RANBP10 Antibody

Code CSB-PA764955LA01HU
Size US$166
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Image
  • Western Blot
    Positive WB detected in: SH-SY5Y whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate
    All lanes: RANBP10 antibody at 2.1μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 68, 59, 65 kDa
    Observed band size: 68 kDa

  • IHC image of CSB-PA764955LA01HU diluted at 1:200 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunoprecipitating RANBP10 in Rat brain tissue
    Lane 1: Rabbit control IgG instead of CSB-PA764955LA01HU in Rat brain tissue. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-PA764955LA01HU (6µg) + Rat brain tissue (1mg)
    Lane 3: Rat brain tissue (20µg)

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) RANBP10 Polyclonal antibody
Uniprot No.
Target Names
RANBP10
Alternative Names
FLJ31165 antibody; KIAA1464 antibody; RAN binding protein 10 antibody; Ran-binding protein 10 antibody; RanBP10 antibody; RBP10_HUMAN antibody
Raised in
Rabbit
Species Reactivity
Human, Rat
Immunogen
Recombinant Human Ran-binding protein 10 protein (3-165AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The RANBP10 Antibody (Product code: CSB-PA764955LA01HU) is Non-conjugated. For RANBP10 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA764955LB01HU RANBP10 Antibody, HRP conjugated ELISA
FITC CSB-PA764955LC01HU RANBP10 Antibody, FITC conjugated
Biotin CSB-PA764955LD01HU RANBP10 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IP
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:200-1:500
IP 1:200-1:2000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
May act as an adapter protein to couple membrane receptors to intracellular signaling pathways. Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1. Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but does not affect estrogen-induced transactivation. Acts as a guanine nucleotide exchange factor (GEF) for RAN GTPase. May play an essential role in hemostasis and in maintaining microtubule dynamics with respect to both platelet shape and function.
Gene References into Functions
  1. Authors provide evidence that enhancement of neuronal morphology through RANBP10 is one of the neuroprotective mechanisms for miR-196a. PMID: 28744327
  2. The results showed that ICP0 interacted with cell protein RanBP10, a known transcriptional coactivator, in human herpesvirus 1-infected cells. PMID: 26739050
  3. data support the idea that RanBP9 and RanBP10 may function as signaling integrators and dictate the efficient regulation of D(1) receptor signaling by PKCdelta and PKCgamma PMID: 20395553
  4. RanBP10 might serve as a molecular link between Ran and noncentrosomal microtubules PMID: 18347012
Subcellular Location
Cytoplasm, cytosol. Nucleus. Note=Predominantly cytoplasmic.
Protein Families
RANBP9/10 family
Tissue Specificity
Broadly expressed, with highest levels in skeletal muscle.
Database Links

HGNC: 29285

OMIM: 614031

KEGG: hsa:57610

STRING: 9606.ENSP00000316589

UniGene: Hs.368569

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