Name: RBM4 Antibody
Brand: CUSABIO
SKU: CSB-PA880118LA01HU

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) RBM4 Polyclonal antibody

Uniprot No.

Q9BWF3

Target Names

RBM4

Alternative Names

dkfzp547k0918 antibody; hLark antibody; lark antibody; Lark homolog antibody; lark homologue antibody; mgc75138 antibody; RBM4 antibody; rbm4 lark antibody; RBM4_HUMAN antibody; RNA binding motif 4 antibody; RNA binding motif 4a antibody; RNA-binding motif protein 4 antibody; RNA-binding motif protein 4a antibody; RNA-binding protein 4 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human RNA-binding protein 4 protein (121-205AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The RBM4 Antibody (Product code: CSB-PA880118LA01HU) is Non-conjugated. For RBM4 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA880118LB01HU	RBM4 Antibody, HRP conjugated	ELISA
FITC	CSB-PA880118LC01HU	RBM4 Antibody, FITC conjugated
Biotin	CSB-PA880118LD01HU	RBM4 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

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Applications : WB

Review: RBM4 was overexpressed and verified by Western blotting.

By Anonymous

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Target Background

Function

RNA-binding factor involved in multiple aspects of cellular processes like alternative splicing of pre-mRNA and translation regulation. Modulates alternative 5'-splice site and exon selection. Acts as a muscle cell differentiation-promoting factor. Activates exon skipping of the PTB pre-mRNA during muscle cell differentiation. Antagonizes the activity of the splicing factor PTBP1 to modulate muscle cell-specific exon selection of alpha tropomyosin. Binds to intronic pyrimidine-rich sequence of the TPM1 and MAPT pre-mRNAs. Required for the translational activation of PER1 mRNA in response to circadian clock. Binds directly to the 3'-UTR of the PER1 mRNA. Exerts a suppressive activity on Cap-dependent translation via binding to CU-rich responsive elements within the 3'UTR of mRNAs, a process increased under stress conditions or during myocytes differentiation. Recruits EIF4A1 to stimulate IRES-dependent translation initiation in respons to cellular stress. Associates to internal ribosome entry segment (IRES) in target mRNA species under stress conditions. Plays a role for miRNA-guided RNA cleavage and translation suppression by promoting association of AGO2-containing miRNPs with their cognate target mRNAs. Associates with miRNAs during muscle cell differentiation. Binds preferentially to 5'-CGCGCG[GCA]-3' motif in vitro.

Gene References into Functions

The impact of the RBM4-initiated splicing cascade on modulating the carcinogenic signature of colorectal cancer cells. PMID: 28276498
hese findings suggest that RBM4 is a new biomarker in gastric cancer, as the reduced expression of this protein is correlated with poor differentiation, lymph node status and distant metastasis PMID: 27324405
RBM4-SRSF3-MAP4K4 constitutes a novel mechanism for manipulating the metastasis of colorectal cancer cells through the JNK1 signaling pathway. PMID: 29138007
TPM1-AS regulates the alternative splicing of TPM1 through an interaction with RBM4 and involves in TPM1-mediated filopodium formation and migration of cancer cells PMID: 28754317
RBM4 is induced and is involved in the PKM splicing switch and neuronal gene expression during hypoxia-induced neuronal differentiation. PMID: 27821480
alternative splicing patterns were altered by knockdown of RBM4 in several types of neoplasms PMID: 26898347
Loss of RBM4 expression is associated with colorectal cancer. PMID: 26506517
The results indicate that the alanine-rich C-terminal domain, in conjunction with its conjoined RNA-binding domain(s), differentially influences the subnuclear localization and biogenesis of RBM4. PMID: 25414336
Up-regulation of RBM4 is associated with breast cancer. PMID: 25140042
RBM4 is a tumor suppressor with therapeutic potential and clinical values as a prognostic factor. PMID: 25203323
we propose the emerging role of RBM4 in regulating the adipocyte-specific splicing events and transcription cascade, which subsequently facilitate the development and function of brown adipocyte-like cells. PMID: 24389249
role in negative feed-forward loop that disrupts inflammattory cytokine translation following Toll-like receptor 4 response PMID: 23897118
RBM4 homologs exert different effects on 5' splice site utilization and exon selection, and exhibit different subnuclear localization patterns. PMID: 23527094
Data show that RBM4 interacts directly with Ago2 during muscle cell differentiation and may recruit Ago2 to suppress translation of target mRNAs. PMID: 19801630
Data show that the nuclear matrix protein matrin 3, cytoskeletal motor protein HMP, and the circadian clock protein lark were significantly decreased in fetal Down syndrome brain. PMID: 12469345
the regulated alternative splicing of alpha-TM by the antagonistic splicing regulators RBM4 and PTB PMID: 16260624
RBM4 is expressed in the human brain regions affected in tauopathy, including the hippocampus and frontal cortex. RBM4 is involved in tau exon 10 alternative splicing PMID: 16777844
Wilms tumor 1 protein, WT1 interacts with the novel splicing regulator RBM4, the longer isoform of WT1 is able to inhibit the effect of RBM4 on alternative splicing. PMID: 16934801
a previously unrecognized paradigm for the RNA-binding protein RBM4 in its phosphorylation-modulated dual action as a suppressor of cap-dependent and enhancer of IRES-mediated translation in response to stress signals PMID: 17284590
human brain, expression of RBM4a was strongly up-regulated in cerebellum as compared to forebrain. PMID: 18708123
RBM4 is an RNA-binding protein involved in diverse cellular processes that include alternative splicing of pre-mRNA, translation, and RNA silencing. PMID: 18723113
The alternative splicing imbalance of CoAA and RBM4, because of loss of their common enhancer in cancer, may deregulate stem/progenitor cell differentiation PMID: 19416963

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Subcellular Location

Nucleus. Nucleus, nucleolus. Nucleus speckle. Cytoplasm. Cytoplasmic granule. Note=Undergoes continuous nucleocytoplasmic shuttling. Upon nuclear import colocalizes with SR proteins in nuclear speckles. Arsenite stress-induced phosphorylation increases its subcellular relocalization from the nucleus to the cytoplasm and to cytoplasmic stress granules (SG) via a p38 MAPK signaling pathway. Primarily localized in nucleus and nucleoli under cell growth conditions and accumulated in the cytoplasm and cytoplasm perinuclear granules upon muscle cell differentiation.

Tissue Specificity

Expressed in the cerebellum. Expressed in neurons and glial cells, including layers II neurons in the frontal cortex and CA1 pyramidal neurons in the hippocampus. Expressed in heart, liver, pancreas, skeletal muscle, placenta, primary fibroblasts and peri

Database Links

HGNC: 9901

OMIM: 602571

KEGG: hsa:5936

STRING: 9606.ENSP00000425446

UniGene: Hs.523822

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