocytosis. PMID: 18832707
  21. Genetic polymorphisms in the RAPGEF1 gene and positive association between one polymorphism and type 2 diabetes in the Korean population. PMID: 19297053
  22. These results strongly suggest a dual regulatory role for C3G in chronic myeloid leukemia cells, modulating both apoptosis and survival via Rap-dependent and independent mechanisms. PMID: 19324082

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Subcellular Location
Early endosome.
Tissue Specificity
Ubiquitously expressed in adult and fetus. Expression is high in adult skeletal muscle and placenta and in fetal brain and heart. Low levels of expression in adult and fetal liver.
Database Links

HGNC: 4568

OMIM: 600303

KEGG: hsa:2889

STRING: 9606.ENSP00000361264

UniGene: Hs.127897

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