RNF219 Antibody

Datasheet

Code	CSB-PA019878LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate All lanes: RNF219 antibody at 4μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 81 kDa Observed band size: 81 kDa IHC image of CSB-PA019878LA01HU diluted at 1:300 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA019878LA01HU diluted at 1:300 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) RNF219 Polyclonal antibody

Uniprot No.

Q5W0B1

Target Names

RNF219

Alternative Names

2610206B13Rik antibody; 2810449K13Rik antibody; AI451544 antibody; DKFZp686A01276 antibody; DKFZp686N15250 antibody; DKFZp686O03173 antibody; FLJ13449 antibody; FLJ25774 antibody; MGC35679 antibody; RGD1564706 antibody; RING finger protein 219 antibody; RING finger protein C13orf7 antibody; RN219_HUMAN antibody; RNF219 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human RING finger protein 219 protein (257-465AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The RNF219 Antibody (Product code: CSB-PA019878LA01HU) is Non-conjugated. For RNF219 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA019878LB01HU	RNF219 Antibody, HRP conjugated	ELISA
FITC	CSB-PA019878LC01HU	RNF219 Antibody, FITC conjugated
Biotin	CSB-PA019878LD01HU	RNF219 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:100-1:300

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

E3 ubiquitin ligase essential for DNA replication origin activation during S phase. Acts as a replication origin selector which selects the origins to be fired and catalyzes the multi-mono-ubiquitination of a subset of chromatin-bound ORC3 and ORC5 during S-phase.

Subcellular Location

Chromosome.

Database Links

HGNC: 20308

KEGG: hsa:79596

STRING: 9606.ENSP00000282003

UniGene: Hs.567576

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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