RNF43 Antibody

Code CSB-PA019892LA01HU
Size US$299
  • IHC image of CSB-PA019892LA01HU diluted at 1:600 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

The Latest Promotion Free Antibody trial simple
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) RNF43 Polyclonal antibody
Uniprot No. Q68DV7
Target Names RNF43
Alternative Names
RNF43 antibody; E3 ubiquitin-protein ligase RNF43 antibody; EC antibody; RING finger protein 43 antibody; RING-type E3 ubiquitin transferase RNF43 antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human E3 ubiquitin-protein ligase RNF43 protein (566-697AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated

The RNF43 Antibody (Product code: CSB-PA019892LA01HU) is Non-conjugated. For RNF43 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA019892LB01HU RNF43 Antibody, HRP conjugated ELISA
FITC CSB-PA019892LC01HU RNF43 Antibody, FITC conjugated
Biotin CSB-PA019892LD01HU RNF43 Antibody, Biotin conjugated ELISA
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, IHC
Recommended Dilution
Application Recommended Dilution
IHC 1:500-1:1000
Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis and subsequent degradation of Wnt receptor complex components Frizzled. Acts on both canonical and non-canonical Wnt signaling pathway. Along with RSPO2 and ZNRF3, constitutes a master switch that governs limb specification.
Gene References into Functions
  1. results establish that RSPO2, without the LGR4/5/6 receptors, serves as a direct antagonistic ligand to RNF43 and ZNRF3, which together constitute a master switch that governs limb specification; these findings have direct implications for regenerative medicine and WNT-associated cancers PMID: 29769720
  2. Data suggest that inactivation of RNF43 and ZNRF3 is important in serrated tumorigenesis and has identified a potential therapeutic strategy for this cancer subtype. PMID: 27661107
  3. Chimeric transcript resulting from the fusion of RNF43 and SUPT4H1 was found to occur frequently in primary colorectal carcinoma. PMID: 27461012
  4. RNF43 mutation constitutes an important mutated driver gene for the Serrated Neoplasia pathway in both the sporadic and familial Polyposis settings. PMID: 27329244
  5. RNF43 is down-regulated in intrahepatic cholangiocarcinoma and may play a crucial role during development of ICC. PMID: 26980022
  6. The finding of RNF43 mutation in sessile serrated adenoma and traditional serrated adenoma, but not in tubulovillous/villous adenoma, indicated that RNF43 mutation is an early and specific molecular aberration in the serrated colorectal carcinoma pathway. PMID: 27305845
  7. Mutations and downregulation of RNF43 may play a critical role in the transition from adenoma to carcinoma. PMID: 27514024
  8. PTPRK-RSPO3 fusions and RNF43 mutations were found to be characteristic genetic features of traditional serrated adenomas (TSAs). PMID: 26924569
  9. our study exhibited the expression patterns and correlation of RNF43 and p53 pathway proteins involved in cell cycling PMID: 26823834
  10. Authors also identified ring finger 43 (RNF43), an inhibitor of Wnt/beta-catenin signaling, as a target of miR-550a-5p. PMID: 25961913
  11. RNF43 inhibits the Wnt pathway downstream of oncogenic mutations that activate the pathway. PMID: 26350900
  12. RNF43 might act as a tumor suppressor in gastric carcinoma and might be a potential indicator for the clinical assessment of gastric cancer prognosis PMID: 26184844
  13. RNF43 gene harbored not only exceedingly high mutations but also mutational intratumoral heterogeneity, which together might play a role in tumorigenesis of gastric and colorectal cancers. PMID: 26297255
  14. The nucleoprotein of influenza A virus targets RNF43 to modulate p53 ubiquitination levels and hence causes p53 stabilization which is conducive to an enhanced apoptosis level in the host cells. PMID: 25996295
  15. we found that the decreased expression of RNF43 in glioma was associated with poor prognosis by the Kaplan-Meier survival analysis. Importantly, multivariate analysis suggested RN43 as an independent predictor of overall survival. PMID: 25755738
  16. These results indicate that RNF43 mutation might cause downregulation of the expression of ring finger protein 43 and play a crucial role during development of intraductal papillary mucinous neoplasm of the pancreas. PMID: 25081753
  17. Data indicate Dishevelled (DVL) as a dual function adaptor to recruit negative regulators ZNRF3/RNF43 to Wnt receptors to ensure proper control of pathway activity. PMID: 25891077
  18. Missense mutations of RNF43 change their localization from the endosome to the endoplasmic reticulum. PMID: 25825523
  19. RNF43 is one of the most commonly mutated genes in colorectal and endometrial cancers PMID: 25344691
  20. Signalling potency of R-spondin proteins depends on their ability to recruit ZNRF3 or RNF43 via Fu1 into a complex with LGR receptors, which interact with Rspo via Fu2 PMID: 24225776
  21. RNF43 mutations are recurrent in Chinese patients with mucinous ovarian carcinoma but absent in other subtypes of ovarian cancer. PMID: 24001777
  22. mutational inactivation of RNF43 in pancreatic adenocarcinoma confers Wnt dependency PMID: 23847203
  23. RNF43 is involved in tumorigenesis and progression of human hepatocellular carcinoma. PMID: 23136185
  24. RNF43 is a tumour suppressor gene mutated in mucinous tumours of the ovary. PMID: 23096461
  25. RNF43 and ZNRF3 reduce Wnt signals by selectively ubiquitinating frizzled receptors, thereby targeting these Wnt receptors for degradation PMID: 22895187
  26. These findings suggest that RNF43 is associated with p53-mediated apoptosis in collaboration with NEDL1 in colorectal carcinogenesis. PMID: 21108931
  27. RNF43 may exert its growth promoting effect in an antocrine manner and may be a novel diagnostic marker for colorectal cancer PMID: 15492824
  28. These results infer that RNF43 is a resident protein of the ER and, at least partially, the nuclear membrane, with ubiquitin ligase activity and may be involved in cell growth control potentially through the interaction with HAP95. PMID: 18313049

Show More

Hide All

Involvement in disease Sessile serrated polyposis cancer syndrome (SSPCS)
Subcellular Location Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Nucleus envelope.
Protein Families ZNRF3 family
Tissue Specificity Expressed in fetal kidney, fetal lung, in colon cancer tissues, hepatocellular carcinomas and lung adenocarcinomas. Overexpressed in colorectal cancer cell lines.
Database Links

HGNC: 18505

OMIM: 612482

KEGG: hsa:54894

STRING: 9606.ENSP00000385328

UniGene: Hs.584916

Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
7505 Fannin St Ste 610-322 Houston, TX 77054, USA
Join Us with

Subscribe newsletter

Leave a message

© 2007-2023 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1