RAB26 Antibody

Datasheet

Code	CSB-PA892347LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Rat liver tissue All lanes: RAB26 antibody at 5.9μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 28, 22 kDa Observed band size: 28 kDa IHC image of CSB-PA892347LA01HU diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA892347LA01HU diluted at 1:200 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) RAB26 Polyclonal antibody

Uniprot No.

Q9ULW5

Target Names

RAB26

Alternative Names

CATX-8 antibody; RAB11c antibody; Rab26 antibody; RAB26 member RAS oncogene family antibody; RAB26_HUMAN antibody; Ras related oncogene protein antibody; Ras related protein Rab 26 antibody; Ras-associated protein Rab26 antibody; Ras-related protein Rab-26 antibody; V46133 antibody

Raised in

Rabbit

Species Reactivity

Human, Rat

Immunogen

Recombinant Human Ras-related protein Rab-26 protein (1-58AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The RAB26 Antibody (Product code: CSB-PA892347LA01HU) is Non-conjugated. For RAB26 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA892347LB01HU	RAB26 Antibody, HRP conjugated	ELISA
FITC	CSB-PA892347LC01HU	RAB26 Antibody, FITC conjugated
Biotin	CSB-PA892347LD01HU	RAB26 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Mediates transport of ADRA2A and ADRA2B from the Golgi to the cell membrane. Plays a role in the maturation of zymogenic granules and in pepsinogen secretion in the stomach. Plays a role in the secretion of amylase from acinar granules in the parotid gland.

Gene References into Functions

Rab26 selectively directs synaptic and secretory vesicles into preautophagosomal structures. PMID: 25643395
Increased RAB26 expression causes lysosomes to coalesce in a central, perinuclear region within the cell. PMID: 24413166
The small GTPase Rab26 regulates the Golgi to cell surface traffic of alpha(2)-adrenergic receptors, likely through a physical interaction. PMID: 23105096
MIST1 binds to highly conserved CATATG E-boxes to directly activate transcription of 6 genes, including those encoding the small GTPases RAB26 and RAB3D. PMID: 20038531

Subcellular Location

Golgi apparatus membrane; Lipid-anchor; Cytoplasmic side. Cytoplasmic vesicle, secretory vesicle membrane; Lipid-anchor; Cytoplasmic side.

Protein Families

Small GTPase superfamily, Rab family

Tissue Specificity

Predominantly expressed in brain.

Database Links

HGNC: 14259

OMIM: 605455

KEGG: hsa:25837

STRING: 9606.ENSP00000210187

UniGene: Hs.3797

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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