RBPJ Antibody

Code CSB-PA019486GA01HU
Size US$685
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Product Details

Uniprot No. Q06330
Target Names RBPJ
Alternative Names AI843960 antibody; AOS3 antibody; CBF 1 antibody; CBF-1 antibody; CBF1 antibody; csl antibody; IGKJRB antibody; IGKJRB1 antibody; J kappa recombination signal binding protein antibody; J kappa-recombination signal-binding protein antibody; KBF2 antibody; NY REN 30 antigen antibody; RBP J antibody; RBP J kappa antibody; RBP JK antibody; RBP-J antibody; RBP-J kappa antibody; RBP-JK antibody; Rbpj antibody; RBPJK antibody; RBPSUH antibody; recombination signal binding protein for immunoglobulin kappa J region antibody; Recombining binding protein suppressor of hairless antibody; Renal carcinoma antigen NY-REN-30 antibody; SUH antibody; SUH_HUMAN antibody
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Immunogen Human RBPJ
Immunogen Species Homo sapiens (Human)
Isotype IgG
Purification Method Antigen Affinity purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. -20°C, Avoid freeze / thaw cycles.
Tested Applications ELISA,WB,IHC
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA. Binds to the oxygen responsive element of COX4I2 and activates its transcription under hypoxia conditions (4% oxygen)
Gene References into Functions
  1. this report tried to address the molecular basis for the direct interaction between CSL and SMRT. PMID: 30157580
  2. Variants rs2270226 and rs2077777 in the RBPJ gene were associated with the risk of cerebral infarction diseases in the Chinese Han population. PMID: 30075508
  3. RBPJ and MAML3 could be new therapeutic targets for SCLC. PMID: 30061220
  4. The ULK3 Kinase Is Critical for Convergent Control of Cancer-Associated Fibroblast Activation by CSL and GLI PMID: 28877478
  5. Notch1 signaling plays an important role in the maintenance of the cancer stem-like phenotype in diffuse type gastric cancer through an RBP-Jkappa dependent pathway; inhibiting Notch1 signaling could be an effective therapy against CD133 positive diffuse type gastric cancers PMID: 27489358
  6. We show that GIT1, which also contains an ANK domain, inhibits the Notch1-Dll4 signaling pathway by competing with Notch1 ANK domain for binding to RBP-J in stalk cells PMID: 27926858
  7. RBPJ interacts with L3MBTL3 to promote repression of Notch signaling via histone demethylase KDM1A. PMID: 29030483
  8. RBPJ links MYC and transcriptional control through CDK9 in brain tumors, providing potential nodes of fragility for therapeutic intervention, potentially distinct from NOTCH PMID: 27322055
  9. Mean CBF1 expression is significantly increased in isocitrate dehydrogenase 1 (IDH1) R132H mutant glioblastoma. Hypoxic regions of glioblastoma have higher CBF1 activation and exposure to low oxygen can induce its expression in glioma cells in vitro. PMID: 28571041
  10. structural and biophysical studies demonstrate that RITA binds RBP-J similarly to the RAM (RBP-J-associated molecule) domain of Notch, our biochemical and cellular assays suggest that RITA interacts with additional regions in RBP-J. PMID: 28487372
  11. The present findings indicate that p53, in turn, decreases CSL expression, which can serve to enhance p53 activity in acute DNA damage response of cells. PMID: 27163456
  12. RBP-J mediated by miR-133a probably contributed to the regulation of DCs maturation and activation in osteosarcoma PMID: 27794430
  13. These results suggest that PSK suppresses Hedgehog signaling through down-regulation of MAML3 and RBPJ transcription under hypoxia, inhibiting the induction of a malignant phenotype in pancreatic cancer. Our results may lead to development of new treatments for refractory pancreatic cancer using PSK as a Hedgehog inhibitor PMID: 27466498
  14. our findings reconcile the 2 biological events and point to a multistep process of CAF activation under convergent CSL and p53 control. Activation of p53 provides a failsafe mechanism against consequences of compromised CSL activity in stromal cells. PMID: 26735629
  15. RBPJ polymorphism [rs874040(CC) allele] skews memory T cells toward a pro-inflammatory phenotype involving Notch signaling, thus increasing the susceptibility to develop rheumatoid arthritis. PMID: 26604133
  16. The role of CSL-dependent and independent Notch signaling pathways in cell apoptosis is described in normal tissue homeostasis and in tumorigenesis. (Review) PMID: 26496776
  17. These results support a model in which EBNA2 and EBNA3s compete for distinct subsets of RBPJ sites to regulate cell genes and where EBNA3 subset specificity is determined by interactions with other cell transcription factors. PMID: 26719268
  18. Suggest that hypoxia promotes SMO transcription through upregulation of MAML3 and RBPJ to induce proliferation, invasiveness and tumorigenesis in pancreatic cancer. PMID: 26655998
  19. data suggest that shRNA intervention of RBPJ expression could be a promising therapeutic approach for treating human prostate cancer PMID: 26202358
  20. Cancer-associated fibroblast activation-stromal co-evolution is under convergent CSL-p53 control. PMID: 26302407
  21. Single nucleotide polymorphisms in RBPJ, IL1R1, REV3L, TRAF3IP2, IRF1 and ICOS showed association with rheumatoid arthritis in black South Africans. PMID: 25014791
  22. missense mutations and frameshift deletions identified in leukemia patients PMID: 24549417
  23. Our data thus suggest that shRNA intervention of RBPJ expression could be a promising therapeutic approach for treating human lung cancer. PMID: 25589461
  24. RBP-J-interacting and tubulin-associated (RITA) mediates the nuclear export of RBP-J to tubulin fibers and downregulates Notch-mediated transcription. PMID: 25445601
  25. Data indicate that RBPJ protein depletion induces Notch target genes in a Notch-independent fashion. PMID: 25512468
  26. DNA methylation-dependent binding of RBPJ to a GC repressor element can negatively regulate smooth muscl myosin heavy chain promoter activity and can inhibit marker gene expression in phenotypically modulated cells PMID: 25324571
  27. Data suggest that, in B-lymphocytes infected with Epstein-Barr virus (EBV), EBNA3A (EBV nuclear antigen 3A) binds to CBF1-occupied intergenic enhancers located between CXCL10 and CXCL9 and displaces the transactivator EBNA2. PMID: 24068939
  28. KAP1, RBP-Jkappa, and HIF-1alpha play an essential role in KSHV pathogenesis. PMID: 24696491
  29. CBF1/CSL acts as a global hub which is used by the virus to coordinate the lytic cascade. PMID: 23696732
  30. Data indicate that cAMP activates Notch signaling and increases the expression of recombinant recognition sequence binding protein at the Jkappa site (RBP-J) and transducin-like enhancer of Split (TLE). PMID: 23775085
  31. Authors report that human papillomavirus type 8 E6 subverts NOTCH activation during keratinocyte differentiation by inhibiting RBPJ/MAML1 transcriptional activator complexes at NOTCH target DNA. PMID: 23365452
  32. RBPJ regulates rhabdomyosarcoma growth. PMID: 22792167
  33. Characterization of CSL (CBF-1, Su(H), Lag-1) mutants reveals differences in signaling mediated by Notch1 and Notch2. PMID: 22915591
  34. Unique mutations in RBPJ lead to impaired DNA binding in cases of Adams-Oliver syndrome. PMID: 22883147
  35. results indicate that PS2 modulates the degradation of RBP-Jk through phosphorylation by p38 MAPK. PMID: 22302987
  36. Three consensus RBP-Jkappa-binding sites found in the ORF46 promoter are critical for the binding of RBP-Jkappa protein and conferring the ORF50 responsiveness. PMID: 22366521
  37. Association of CSL with NICD exerts remarkably little effect on the exchange kinetics of the ANK domain, whereas MAML1 binding greatly retards the exchange kinetics of ANK repeats 2-3. PMID: 22325781
  38. Bacterial translocation is evident in the colonic mucosa of transgenic RBP-J(DeltaIEC) mice before the onset of colitis, suggesting attenuated epithelial barrier functions in these mice. PMID: 22279105
  39. RBP-Jkappa binding sites within the RTA promoter regulate KSHV latent infection and cell proliferation. PMID: 22253595
  40. RBP-J binds methylated DNA in the context of a mutated RBP-J consensus motif. PMID: 21991380
  41. DNA binding and tetramerization mutants of Rta fail to stimulate RBP-Jk binding to Kaposi's sarcoma-associated herpesvirus DNA. PMID: 21880753
  42. there is a conserved network of cis-regulatory factors that interacts with Notch1 to regulate gene expression in TLL cells, as well as unique classes of divergent RBPJ-only sites that also likely regulate transcription PMID: 21737748
  43. Collectively, our results demonstrate that APP intracellular domain functions as a negative regulator in Notch1 signaling through the promotion of Notch1 and RBP-Jk protein degradation PMID: 21558417
  44. The RBP-Jkappa binding site within the KSHV LANA promoter is crucial for HHV8 latency during early primary infection. PMID: 21507979
  45. These results indicate that EBNA3 proteins interact with multiple RBP/CSL domains, but only N-terminal domain interactions are required for lymphoblastoid cell line growth. PMID: 21440926
  46. data strongly support a model in which EBNA2 association with NCoR-deficient RBPJ enhances transcription and EBNALP dismisses NCoR and RBPJ repressive complexes from enhancers PMID: 21518914
  47. Notch target gene activator RBP-J kappa transgene plays no role in Notch target gene repression in Ikaros double-positive thymocytes during leukemia development. PMID: 20511547
  48. mechanism by which the PhiW PhiP motif of RAM and EBNA2 compete with one another for binding at the hydrophobic pocket of the beta-trefoil domain (BTD) of CSL using overlapping but specific interactions that are unique to each BTD ligand. PMID: 20028974
  49. Studies indicate that the mechanisms that lead to Notch activity in the receiving cell include the cleavage of Notch at the cell membrane and the assembly of a nuclear complex with the transcription factors CSL. PMID: 19247952
  50. The interactions of RBPJ with ORF47 and ORF50 human herpesvirus 8 proteins in transactivation are reported. PMID: 20006367
  51. Inhibition of methylation in EBV-infected cells results in reduced expression of the EBNA2-regulated viral gene LMP1, evidencing that methylation is a prerequisite for DNA-binding by EBNA2 via association with the transcription factor RBPJkappa. PMID: 19969318
  52. Data support a model in which Notch-1 can activate the transcription of ERalpha-target genes via IKKalpha-dependent cooperative chromatin recruitment of Notch-CSL-MAML1 and ERalpha, which promotes the recruitment of p300. PMID: 19838210
  53. SHARP is a novel component of the HDAC corepressor complex, recruited by RBP-Jkappa to repress transcription of target genes in the absence of activated Notch. PMID: 12374742
  54. RBP-J kappa-mediated repression is therefore not essential for establishment of latent KHSV infection, but the RTA-mediated redirection of RBP-J kappa activity from repression to activation is critical for lytic viral replication. PMID: 12832621
  55. Ku antigen interacts with RBP-Jkappa and NF-kappaB p50 may act as a positive regulator of p50 expression in gastric cancer AGS cells. PMID: 14570916
  56. C promoter-binding factor 1 binding is required for Notch-1-mediated repression of activator protein-1 PMID: 14645224
  57. RBP-Jkappa activated a full transcriptional response but only demonstrated partial antiapoptotic activity PMID: 14701863
  58. The ankyrin repeats were also the only domain required for up-regulation of RBP-Jkappa-dependent gene expression PMID: 15187023
  59. regulates Kaposi's sarcoma-associated herpesvirus (KSHV) K14/vGPCR transcripts; this suggests the possibility that other modulators of Notch signaling might be able to induce expression of this RNA outside the context of lytic KSHV replication. PMID: 15194757
  60. Notch promotes changes in hVSMC phenotype via activation of CBF-1/RBP-Jkappa-dependent pathways in vitro and contributes to the phenotypic response of VSMCs to cyclic strain-induced changes in VSMC differentiation. PMID: 15987768
  61. a corepressor complex containing CtIP/CtBP facilitates RBP-Jkappa/SHARP-mediated repression of Notch target genes PMID: 16287852
  62. P48 interacts with the RBP-L and RBP-J subunits primarily through two short conserved tryptophan-containing motifs, similar to the motif of the Notch intracellular domain (NotchIC) that interacts with RBP-J. PMID: 16354684
  63. c-Myc can cooperate with E6/E7 in epithelial transformation and can substitute for CBF1-dependent signals generated by Notch1. PMID: 16378632
  64. Notch/Rbp-j signaling prevents premature differentiation of pancreatic progenitor cells into endocrine and ductal cells during early development of the transgenic pancreas. PMID: 16399505
  65. findings show that induction of FcRH5 by Epstein-Barr virus nuclear antigen 2 is strictly CBF1 dependent PMID: 16439682
  66. Results report the crystal structure of a Notch transcriptional activation complex containing the ankyrin domain of human Notch1, the transcription factor CSL on cognate DNA, and a polypeptide from the coactivator Mastermind-like-1 (MAML-1). PMID: 16530044
  67. EBNA2 trans-activates bfl-1, which requires CBF1 (or RBP-J kappa). PMID: 16873269
  68. RTA activates the Kaposi's sarcoma-associated herpesvirus K8 promoter through an indirect binding mechanism, i.e. being recruited to the K8 promoter through interaction with RBP-Jkappa bound to an RBP-Jkappa motif in the promoter PMID: 17055026
  69. These results support an emerging molecular mechanism for the displacement of co-repressors from DNA-bound CSL by intracellular domain of the Notch receptor . PMID: 17070841
  70. The phosphatase and tensin homolog (PTEN) gene is a direct target of Notch-1 signal transduction, through binding of the Notch-activated transcription factor CBF-1 to the PTEN minimal promoter. PMID: 17245125
  71. A number of Notch-regulated targets are characterized by paired CSL-binding sites in a head-to-head arrangement; cooperative formation of dimeric Notch transcription complexes on promoters with paired sites is required to activate transcription PMID: 17284587
  72. Transcription factor Ying Yang 1 (YY1) indirectly regulates the C promoter-binding factor 1 (CBF1)-dependent Notch1 signaling via direct interaction with the Notch1 receptor. PMID: 17434929
  73. an important relationship exists between zinc and the Notch1 signaling pathway, and that this relationship is intimately involved with the cytoplasmic retention of Notch and RBP-Jk PMID: 17513037
  74. The incorporation of RBP-J kappa into CCAAT-enhancer-binding protein (CEBP) zeta complexes in human hepatoma Hep3B cells is solely to support the binding of CEBP zeta to the CEBP site. PMID: 17513780
  75. the RBP-Jkappa-associated domain of Notch increases the effective concentration of the ankyrin domain for its binding site on CSL, enabling docking of the ankyrin domain and subsequent recruitment of the Mastermind-like coactivator. PMID: 18155729
  76. Present a novel mechanism by which a balance between Notch-1/-2/-4 signaling, via CBF-1, and HRT-1/-2 activity determines the expression of smooth muscle differentiation markers including actin. PMID: 18239137
  77. ETO is part of the endogenous RBP-Jkappa-containing corepressor complex PMID: 18332109
  78. EBNA3C regulation of transcription through RBP-Jkappa is critical to maintaining lymphoblastoid cell growth PMID: 19237563
  79. Both CBF1 and C/EBP-beta bind the CR2 promoter in B cells raising the possibility that these factors facilitate or respond to alterations in chromatin structure to control the timing and/or level of CR2 transcription. PMID: 19487031
  80. The authors confirmed that EBNA3C upregulates TCL1 and discovered that EBNA3C upregulates TCL1 through RBP-Jkappa, indicating a central role for EBNA3C interaction with RBP-Jkappa in mediating cell gene transcription. PMID: 19776126

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Involvement in disease Adams-Oliver syndrome 3 (AOS3)
Subcellular Location Nucleus, Cytoplasm
Protein Families Su(H) family
Database Links

HGNC: 5724

OMIM: 147183

KEGG: hsa:3516

STRING: 9606.ENSP00000345206

UniGene: Hs.479396

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